A5076501338- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- Bacteriophages and microbial interactions
- Animal Virus Infections Studies
- SARS-CoV-2 and COVID-19 Research
- Fecal contamination and water quality
- Viral Infections and Immunology Research
- Virus-based gene therapy research
- COVID-19 diagnosis using AI
- Water Treatment and Disinfection
- Respiratory viral infections research
- Advanced biosensing and bioanalysis techniques
- COVID-19 Clinical Research Studies
- Gaze Tracking and Assistive Technology
- Climate Change and Health Impacts
- Gestational Trophoblastic Disease Studies
- Clinical Nutrition and Gastroenterology
- Immune Response and Inflammation
- Prenatal Screening and Diagnostics
- Microbial Community Ecology and Physiology
- Antibiotic Resistance in Bacteria
- Congenital Diaphragmatic Hernia Studies
- Environmental DNA in Biodiversity Studies
- Pharmaceutical and Antibiotic Environmental Impacts
Toyama Prefectural University
2007-2024
The University of Tokyo
2011-2018
Kyoto University
2014-2018
Tokai University
2008
University of Yamanashi
2007
To evaluate and compare the reductions of human viruses F-specific coliphages in a full-scale wastewater treatment plant based on quantitative PCR (qPCR) plate count assays.A total 24 water samples were collected from four locations at plant, relative abundance F-RNA phage genogroups determined by qPCR. Of 10 types tested, enteric adenoviruses most prevalent both influent effluent samples. different steps, activated sludge process was effective reducing microbial loads. Viruses phages showed...
Polyethylene glycol (PEG) precipitation is one of the conventional methods for virus concentration. This technique has been used to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater. The procedures and seeded surrogate viruses were different among implementers; thus, reported whole process recovery efficiencies considerably varied studies. present study compared five PEG procedures, with operational parameters, RT-qPCR-based efficiency murine hepatitis...
ADVERTISEMENT RETURN TO ISSUEPREVViewpointNEXTPotential Sensitivity of Wastewater Monitoring for SARS-CoV-2: Comparison with Norovirus CasesAkihiko HataAkihiko HataFaculty Engineering, Toyama Prefectural University, Imizu 939-0398, JapanMore by Akihiko Hatahttp://orcid.org/0000-0002-9372-5013 and Ryo Honda*Ryo HondaFaculty Geosciences Civil Kanazawa 920-1192, JapanResearch Center Environmental Quality Management, Graduate School Kyoto Otsu 520-0811, Japan*Phone/fax: +81-76-264-6393; email:...
When oral tolerance was induced in either specific pathogen-free (SPF) or germ-free (GF) mice, ovalbumin (OVA) feeding before immunization successfully SPF mice. On the other hand, OVA-specific immunoglobulin G1 (IgG1) and IgE titres OVA-fed GF mice were comparable to those phosphate-buffered saline-fed thus demonstrating that could not be The frequencies of CD25(+) CD4(+)/CD4(+) cells mesenteric lymph node (MLN) absolute number CD4(+) Peyer's patches MLN naive significantly lower than In an...
Inhibitors that reduce viral nucleic acid extraction efficiency and interfere with cDNA synthesis and/or polymerase activity affect the molecular detection of viruses in aquatic environments. To overcome these significant problems, we developed a methodology for assessing yields DNA amplification efficiencies environmental water samples. This involved adding particles adenovirus type 5 murine norovirus newly primer-sharing controls, which are amplified same primer pairs result amplicon sizes...
ABSTRACT Aichi viruses (AiVs) have been proposed as a causative agent of human gastroenteritis potentially transmitted by fecal-oral routes through contaminated food or water. In the present study, we developed TaqMan minor groove binder (MGB)-based reverse transcription-quantitative PCR (RT-qPCR) system that is able to quantify AiVs and differentiate between genotypes A B. This consists two assays, an AiV universal assay utilizing primer pair probe duplex genotype-specific same probes. The...
Abstract Recently reported detection of SARS-CoV-2 in wastewater around the world has led to emerging concerns on potential risk water bodies receiving treated effluent. This review aims provide an up-to-date state key knowledge impact natural wastewater. In this review, concentrations wastewater, expected removal WWTPs, and possible dilution decay are reviewed based past studies related enveloped viruses. We suggest a quantitative microbial assessment (QMRA) framework estimate through...
ABSTRACT Presence of SARS-coronavirus-2 (SARS-CoV-2) in wastewater sample has been documented several countries. Wastewater-based epidemiology (WBE) is potentially effective for early warning COVID-19 outbreak. The purpose this study was to verify the detection limit WBE COVID-19. In total, 27 influent samples were collected from four treatment plants Ishikawa and Toyama prefectures Japan. During period, numbers confirmed cases these increased almost 0 around 20 per 100,000 peoples....
Human astroviruses are associated with gastroenteritis and known to contaminate water environments. Three different genetic clades of infect humans each clade consists diverse strains. This study aimed determine the occurrence diversity astrovirus strains in samples geographical locations, i.e., influent effluent wastewater (n = 24 each) Arizona, U.S., groundwater 37) river 14) collected Kathmandu Valley, Nepal, using next-generation amplicon sequencing. Astrovirus including rare types...
ABSTRACT Human astroviruses (HAstVs) are a common etiological agent of infantile gastroenteritis. Recent studies revealed that novel astrovirus (AstV) strains the MLB clade (MLB-AstVs) and VA (VA-AstVs), which genetically distinct from classic HAstVs, circulating in human population. In present study, we quantified HAstVs as well carried out genetic analysis wastewater Japan. The concentration influent water samples ranged 10 4 to 5 copies per liter, amount removed by treatment was...
Reverse transcription (RT)-PCR-based virus detection from water samples is occasionally hampered by organic substances that are co-concentrated during concentration procedures. To characterize these substances, containing commercially available humic acid, which known to inhibit RT-PCR, and river were subjected adsorption-elution-based using an electronegative membrane. In this study, the before, during, after analyzed in terms of properties efficiencies. Two out three acid solutions...
ABSTRACT F-specific RNA phages (FRNAPHs) are considered potential viral indicators of water pollution due to their occurrence and stability in environments. However, suitability as is not fully elucidated because the characteristics FRNAPHs variable depending on genotype. In this study, for characterization infectious FRNAPH genotypes, integrated culture reverse transcription-PCR coupled with most probable number approach was applied surface samples. Further, recover low concentrations an...
Quantitative detection of pathogenic viruses in the environmental water is essential for assessment safety. It known that some natural organic substances interfere with virus processes, i.e., nucleic acid extraction and reverse transcription-PCR. Such are carried over into a sample after concentration. In this study, inhibitory coastal samples were characterized view their effects on efficiency property as matters. Among 81 tested, 77 (95%) showed low recoveries (<10%) spiked murine...