Microbes are hardly seen as planktonic species and most commonly found biofilm communities in cases of chronic infections. Biofilms regarded a biological condition, where large group microorganisms gets adhered to biotic or abiotic surface. In this context, Pseudomonas aeruginosa, Gram-negative nosocomial pathogen is the main causative organism responsible for life-threatening persistent infections individuals affected with cystic fibrosis other lung ailments. The bacteria can form strong...

Apigetrin (7-(β-D-glucopyranosyloxy)-4′,5-dihydroxyflavone), a glycoside bioactive dietary flavonoid derived from Taraxacum officinale and Teucrium gnaphalodes, is known to possess anticancer, antioxidant, anti-inflammatory effects on numerous cancers. In the present study, we examined effect of apigetrin in Hep3B hepatocellular cancer cell line (HCC). inhibited growth proliferation cells, as confirmed by MTT colony formation assay. We used at concentrations 0, 50, 100 µM for later...

Scutellarein (SCU), a flavone found in the perennial herb Scutellaria baicalensis, is known for wide range of biological activities. In present study, we investigated effects treatment with SCU flavonoids on inducing apoptosis via extrinsic pathway Hep3B cells. significantly inhibited cell proliferation and induced G2/M phase cycle arrest by inhibiting expression level proteins Cdc25C, cdk1 Cyclin B1. Allophycocyanin (APC)/Annexin V propidium iodide (PI) double-staining showed upregulation...

Sinensetin (SIN) has been reported to exhibit anti-inflammatory and anti-cancer activity. However, the cellular molecular mechanism by which SIN promotes hepatocellular carcinoma (HCC) cell death remains unclear. In present study, we investigated induction of its underlying in HepG2 cells, an HCC line. We found that significantly induced whereas proliferation rate Thle2, human liver epithelial was unaffected SIN. SIN-treated cells were not affected apoptotic death; instead, autophagic...

Scutellarein (SCU) is a well-known flavone with broad range of biological activities against several cancers. Human hepatocellular carcinoma (HCC) major cancer type due to its poor prognosis even after treatment chemotherapeutic drugs, which causes variety side effects in patients. Therefore, efforts have been made develop effective biomarkers the HCC order improve therapeutic outcomes using natural based agents. The current study used SCU as approach HepG2 cell line. Based on viability...

Gastric cancer is the common type of malignancy positioned at second in mortality rate causing burden worldwide with increasing treatment options. Prunetin (PRU) an O-methylated flavonoid that belongs to group isoflavone executing beneficial activities. In present study, we investigated anti-proliferative and cell death effect compound PRU AGS gastric line. The vitro cytotoxic potential was evaluated significant proliferation observed. We identified mechanism due necroptosis through double...

Gastric cancer (GC) is an aggressive malignancy with increased mortality rate and low treatment options. Increasing evidence suggests that network pharmacology will be a novel method for identifying the systemic mechanism of therapeutic compounds in diseases like cancer. The current study aimed to use approach establish predictive targets prunetin-5-O-glucoside (PG) against gastric elucidate its biological mechanisms. Primarily, genes associated pathogenesis GC was identified from DiGeNET...

Iridin is a natural flavonoid found in Belamcanda chinensis documented for its broad spectrum of biological activities like antioxidant, antitumor, and antiproliferative effects. In the present study, we have investigated antitumor potential iridin AGS gastric cancer cells. treatment decreases cell growth promotes G2/M phase cycle arrest by attenuating expression Cdc25C, CDK1, Cyclin B1 proteins. Iridin-treatment also triggered apoptotic death cells, which was verified cleaved Caspase-3 (Cl-...

Inflammation is a multifaceted response of the immune system at site injury or infection caused by pathogens stress via cells. Due to adverse effects chemical drugs, plant-based compounds are gaining interest in current research. Prunetinoside prunetin-5-O-glucoside (PUG) active compound, which possesses anti-inflammatory on In this study, we investigate effect PUG mouse macrophage RAW264.7 cells with without stimulation lipopolysaccharide (LPS). Cytotoxicity results showed that...

In the present study, a polyphenolic mixture was isolated from Seomae mugwort (SM; native Korean variety of Artemisia argyi H.) via extraction with aqueous 70% methanol followed by elution ethyl acetate over silica gel column. Each compound analyzed using high‑performance liquid chromatography coupled tandem mass spectrometry, and compared literature. addition to 14 characterized components, one hydroxycinnamate, six flavonoids, lignan were reported for first time, best our knowledge, in H....

Pectolinarigenin (PEC), a natural flavonoid that is present in citrus fruits, has been reported to exhibit antitumor effects several cancers. Though the mechanism of PEC-induced cytotoxicity documented, proteomic changes are associated with cellular response this poorly understood gastric cancer cells. In study, comparative analysis was performed identify proteins cell death two human lines: AGS and MKN-28. Two-dimensional gel electrophoresis (2-DE) revealed total 29 56 protein spots...

Sarcopenia, the decline of skeletal muscle tissue attributed to primary aging is a major concern in older adults. Flavonoids might have potential benefits by modulating regulation satellite cells, thus preventing loss. Sinensetin (SIN), citrus methylated flavone with anti-inflammatory and anti-proliferative activity, can enhance lipolysis. The objective present study was investigate whether SIN sarcopenia-suppressing effect on cells from thigh calf tissues young old rats.Primary were...

Inflammatory diseases are an important health concern and have a growing incidence worldwide. Thus, developing novel safe drugs to treat these disorders remains pursuit. Artemisia iwayomogi, locally known as Dowijigi (DJ), is perennial herb found primarily in Korea used various such hepatitis, inflammation immune disorders. In the present study, anti-inflammatory effects of polyphenolic extract from DJ flower (PDJ) lipopolysaccharide (LPS)-stimulated mouse macrophage RAW264.7 cells were...

The antioxidant and anti-inflammatory potentials of polyphenols contained in Gynura procumbens (GP) extract were systematically analyzed. Polyphenols GP analyzed for nine peaks using high-performance liquid chromatography (HPLC) combined with mass spectrometry (MS), quantitatively determined through each standard. A total polyphenolic compounds identified the samples their MS data tabulated. To determine potential bioactive ingredients targeting DPPH COX-2, we them by ultrafiltration LC....

Aging is associated with loss of muscle mass and strength that leads to a condition termed sarcopenia. Impaired conditions, morbidity, malnutrition are the factors devaluation fibers in aged animals. Satellite cells play an important role maintaining homeostasis during tissue regeneration repair. Proteomic profiling on skeletal tissues different age group rats helps determine differentially expressed (DE) proteins, which may eventually lead development biomarkers treating conditions In this...

The use of adipose-derived stem cells (ADSCs) to enhance wound healing and tissue regeneration is progressively being accepted. Proteomic profiling cultured ADSCs by mass spectrometry (MS) a valuable tool determine the identity proteins involved in multiple pathways, which make these unique. In current study, Nano-LC-MS/MS analysis was implemented on membrane-free cell component (MFSCC), MS revealed presence 252 proteins, that are several biological functions, like metabolic process,...

Scutellarein (SCU), a flavone that belongs to the flavonoid family and abundantly present in Scutellaria baicalensis flowering plant Lamiaceae, has been reported exhibit anticancer effects several cancer cell lines including gastric (GC). Although our previous study documented mechanisms of Scutellarein‑induced cytotoxic effects, literature shows proteomic changes are associated with cellular response SCU have poorly understood. To avoid adverse side‑effects significant toxicity chemotherapy...