A5084513440- Liver physiology and pathology
- Liver Disease Diagnosis and Treatment
- Pancreatic function and diabetes
- Pluripotent Stem Cells Research
- Tissue Engineering and Regenerative Medicine
- CRISPR and Genetic Engineering
- Organ Transplantation Techniques and Outcomes
- Growth Hormone and Insulin-like Growth Factors
- Cancer, Hypoxia, and Metabolism
- Renal and related cancers
- Virus-based gene therapy research
- Endoplasmic Reticulum Stress and Disease
- Adipose Tissue and Metabolism
- Congenital heart defects research
- RNA and protein synthesis mechanisms
- Reproductive Biology and Fertility
- Cancer Cells and Metastasis
- Liver Disease and Transplantation
- 3D Printing in Biomedical Research
- Muscle Physiology and Disorders
- Drug Transport and Resistance Mechanisms
- Tendon Structure and Treatment
- Hippo pathway signaling and YAP/TAZ
- Diet, Metabolism, and Disease
- Innovation and Socioeconomic Development
University of Pittsburgh
2015-2022
Matrix Research (United States)
2019
Consumer Healthcare Products Association
2015
Institut Cochin
2011-2014
Sorbonne Paris Cité
2014
Université Paris Cité
2011-2014
Centre National de la Recherche Scientifique
2011-2014
Inserm
2011-2014
Délégation Paris 5
2011-2014
Cochin University of Science and Technology
2011
The availability of an autologous transplantable auxiliary liver would dramatically affect the treatment disease. Assembly and function in vivo a bioengineered human derived from induced pluripotent stem cells (iPSCs) has not been previously described. By improving methods for decellularization, recellularization, differentiation different cellular lineages iPSCs organ-like environment, we generated functional engineered mini livers performed transplantation rat model. Whereas previous...
Differentiation of stem cells to hepatocyte-like (HLCs) holds great promise for basic research, drug and toxicological investigations, clinical applications. There are currently no protocols the production HLCs from cells, such as embryonic or induced pluripotent that produce fully mature hepatocytes with a wide range hepatic functions. This report describes standard method assess maturation cell-derived moderately high-throughput format, by analysing liver gene expression quantitative...
GH is a pleiotropic hormone that plays major role in proliferation, differentiation, and metabolism via its specific receptor. It has been previously suggested signaling pathways are required for normal liver regeneration but the molecular mechanisms involved have yet to be determined. The aim of this study was identify by which controls regeneration. We performed two thirds partial hepatectomies receptor (GHR)-deficient mice wild-type littermates showed blunted progression G1/S transition...
GH pathway has been shown to play a major role in liver regeneration through the control of epidermal growth factor receptor (EGFR) activation. This is down-regulated nonalcoholic fatty disease. Because known be impaired livers, we wondered whether deregulation GH/EGFR could explain this deficiency. Hepatic EGFR expression and triglyceride levels were quantified biopsies 32 obese patients with different degrees steatosis. We showed significant inverse correlation between level hepatic...
There are unprecedented epidemics of obesity, such as type II diabetes and non-alcoholic fatty liver diseases (NAFLD) in developed countries. A concerning percentage American children being affected by obesity NAFLD. Studies have suggested that the maternal environment utero might play a role development these later life. In this study, we documented inhibiting SIRT1 signaling human fetal hepatocytes rapidly led to an increase intracellular glucose lipids levels. More importantly, both de...
It has been suggested that increasing age is correlated with an acceleration of the progression liver fibrosis induced by various agents, such as hepatitis C virus or chronic alcohol consumption. However, cellular and molecular changes underlying this predisposition are not entirely understood. In context aging population, it becomes challenging to decipher mechanisms responsible for higher susceptibility older individuals acquired disorder. To address issue, we carbon tetrachloride (CCl(4))...
Organ-like microenviroment and 3-dimensional (3D) cell culture conformations have been suggested as promising approaches to mimic in a micro-scale whole organ cellular functions interactions present vivo. We used this approach examine biologic features of hepatocellular carcinoma (HCC) cells. In study, we demonstrate that cells, fibroblasts, endothelial cells extracellular matrix can generate organoid-like spheroids enhanced numerous human HCC observed show the addition non-parenchymal such...
The mechanisms by which the liver fails in end‐stage disease remain elusive. Disruption of transcription factor network hepatocytes has been suggested to mediate terminal failure animals. However, this hypothesis remains unexplored human subjects. To study relevance expression stages chronic humans, we analyzed liver‐enriched factors (LETFs) hepatocyte nuclear (HNF)4α, HNF1α, forkhead box protein A2 (FOXA2), CCAAT/enhancer‐binding (CEBP)α, and CEBPβ. We then selected downstream genes...
Unraveling the molecular clues of liver proliferation has become conceivable thanks to model two-third hepatectomy. The synchronicity and well-scheduled aspect this process allow scientists slowly decipher mystery. During phenomenon, quiescent hepatocytes remnant lobes are able reenter into cell cycle initiating G1-S progression synchronously before completing cycle. major role played by step been emphasized loss-of-function studies showing a delay or lack coordination in progression. Two...
Hepatocyte nuclear factor 4 alpha (HNF4α) is a transcription that plays critical role in hepatocyte function, and HNF4α‐based reprogramming corrects terminal liver failure rats with chronic disease. In the livers of patients advanced cirrhosis, HNF4α RNA expression levels decrease as hepatic function deteriorates, protein found cytoplasm. These findings could explain impaired degenerative this study, we analyzed localization pathways involved post‐translational modification human hepatocytes...
Auxiliary partial liver transplantation (APLT) in humans is a therapeutic modality used especially to treat failure children or congenital metabolic disease. Animal models of APLT have helped explore options. Though many groups suggested improvements, standardizing the surgical procedure has been challenging. Additionally, question whether graft livers are reconstituted by recipient-derived cells after controversial. The aim this study was improve experimental rats and assess cell...
Abstract Programmable epigenetic modulators provide a powerful toolkit for controlling gene expression in novel therapeutic applications, but recent discovery efforts have primarily selected potency of effect rather than contextual robustness or durability thereof. Current CRISPR-based tools are further limited by large cargo sizes that impede clinical delivery and, activation contexts, brief activity windows preclude transient, single-dose strategies such as lipid nanoparticle (LNP)...