A5028299740- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 detection and testing
- Fecal contamination and water quality
- Biosensors and Analytical Detection
- SARS-CoV-2 and COVID-19 Research
- Child Nutrition and Water Access
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- Respiratory viral infections research
- Animal Disease Management and Epidemiology
- Parasitic Infections and Diagnostics
- Bacteriophages and microbial interactions
- Vibrio bacteria research studies
- COVID-19 diagnosis using AI
- Pharmaceutical and Antibiotic Environmental Impacts
- Virus-based gene therapy research
- Salmonella and Campylobacter epidemiology
- Antibiotic Resistance in Bacteria
- Groundwater flow and contamination studies
- Antibiotic Use and Resistance
- COVID-19 Clinical Research Studies
- Water Treatment and Disinfection
- Hepatitis Viruses Studies and Epidemiology
- Legionella and Acanthamoeba research
- Congenital Anomalies and Fetal Surgery
Takeda (Japan)
2016-2025
University of Yamanashi
2016-2025
The University of Tokyo
2002-2010
National Institute of Infectious Diseases
2010
National Institute of Public Health
2008-2009
Azabu University
2006
Tohoku University
2006
Wastewater-based epidemiology is a powerful tool to understand the actual incidence of coronavirus disease 2019 (COVID-19) in community because severe acute respiratory syndrome 2 (SARS-CoV-2), etiological agent COVID-19, can be shed feces infected individuals regardless their symptoms. The present study aimed assess presence SARS-CoV-2 RNA wastewater and river water Yamanashi Prefecture, Japan, using four quantitative two nested PCR assays. Influent secondary-treated (before chlorination)...
There is currently a clear benefit for many countries to utilize wastewater-based epidemiology (WBE) as part of ongoing measures manage the coronavirus disease 2019 (COVID-19) global pandemic. Since most wastewater virus concentration methods were developed and validated nonenveloped viruses, it imperative determine efficiency commonly used enveloped severe acute respiratory syndrome 2 (SARS-CoV-2). Municipal seeded with human (CoV) surrogate, murine hepatitis (MHV), was test seven methods:...
ADVERTISEMENT RETURN TO ISSUEPREVViewpointNEXTWastewater-Based Epidemiology: Global Collaborative to Maximize Contributions in the Fight Against COVID-19Aaron BivinsAaron BivinsDepartment of Civil and Environmental Engineering Earth Sciences, University Notre Dame, 156 Fitzpatrick Hall, Indiana 46556, United StatesMore by Aaron Bivins, Devin NorthDevin NorthDepartment North, Arslan AhmadArslan AhmadDepartment Sustainable Development, Science Engineering, KTH Royal Institute Technology,...
ABSTRACT Pepper mild mottle virus (PMMoV) is a plant that has been recently proposed as potential indicator of human fecal contamination environmental waters; however, information on its geographical occurrence in surface water still limited. We aimed to determine the seasonal and geographic PMMoV drinking sources all over Japan. Between July 2008 February 2011, 184 source samples were collected from 30 treatment plants (DWTPs); viruses 1 2 liters each sample concentrated by using an...
The occurrence of human enteric viruses in surface water the Tamagawa River, Japan, was surveyed for 1 year, from April 2003 to March 2004. Sixty-four samples were collected six sites along river, and 500 ml sample concentrated using cation-coated filter method, which developed our previous study. This method showed recovery yields 56% +/- 32% (n = 37) inoculated with polioviruses. More than one kind tested virus detected 43 (67%) 64 by TaqMan PCR. Noroviruses adenoviruses a high positive...
Abstract This study was conducted to evaluate the applicability of crAssphage, pepper mild mottle virus (PMMoV), and tobacco mosaic (TMV) as indicators reduction human enteric viruses during wastewater treatment. Thirty-nine samples were collected from three steps at a treatment plant (raw sewage, secondary-treated final effluent) monthly for 13-month period. In addition indicator viruses, eight [human adenoviruses, JC BK polyomaviruses, Aichi 1 (AiV-1), enteroviruses, noroviruses genogroups...
Polyethylene glycol (PEG) precipitation is one of the conventional methods for virus concentration. This technique has been used to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater. The procedures and seeded surrogate viruses were different among implementers; thus, reported whole process recovery efficiencies considerably varied studies. present study compared five PEG procedures, with operational parameters, RT-qPCR-based efficiency murine hepatitis...
The applicability of wastewater-based epidemiology (WBE) has been extensively studied throughout the world with remarkable findings. This study reports presence and reduction severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) at two wastewater treatment plants (WWTPs) Nepal, along river water, hospital (HWW), from sewer lines collected between July 2020 February 2021. SARS-CoV-2 RNA was detected in 50%, 54%, 100%, 100% water samples WWTPs, hospitals, lines, respectively, by least...
Variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are continuously emerging, highlighting the importance regular surveillance SARS-CoV-2 and other epidemiologically significant pathogenic viruses in current context. Reverse transcription-quantitative PCR (RT-qPCR) is expensive, time-consuming, labor-intensive, requires a large reagent volume, only tests few targets single run. High-throughput qPCR (HT-qPCR) utilizing Biomark HD system (Fluidigm) can be used as an...
Multiplex digital PCR (dPCR) approaches are commonly employed in wastewater-based epidemiology (WBE) studies. However, optimizing the dPCR workflow is a critical step to ensure its reliability and accuracy before application. In this study, 6-plex Crystal Digital PCR® (cdPCR) was optimized for simultaneous detection of six epidemiologically important pathogens, including three enteric viruses, noroviruses genogroups I II (NoV-GI GII) enteroviruses (EnV), respiratory severe acute syndrome...
ABSTRACT A virus concentration method using a cation-coated filter was developed for large-volume freshwater applications. Poliovirus type 1 (LSc 2ab Sabin strain) inoculated into 40 ml of MilliQ (ultrapure) water adsorbed effectively to negatively charged (Millipore HA, 0.45-μm pore size) coated with aluminum ions, 99% (range, 81 114%) which were recovered by elution 1.0 mM NaOH (pH 10.8) following an acid rinse 0.5 H 2 SO 4 3.0). More than 80% poliovirus recovery yields obtained from...
The seasonal profiles of microorganisms in raw sewage, secondary-treated and final effluent at a wastewater treatment plant Tokyo, Japan, were quantitatively determined each month for one year, from July 2003 to June 2004. Human noroviruses, which by real-time PCR, sewage varied 0.17–260 copies/mL genotype 1 2.4–1900 2, showing much higher values winter, the epidemic season. concentration total coliforms, Escherichia coli, or F-specific phages was almost constant throughout year. noroviruses...