A5089121301- Child Nutrition and Water Access
- SARS-CoV-2 detection and testing
- Viral gastroenteritis research and epidemiology
- Water resources management and optimization
- Biosensors and Analytical Detection
- Fecal contamination and water quality
- Water-Energy-Food Nexus Studies
- Water Treatment and Disinfection
- Viral Infections and Immunology Research
- Animal Disease Management and Epidemiology
- COVID-19 epidemiological studies
- Groundwater and Isotope Geochemistry
- Wastewater Treatment and Reuse
- COVID-19 Pandemic Impacts
- SARS-CoV-2 and COVID-19 Research
- Cytomegalovirus and herpesvirus research
- Respiratory viral infections research
- Water Resources and Sustainability
- COVID-19 and Mental Health
- Water Systems and Optimization
- Groundwater flow and contamination studies
- Animal Virus Infections Studies
- Molecular Biology Techniques and Applications
- Groundwater and Watershed Analysis
- Menstrual Health and Disorders
Takeda (Japan)
2014-2025
University of Yamanashi
2013-2025
United Nations University Institute for Sustainability and Peace
2021-2022
United Nations University Institute for the Advanced Study of Sustainability
2021-2022
The University of Tokyo
2021-2022
Wastewater-based epidemiology (WBE) is an approach that can be used to estimate COVID-19 prevalence in the population by detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA wastewater. As WBE uses pooled samples from study population, it inexpensive and non-invasive mass surveillance method compared individual testing. Thus, offers a good complement low- middle-income countries (LMICs) facing high costs of testing or social stigmatization, has huge potential monitor...
Variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are continuously emerging, highlighting the importance regular surveillance SARS-CoV-2 and other epidemiologically significant pathogenic viruses in current context. Reverse transcription-quantitative PCR (RT-qPCR) is expensive, time-consuming, labor-intensive, requires a large reagent volume, only tests few targets single run. High-throughput qPCR (HT-qPCR) utilizing Biomark HD system (Fluidigm) can be used as an...
The role of wastewater-based epidemiology (WBE), a powerful tool to complement clinical surveillance, has increased as many grassroots-level facilities, such municipalities and cities, are actively involved in wastewater monitoring, the testing coronavirus disease 2019 (COVID-19) is downscaled widely. This study aimed conduct long-term surveillance severe acute respiratory syndrome 2 (SARS-CoV-2) Yamanashi Prefecture, Japan, using one-step reverse transcription-quantitative polymerase chain...
Comprehensive data on bacterial and viral pathogens of diarrhea studies applying culture-independent methods for examining antibiotic resistance in wastewater are lacking. This study aimed to simultaneously quantify genes (ARGs), class 1 integron-integrase (int1), diarrhea, 16S rRNA, other indicators using a high-throughput quantitative PCR (HT-qPCR) system. Thirty-six grab samples from treatment plant Japan three times month between August 2022 July 2023 were centrifuged, followed by...
Multiplex digital PCR (dPCR) approaches are commonly employed in wastewater-based epidemiology (WBE) studies. However, optimizing the dPCR workflow is a critical step to ensure its reliability and accuracy before application. In this study, 6-plex Crystal Digital PCR® (cdPCR) was optimized for simultaneous detection of six epidemiologically important pathogens, including three enteric viruses, noroviruses genogroups I II (NoV-GI GII) enteroviruses (EnV), respiratory severe acute syndrome...
Wastewater-based epidemiology (WBE) is a critical tool for monitoring community health. Although much attention has focused on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), causative agent of disease 2019 (COVID-19), other pathogens also pose significant health risks. This study quantified the presence SARS-CoV-2, influenza A virus (Inf-A), and noroviruses genogroups I (NoV-GI) II (NoV-GII) in wastewater samples collected weekly (n = 170) from July 2023 to February 2024 five...
A composite metric assessing water security's physical dimension at the micro/ community level is lacking but essential for setting priorities program and policy implementations. We prepared an objective index (OI) of security to measure using a model centered on household water-use behavior in developing countries' urban areas. cross-sectional survey (n = 1500) with multi-stage cluster design was conducted from December 2015 February 2016 Kathmandu Valley, which has faced long-term, severe...
Information regarding domestic water consumption is vital, as the Kathmandu Valley will soon be implementing Melamchi Water Supply Project; however, updated information on current situation after 2015 Gorkha Earthquake (GEQ) still lacking. We investigated dynamics of pre- and post-GEQ. The piped supply was short, varied widely across Upatyaka Khanepani Limited (KUKL) branches altitude. reduction in piped, ground, jar increase tanker post-GEQ appeared to due impact GEQ. However, did not...
Streptococcus pyogenes, Group A (GAS), is a human pathogen that causes spectrum of diseases from mild to severe, including GAS pharyngitis, common acute respiratory disease in developed countries. Although wastewater-based epidemiology (WBE) has been extensively used monitor viral pathogens such as severe syndrome coronavirus 2, its applicability S. pyogenes remains unexplored. This study was conducted investigate the feasibility detecting and quantifying wastewater by quantitative...
Abstract Decentralized tap water systems are an important drinking source worldwide. A good quality, high-pressure continuous supply (CWS) is always the target of any urban settlement. However, in some areas reported with deteriorated quality even though treated well before supplying. Such deterioration widely from low availability and economically poor countries where supplied intermittently (IWS). This study focuses identifying IWS causes degradation using nitrate-nitrogen (NO 3 -N) as...
Hand, foot, and mouth disease (HFMD) is a highly contagious that primarily affects children under five years of age. It mainly caused by serotypes Enterovirus A (EVA): EVA71, Coxsackievirus types 6 (CVA6), 10 (CVA10), 16 (CVA16). Despite being prevalent in Japan other countries the Asia-Pacific region, few studies have investigated HFMD pathogens wastewater. The present study aimed to develop sensitive broadly reactive quantitative polymerase chain reaction (qPCR) assay dominant serotype...
Depression is emerging as a highly prevalent psychiatric condition among people living with HIV/AIDS (PLWHA). Perceived family support (PFS) buffers depression chronic disease patients. However, similar relationship PLWHA unexplored.To examine the between PFS and in Kathmandu Valley, Nepal.In this cross-sectional study, was measured by Beck Inventory Ia. Nepali Family Support Difficulties Scale. The status of compared 208 HIV-negative participants. examined only PLWHA.Among each...
To develop effective groundwater pollution control strategies for the Kathmandu Valley, Nepal, seasonal variations in microbial quality and their underlying mechanisms must be understood. However, to date, there are no studies that address these topics. In this study, samples from dug wells were collected during dry wet seasons 2009 2012, Escherichia coli (E. coli) total coliforms analysed. Three monitored each month a year. Microbial concentrations shallow significantly higher season than...
Plasmid pBI143, a potential human-specific microbial source tracking marker, has previously been tested only in the USA. This study evaluated performance of pBI143 marker comparison with BacHum, gyrB, and HF183 TaqMan using quantitative PCR on 65 fecal-source samples. Furthermore, it examined reduction by wastewater treatment processes compared to other markers. The analysis included 48 samples for TaqMan, along 17 additional samples, raw secondary-treated sewage (n = 2 each) deer fecal 13)....
Shallow groundwater is the main water source among many alternatives in Kathmandu Valley, Nepal, which has a rapidly growing population and intermittent piped supply. Although human pathogens are detected groundwater, its health effects unclear. We estimated risk of diarrhoea from shallow use using quantitative microbial assessment. Escherichia coli, Giardia cyst Cryptosporidium oocyst levels were analysed dug tube wells samples. E. coli concentrations converted to those enteropathogenic...
Kathmandu Valley faces water scarcity from decades of the added burden storage and treatment, which imposed cost. We estimated method-specific cost in-house drinking treatment (9 L/day used) based on equipment price life, daily operation time, fuel used, consumables replacement frequency, were Nepalese Rupees (NRs) 23, 57, 392, 586 799 for chemicals, ceramic filter, boiling, Euro-Guard reverse osmosis-ultraviolet (RO-UV) purification, respectively. The monthly average was these estimates...