A5079695943- Neonatal Respiratory Health Research
- Parasites and Host Interactions
- Digestive system and related health
- Infant Nutrition and Health
- Immunodeficiency and Autoimmune Disorders
- Congenital gastrointestinal and neural anomalies
- Helicobacter pylori-related gastroenterology studies
- Hormonal Regulation and Hypertension
- Drug-Induced Adverse Reactions
- Retinal Development and Disorders
- Food Allergy and Anaphylaxis Research
- Parasite Biology and Host Interactions
- Pediatric Pain Management Techniques
- Cystic Fibrosis Research Advances
- Allergic Rhinitis and Sensitization
- Microscopic Colitis
- Tissue Engineering and Regenerative Medicine
- Virus-based gene therapy research
- Maternal Mental Health During Pregnancy and Postpartum
- RNA modifications and cancer
- Sepsis Diagnosis and Treatment
- Renal and related cancers
- Wnt/β-catenin signaling in development and cancer
- Contact Dermatitis and Allergies
- Reproductive System and Pregnancy
Boston Children's Hospital
2015-2025
Boston Children's Museum
2015-2025
Harvard University
2018-2024
Dana-Farber/Boston Children's Cancer and Blood Disorders Center
2023-2024
National Jewish Health
2024
University of Colorado Denver
2024
Brigham and Women's Hospital
2021
Center for Biologics Evaluation and Research
2021
KK Women's and Children's Hospital
2021
Duke-NUS Medical School
2021
ABSTRACT Eosinophils and neutrophils contribute to larval killing during the primary immune response, are effector cells in secondary response Strongyloides stercoralis mice. The objective of this study was determine molecular mechanisms used by eosinophils control infections with S. . Using mice deficient eosinophil granule products major basic protein (MBP) peroxidase (EPO), it determined that kill larvae through an MBP-dependent mechanism if other absent. Infecting PHIL mice, which...
Macrophages are multifunctional cells that active in TH1- and TH2-mediated responses. In this study, we demonstrate human mouse macrophages collaborate with neutrophils complement to kill the parasite Strongyloides stercoralis vitro. Infection of mice worms resulted induction alternatively activated (AAM) within peritoneal cavity. These killed vivo collaborated during vitro killing process. AAM generated larvae more rapidly than naive macrophages, which after a longer time period. contrast,...
Abstract Background Mammalian mucosal barriers secrete antimicrobial peptides (AMPs) as critical, host-derived regulators of the microbiota. However, mechanisms that support microbiota homeostasis in response to inflammatory stimuli, such supraphysiologic oxygen, remain unclear. Results We show oxygen exposure neonatal mice, or direct intestinal organoids suppresses expression AMPs and alters composition. Oral supplementation prototypical AMP lysozyme hyperoxia-exposed mice reduced...
Journal Article An Exploration of Redneck Whiteness in Multicultural Canada Get access Anne O'Connell Email: aoconnel@yorku.ca. Search for other works by this author on: Oxford Academic Google Scholar Social Politics: International Studies Gender, State & Society, Volume 17, Issue 4, Winter 2010, Pages 536–563, https://doi.org/10.1093/sp/jxq019 Published: 09 November 2010
Humans with WNT2B deficiency have severe intestinal disease, including significant inflammatory injury, highlighting a critical role for WNT2B. We sought to understand how contributes homeostasis. investigated the health of Wnt2b knock out (KO) mice. assessed baseline histology and small intestine colon, impact challenge using dextran sodium sulfate (DSS). also evaluated human tissue. Mice had normal but enhanced susceptibility DSS colitis because an increased early injury response. Although...
WNT2B is Wnt ligand which able to support intestinal stem cells (ISC) in culture and the epithelium vivo. We have previously shown that critical for resistance colitis, but not small injury, adult mouse. thought coordinate with WNT3 supporting ISC, we also expression low early postnatal ileum mice. Here, hypothesized may be more intestine during development, challenged Wnt2b KO mice controls experimental necrotizing enterocolitis (NEC) on days 5-8. had similar histology injury scores control...
Background and Aims: WNT2B mutations result in Diarrhea-9 (DIAR9), a congenital diarrhea syndrome with an extreme phenotype unique histological defects. Attempts to model DIAR9 rodents study patient epithelial tissue have not been able fully reproduce the human phenotype, making understanding this condition challenging. Here, we aimed interrogate mechanisms specific cellular compartment contributing using intestinal organoid model. Methods: Human organoids (HIOs) generated from both...
Very early onset inflammatory bowel disease (VEO-IBD) represents a diagnostic and treatment challenge. Here we present case of VEO-IBD secondary to mutation in BIRC4 gene, which encodes X-linked inhibitor apoptosis protein (XIAP), 17-month-old boy with severe failure thrive, intractable diarrhea, hepatosplenomegaly. Endoscopy histology identified only mild duodenitis ileitis, but pancolitis crypt abscesses epithelium apoptosis. Minimal improvement symptoms was achieved total parenteral...
Hbs1 has been established as a central component of the cell’s translational quality control pathways in both yeast and prokaryotic models; however, functional characteristics its human ortholog (Hbs1L) have not well-defined. We recently reported novel phenotype resulting from mutation critical coding region HBS1L gene characterized by facial dysmorphism, severe growth restriction, axial hypotonia, global developmental delay retinal pigmentary deposits. Here we further characterize...
WNT2B is a member of the Wnt family, group signal transduction proteins involved in embryologic development and stem cell renewal maintenance. We recently reported homozygous nonsense variants three individuals with severe, neonatal-onset diarrhea, intestinal failure. Here we present fourth case, from separate neonatal diarrhea associated novel compound heterozygous variants. One two was frameshift variant (c.423del [p.Phe141fs]), while other missense change (c.722 G > A [p.G241D]) that...
The steroid hormone aldosterone, produced by the zona glomerulosa (zG) of adrenal gland, is a master regulator plasma electrolytes and blood pressure. While aldosterone control renin-angiotensin system well understood, other key regulatory factors have remained elusive. Here, we replicated prior association between non-coding variant in
Primary immunodeficiency disorders (PIDs) are genetic diseases that lead to increased susceptibility infection. Hundreds of PIDs have now been described, but a select subset commonly presents in the neonatal period. Neonates, especially premature newborns, relative immune immaturity makes it challenging differentiate from intrinsic immaturity. Nonetheless, early identification and appropriate management critical, clinician should be familiar with range their presentations. The also aware...
In the first postnatal month, developing mouse intestine shifts from an immature to a mature that will sustain organism throughout lifespan. Here, we surveyed in C57Bl/6 mice by RNA-Seq evaluate changes gene expression over time day of birth through 1 month age both duodenum and ileum. We analyzed for families correlated with periods NEC susceptibility or resistance. highlight increased DNA processing genes vacuolar structure genes, tissue development morphogenesis cell migration all...
Summary Protection against Mesocestoides corti , a cestode that invades vital organs, is dependent on the production of IL‐4, as IL‐4 −/− mice were found to have higher parasite burdens when compared with wild‐type mice. The goal this study was investigate role in immunity M. focusing immunological profile and potential mediators pathology . infected showed 100% mortality by 32 days, whereas survived for approximately 1 year. Parasite significantly increased liver, peritoneal, thoracic...
We describe a case of paravertebral abscess caused by Phellinus sp. in boy with chronic granulomatous disease. Sequence-based identification this mold, new agent disease, suggests close relation to umbrinellus.
Necrotizing enterocolitis (NEC) is a devastating disease of the neonatal intestine, causing widespread intestinal necrosis as well systemic illness that frequently results in death. Because clinical onset NEC sudden and difficult to predict, considered an acute event. However, does not occur
Wnt/β-catenin signaling is essential for embryonic eye development in both the anterior and retina. WNT2B, a ligand activator of pathway, assists lens peripheral regions eye. In humans WNT2B mutations are associated with coloboma may also assist retinal progenitor cell differentiation chicken, yet potential role neuronal understudied. This study explored effects on vascular formation using systemic Wnt2b knockout (KO) mice generated by crossing Wnt2bflox/flox (fl/fl) CMV-cre mice. KO eyes...