A5062269405- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Chromosomal and Genetic Variations
- Plant Taxonomy and Phylogenetics
- Plant responses to water stress
- Seed Germination and Physiology
- Bioenergy crop production and management
John Innes Centre
2017-2023
Germline defense against transposons Genomes of germ cells present an existential vulnerability to organisms because cell mutations will propagate future generations. Transposable elements are one source such mutations. In the small flowering plant Arabidopsis , Long et al. found that genome methylation in male germline is directed by interfering RNAs (siRNAs) imperfectly transcribed from (see Perspective Mosher). These siRNAs silence and establish inherited patterns sperm, thus maintaining...
Introduction Although DNA methylation patterns are generally considered to be faithfully inherited in Arabidopsis thaliana (Arabidopsis), there is evidence of reprogramming during both male and female gametogenesis. The gynoecium the floral reproductive organ from which ovules develop generate meiotically derived cells that give rise gametophyte. It not known whether can condition genomic ovule or developing Methods We performed whole genome bisulfite sequencing characterize prevail...
Abstract The plant male germline undergoes DNA methylation reprogramming, which methylates genes de novo and thereby alters gene expression facilitates meiosis. Why reprogramming is limited to the how specific are chosen unknown. Here, we demonstrate that genic in germline, from meiocytes sperm, established by germline-specific siRNAs transcribed transposons with imperfect sequence homology. These synthesized meiocyte nurse cells (tapetum) via activity of tapetum-specific chromatin remodeler...
SUMMARY DNA methylation controls eukaryotic gene expression and is extensively reprogrammed to regulate animal development. However, whether developmental reprogramming during the sporophytic life cycle of flowering plants regulates genes presently unknown. Here we report a distinctive, gene-targeted RNA-directed (RdDM) activity in Arabidopsis thaliana male sexual lineage that meiocytes. Loss lineage-specific RdDM causes mis-splicing MPS1/PRD2 gene, thereby disrupting meiosis. Our results...