A5005368488- Electron and X-Ray Spectroscopy Techniques
- Ion-surface interactions and analysis
- Advanced Electron Microscopy Techniques and Applications
- Vibrio bacteria research studies
- Metal and Thin Film Mechanics
- Wnt/β-catenin signaling in development and cancer
- Antibiotic Resistance in Bacteria
- Retinal Diseases and Treatments
- Pluripotent Stem Cells Research
- Erythrocyte Function and Pathophysiology
- RNA Research and Splicing
- Renal and related cancers
- Yersinia bacterium, plague, ectoparasites research
- Bacterial biofilms and quorum sensing
- Retinal Development and Disorders
- Bacillus and Francisella bacterial research
- Autophagy in Disease and Therapy
- Advanced Fluorescence Microscopy Techniques
- Genetic and Kidney Cyst Diseases
- Cystic Fibrosis Research Advances
- Occupational exposure and asthma
- Mass Spectrometry Techniques and Applications
- Occupational and environmental lung diseases
- Adipose Tissue and Metabolism
- Viral Infections and Outbreaks Research
University of Pittsburgh
2013-2025
University of Pittsburgh Medical Center
2009-2020
Legacy Health
2012
Tanta University
2012
Biolog (United States)
2011
Harlow College
1961
Birkbeck, University of London
1953
Chronic obstructive pulmonary disease (COPD) involves aberrant airway inflammatory responses to cigarette smoke (CS) that are associated with epithelial cell dysfunction, cilia shortening, and mucociliary clearance disruption. Exposure CS reduced length induced autophagy in vivo differentiated mouse tracheal cells (MTECs). Autophagy-impaired (Becn1+/- or Map1lc3B-/-) mice MTECs resisted CS-induced shortening. Furthermore, increased the autophagic turnover of ciliary proteins, indicating may...
The availability of an autologous transplantable auxiliary liver would dramatically affect the treatment disease. Assembly and function in vivo a bioengineered human derived from induced pluripotent stem cells (iPSCs) has not been previously described. By improving methods for decellularization, recellularization, differentiation different cellular lineages iPSCs organ-like environment, we generated functional engineered mini livers performed transplantation rat model. Whereas previous...
Vascularization plays a critical role in organ maturation and cell type development. Drug discovery, mimicry, ultimately transplantation hinge on achieving robust vascularization of vitro engineered organs. Here, focusing human kidney organoids, we overcame this hurdle by combining induced pluripotent stem (iPSC) line containing an inducible ETS translocation variant 2 (ETV2) (a transcription factor playing endothelial development) that directs differentiation vitro, with non-transgenic iPSC...
Abstract Non-neovascular or dry age-related macular degeneration (AMD) is a multi-factorial disease with of the aging retinal-pigmented epithelium (RPE). Lysosomes play crucial role in RPE health via phagocytosis and autophagy, which are regulated by transcription factor EB/E3 (TFEB/E3). Here, we find that increased AKT2 inhibits PGC-1α to downregulate SIRT5, identify as an binding partner. Crosstalk between SIRT5 facilitates TFEB-dependent lysosomal function RPE. AKT2/SIRT5/TFEB pathway...
The mechanistic target of rapamycin (mTOR) complexes 1 and 2 (mTORC1/2) are crucial for various physiological functions. Although the role mTORC1 in retinal pigmented epithelium (RPE) homeostasis age-related macular degeneration (AMD) pathogenesis is established, function mTORC2 remains unclear. We investigated both RPE health disease. Therefore, this study, we have attempted to demonstrate that specific overexpression mammalian lethal with Sec13 protein 8 (mLST8) mouse activates mTORC2,...
The penetration of electrons energies between 10 and 40 kev into a number phosphors, the extent their scattering, is measured on microphotographs luminous region produced in phosphor by beam less than 0.75 μ diameter. A description given apparatus electron optical system designed to produce fine focal spot. phosphors used experiment were single crystals thallium-activated iodides K, Rb Cs, tungstates Ca Cd, luminescent plastic. An attempt made account for discrepancies ranges as found...
Beta-catenin plays important roles in liver physiology and hepatocarcinogenesis. While studying the role of β-catenin diet-induced steatohepatitis, we recently found that liver-specific knockout (KO) mice exhibit intrahepatic cholestasis. This study was undertaken to further characterize biliary physiology. KO wild-type (WT) littermates were fed standard chow or a diet supplemented with 0.5% cholic acid for 2 weeks. Chow-fed had higher serum hepatic total bile levels lower flow rate than WT...
Occupational exposure to crystalline silica is a well-established occupational hazard. Once in the lung, particles can result activation of alveolar macrophages (AM), potentially leading silicosis, fibrotic lung disease. Because beginning step complicated inflammatory cascade, it necessary define particle characteristics resulting this activation. The aim research was determine effect size on macrophages. RAW 264.7 were exposed four different sizes and their measured using electron...
Viral cancers show oncogene addiction to viral oncoproteins, which are required for survival and proliferation of the dedifferentiated cancer cell. Human Merkel cell carcinomas (MCCs) that harbor a clonally integrated polyomavirus (MCV) genome have low mutation burden require T antigen expression tumor growth. Here, we showed MCV + MCC cells cocultured with keratinocytes undergo neuron-like differentiation neurite outgrowth, secretory vesicle accumulation, generation sodium-dependent action...
Motile cilia project from the airway apical surface and directly interface with inhaled external environment. Owing to cilia's nanoscale dimension high beating frequency, quantitative assessment of their motility remains a sophisticated task. Here we described robust approach for reproducible engineering apical-out organoid (AOAO) defined number cells. Propelled by exterior-facing beating, mature AOAO exhibited stable rotational motion when surrounded Matrigel. We developed computational...
Klebsiella pneumoniae carbapenemase-producing K. (KPC-Kp) bloodstream infections are associated with high mortality. We studied clinical KPC-Kp isolates to investigate mechanisms of resistance complement, a key host defense against infection.
Purpose: To evaluate the endothelial cell survival and stromal bed quality when creating deep cuts with a low–pulse energy, high-frequency femtosecond laser to produce “ultrathin” tissue for Descemet stripping automated keratoplasty. Methods: Seventeen corneas were used this study. Five cut at depth of 420 500 μm thickness approximately ≤70 μm. served as an uncut comparison group. Vital dye staining computer digitized planimetry analysis performed on these corneas. The 7 remaining scanning...
Transfusion of blood at the limits approved storage time is associated with lower red cell (RBC) post-transfusion recovery and hemolysis, which increases plasma cell-free hemoglobin iron, proposed to induce endothelial dysfunction impair host defense. There noted variability among donors in intrinsic rate changes RBC recovery, yet genetic determinants that modulate this process are unclear.We explore stability murine models allogeneic xenogeneic transfusion using from humanized transgenic...
A polyphasic taxonomic study was carried out on strain T5W1T, isolated from the roots of aquatic plant Spirodela polyrhiza. This isolate is Gram-negative, rod-shaped, motile, aerobic and non-pigmented. Nearly complete 16S rRNA gene sequence homology related to Pseudomonas, with 98.4% similarity P. guineae, peli leptonychotis. Average nucleotide identity (ANI) digital DNA-DNA hybridization (dDDH) closest phylogenetic neighbour T5W1T showed differences at species level, further confirmed by in...
A polyphasic taxonomic study was carried out on strain T9W2-OT, isolated from the roots of aquatic plant Salvinia minima. This isolate is rod-shaped, forms yellow/orange pigmented colonies and produces pigment flexirubin. Nearly complete 16S rRNA gene sequence homology related to Chryseobacterium, with 98.8 98.5% similarity Chryseobacterium profundimaris takakiae, respectively. Average nucleotide identity digital DNA-DNA hybridization closest phylogenetic neighbour T9W2-OT showed differences...
Abstract Cell-cell communication underlies all emergent properties of the brain, including cognition, learning and memory. The physical basis for these communications is synapse, a multi-component structure requiring coordinated interactions between diverse cell types. However, many aspects three-dimensional (3D) synaptic organization remain poorly understood. Here, we developed an approach, seamless in situ trimming milling (SISTM), to reliably fabricate sufficiently thin lamellae mapping...
The operation of these dc-energized cold cathode sources is based on the effect that electrons describe long oscillatory paths in presence an electrostatic saddle potential field. A high probability thus exists ionizing any gas present source, to maintain a discharge at considerably lower pressures (10−4–10−3 Torr) than conventional tubes without magnetic Depending symmetry saddle-field, beams various cross-sections are obtained: axial field produces high-intensity pencil beam (2 mm...
Francisella tularensis is the causative agent of tularemia and classified as a category A biodefense by Centers for Disease Control Prevention because its highly infectious nature. F. infects leukocytes exhibits an extracellular phase in blood host. It unknown, however, whether can infect erythrocytes; thus, we examined this possibility vivo vitro. In murine model pulmonary type tularemia, showed presence intraerythrocytic bacteria double-immunofluorescence microscopy ex gentamicin...
We used controlled cortical impact in mice to model human traumatic brain injury (TBI). Local was accompanied by distal diaschisis lesions that developed within regions anatomically connected the injured cortex. At 7 days after injury, histochemistry documented broadly distributed lesions, particularly contralateral cortex and ipsilateral thalamus striatum. Reactive astrocytosis microgliosis were noted multiple neural pathways also showed silver-stained cell processes bodies. Wisteria...