A5013708786- Chromosomal and Genetic Variations
- DNA Repair Mechanisms
- Photosynthetic Processes and Mechanisms
- Wheat and Barley Genetics and Pathology
- Fungal and yeast genetics research
- Plant Genetic and Mutation Studies
- Plant Disease Resistance and Genetics
- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- Genetic Mapping and Diversity in Plants and Animals
- Genomics and Phylogenetic Studies
- Plant Reproductive Biology
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- Mitochondrial Function and Pathology
- Plant Virus Research Studies
- Plant nutrient uptake and metabolism
- Microtubule and mitosis dynamics
- Plant Pathogens and Resistance
- Genetic diversity and population structure
- Algal biology and biofuel production
- Reproductive biology and impacts on aquatic species
- Allelopathy and phytotoxic interactions
- Salmonella and Campylobacter epidemiology
- Plant responses to water stress
University of Leicester
2016-2025
University of Birmingham
2004-2015
Warwick Hospital
2006
University of Hertfordshire
2002
Johnson Matthey (Germany)
1993
The duplicated Arabidopsis genes ZYP1a/ZYP1b encode closely related proteins with structural similarity to the synaptonemal complex (SC) transverse filament from other species. Immunolocalization detects ZYP1 foci at late leptotene, which lengthen until pachytene fluorescent signals extending entire length of fully synapsed homologs are observed. Analysis zyp1a and zyp1b T-DNA insertion mutants indicates that functionally redundant. SC is not formed in absence prophase I progression...
MSH4 , a meiosis-specific member of the MutS-homolog family genes, is required for normal levels recombination and fertility in budding yeast, mouse, Caenorhabditis elegans . In this paper, we report identification characterization Arabidopsis homolog ( AtMSH4 ). We demonstrate that expression can only be detected floral tissues, consistent with role reproduction. Immunofluorescence studies indicate its limited to early meiotic prophase I, preceding synapsis homologous chromosomes. A T-DNA...
Meiosis involves reciprocal exchange of genetic information between homologous chromosomes to generate new allelic combinations. In cereals, the distribution crossovers, cytologically visible as chiasmata, is skewed toward distal regions chromosomes. However, many genes are known lie within interstitial/proximal low recombination, creating a limitation for breeders. We investigated factors underlying pattern chiasma formation in barley (Hordeum vulgare) and show that reflects polarization...
In this study we have analysed AtASY3, a coiled-coil domain protein that is required for normal meiosis in Arabidopsis. Analysis of an Atasy3-1 mutant reveals loss the compromises chromosome axis formation and results reduced numbers meiotic crossovers (COs). Although frequency DNA double-strand breaks (DSBs) appears moderately Atasy3-1, main recombination defect reduction COs. Immunolocalization studies wild-type meiocytes indicate HORMA AtASY1, which related to Hop1 budding yeast, forms...
Summary Meiotic crossovers/chiasmata, that are required to ensure chromosome disjunction, arise via the class I interference‐dependent pathway or II interference‐free pathway. The proportions of these two classes vary considerably between different organisms. In Arabidopsis, about 85% chiasmata eliminated in Atmsh4 mutants, denoting events. budding and fission yeasts Msh4‐independent crossovers largely entirely a Mus81‐dependent To investigate origins 15% residual (AtMSH4‐independent)...
MSH5, a meiosis-specific member of the MutS-homologue family genes, is required for normal levels recombination in budding yeast, mouse and Caenorhabditis elegans. In this paper we report identification characterization Arabidopsis homologue MSH5 (AtMSH5). Transcripts AtMSH5 are specific to reproductive tissues, immunofluorescence studies indicate that expression protein abundant during prophase I meiosis. T-DNA tagged insertional mutant (Atmsh5-1), reduced about 13% wild-type levels. The...
Meiotic chromosomes are organized into linear looped chromatin arrays by a protein axis localized along the loop-bases. Programmed remodelling of occurs during prophase I meiosis. Structured illumination microscopy (SIM) has revealed dynamic changes in chromosome Arabidopsis thaliana and Brassica oleracea. We show that associated ASY1 is depleted zygotene concomitant with synaptonemal complex (SC) formation. Study an Atpch2 mutant demonstrates this requires conserved AAA+ ATPase, PCH2, which...
Significance The synaptonemal complex (SC) is a meiosis-specific proteinaceous ultrastructure required to ensure cross-over (CO) formation in the majority of sexually reproducing eukaryotes. It composed two lateral elements adjoined by transverse filaments. Even though general structure SC conserved throughout kingdoms, phenotypic differences between mutants perpetuate enigmatic role SC. Here, we have used genetic and cytogenetic approaches show that filament protein, ZYP1, acts on multiple...
Abstract Adaptive gene flow is a consequential phenomenon across all kingdoms. Although recognition increasing, there no study showing that bidirectional mediates adaptation at loci manage core processes. We previously discovered concerted molecular changes among interacting members of the meiotic machinery controlling crossover number upon to whole-genome duplication (WGD) in Arabidopsis arenosa . Here we conduct population genomic test hypothesis WGD has been mediated by adaptive between...
The human hereditary disease Fanconi anemia leads to severe symptoms, including developmental defects and breakdown of the hematopoietic system. It is caused by single mutations in FANC genes, one which encodes DNA translocase FANCM (for complementation group M), required for repair interstrand cross-links ensure replication progression. We identified a homolog Arabidopsis thaliana that not directly involved lesions but suppresses spontaneous somatic homologous recombination via RecQ...
Sequence exchange between homologous chromosomes through crossing over and gene conversion is highly conserved among eukaryotes, contributing to genome stability genetic diversity. A lack of recombination limits breeding efforts in crops; therefore, increasing rates can reduce linkage drag generate new combinations.We use computational analysis 13 recombinant inbred mapping populations assess crossover frequency the hexaploid wheat (Triticum aestivum). We observe that high-frequency sites...
In many cereal crops, meiotic crossovers predominantly occur toward the ends of chromosomes and 30 to 50% genes rarely recombine. This limits exploitation genetic variation by plant breeding. Previous reports demonstrate that chiasma frequency can be manipulated in plants depletion synaptonemal complex protein ZIPPER1 (ZYP1) but conflict as direction change, with fewer chiasmata reported Arabidopsis thaliana more for rice (Oryza sativa). Here, we use RNA interference (RNAi) reduce amount...
Meiosis recombines genetic variation and influences eukaryote genome evolution. During meiosis, DNA double-strand breaks (DSBs) enter interhomolog repair to yield crossovers noncrossovers. DSB occurs as replicated sister chromatids are connected a polymerized axis. Cohesin rings containing the REC8 kleisin subunit bind anchor chromosomes Here, we report genomic landscape of using chromatin immunoprecipitation sequencing (ChIP-seq) in Arabidopsis (Arabidopsis thaliana). associates with...
Article16 September 2020Open Access Transparent process MSH2 shapes the meiotic crossover landscape in relation to interhomolog polymorphism Arabidopsis Alexander R Blackwell orcid.org/0000-0002-0369-4584 Department of Plant Sciences, University Cambridge, UK Search for more papers by this author Julia Dluzewska orcid.org/0000-0002-7419-5934 Laboratory Genome Biology, Institute Molecular Biology and Biotechnology, Adam Mickiewicz University, Poznan, Poland Maja Szymanska-Lejman...
F-box proteins constitute a large superfamily in plants and play important roles controlling many biological processes, but the of male meiosis remain unclear. Here, we identify rice (Oryza sativa) gene MEIOTIC F-BOX (MOF), which is essential for meiotic progression. MOF belongs to FBX subfamily predominantly active during leptotene pachytene prophase I. mof meiocytes display disrupted telomere bouquet formation, impaired pairing synapsis homologous chromosomes, arrested at late I, followed...
Abstract FANCM suppresses crossovers in plants by unwinding recombination intermediates. In wheat, are skewed toward the chromosome ends, thus limiting generation of novel allelic combinations. Here, we observe that maintains obligate crossover tetraploid and hexaploid ensuring every pair exhibits at least one crossover, localizing class I protein HEI10 pachytene. also II increased 2.6-fold fancm msh5 quadruple mutants. These data consistent with a role for second-end capture designated...