A5073936664- Polyomavirus and related diseases
- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- Cancer-related molecular mechanisms research
- Cancer Cells and Metastasis
- Antenna Design and Analysis
- Cancer, Hypoxia, and Metabolism
- RNA Research and Splicing
- Genomics and Phylogenetic Studies
- Single-cell and spatial transcriptomics
- Histone Deacetylase Inhibitors Research
- Full-Duplex Wireless Communications
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Cutaneous lymphoproliferative disorders research
- CAR-T cell therapy research
- Cancer Research and Treatments
- Energy Harvesting in Wireless Networks
- RNA and protein synthesis mechanisms
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Plant Disease Resistance and Genetics
- Tumors and Oncological Cases
- Nanoplatforms for cancer theranostics
- Glioma Diagnosis and Treatment
Deutschen Konsortium für Translationale Krebsforschung
2019-2024
German Cancer Research Center
2019-2023
Heidelberg University
2019-2023
University Hospital Heidelberg
2023
DKFZ-ZMBH Alliance
2023
German Marine Research Consortium
2023
University of Duisburg-Essen
2021-2022
Essen University Hospital
2019-2022
University of Cologne
2016
Viral cancers show oncogene addiction to viral oncoproteins, which are required for survival and proliferation of the dedifferentiated cancer cell. Human Merkel cell carcinomas (MCCs) that harbor a clonally integrated polyomavirus (MCV) genome have low mutation burden require T antigen expression tumor growth. Here, we showed MCV + MCC cells cocultured with keratinocytes undergo neuron-like differentiation neurite outgrowth, secretory vesicle accumulation, generation sodium-dependent action...
Merkel cell carcinoma (MCC) is a rare, highly aggressive skin cancer for which immune modulation by checkpoint inhibitors shows remarkable response rates. However, primary or secondary resistance to immunotherapy prevents benefits in significant proportion of patients. For MCC, one escape mechanism insufficient recognition T cells owing the downregulation major histocompatibility complex I surface expression. Histone deacetylase have been demonstrated epigenetically reverse low expression...
Abstract Merkel cell carcinoma (MCC) is a highly invasive and metastatic skin cancer. While high expression of miR-375 characteristic MCC, it seems not to contribute the malignant phenotype MCC cells. enrichment in MCC-derived extracellular vesicles suggests its intercellular signaling function. Here, we demonstrate that horizontally transferred causes fibroblast polarization toward cancer-associated fibroblasts (CAFs). The evidenced by phenotypic changes induction α-SMA , CXCL2 IL-1β ....
Abstract Background Intratumoral heterogeneity is crucially involved in metastasis, resistance to therapy, and cancer relapse. Amplifications of the proto-oncogene MYC display notable at single-cell level are associated with a particularly dismal prognosis high-risk medulloblastomas (MBs). The aim this study was establish relevance interclonal cross-talk between MYC-driven non-MYC-driven MB cells. Methods We used fluorescence situ hybridization, transcriptomics, immunohistochemistry, vitro...
Abstract Background Oral squamous cell carcinoma (OSCC), an HPV-negative head and neck cancer, frequently metastasizes to the regional lymph nodes but only occasionally beyond. Initial phases of metastasis are associated with epithelial–mesenchymal transition (EMT), while consolidation phase is mesenchymal–epithelial (MET). This dynamic referred as plasticity (EMP). While it known that EMP essential for cancer invasion metastatic spread, less about heterogeneity states even between primary...
Homologous long non-coding RNAs (lncRNAs) are elusive to identify by sequence similarity due their fast-evolutionary rate. Here we develop LincOFinder, a pipeline that finds conserved intergenic lncRNAs (lincRNAs) between distant related species means of microsynteny analyses. Using this tool, have identified 16 bona fide homologous lincRNAs the amphioxus and human genomes. We characterized compared in Xenopus expression domain one them, Hotairm1, located anterior part Hox cluster. In...
Abstract Merkel cell carcinoma (MCC) is a neuroendocrine tumor either induced by integration of the polyomavirus into genome or accumulation UV-light-associated mutations (VP-MCC and UV-MCC). Whether VP- UV-MCC have same different cellular origins unclear; with mesenchymal epidermal discussed. DNA-methylation patterns proven utility in determining cancers. Therefore, we used this approach to uncover evidence regarding origin classical lines, i.e., lines growth pattern ( n = 9 4,...
Gene duplication is an important mechanism of molecular evolution. It offers a fast track to modification, diversification, redundancy or rescue gene function. However, may also be neutral (slightly) deleterious, and often ends in pseudo-geneisation. Here, we investigate the phylogenetic distribution ultra large families on long short evolutionary time scales. In particular, focus family NACHT-domain leucine-rich-repeat-containing (NLR)-genes, which previously found numbers occupy one...
Despite remarkable advances in treating patients with metastatic melanoma, management of melanoma brain metastases remains challenging. Recent evidence suggests that epigenetic reprogramming is an important mechanism for the adaptation cells to environment. In this study, methylomes and transcriptomes a cohort matched were evaluated by integrated omics data analysis. The identified 38 candidate genes displayed distinct promoter methylation corresponding gene expression changes intracranial...
Class I selective histone deacetylase inhibitors (HDACi) have been previously demonstrated to not only increase major histocompatibility complex class surface expression in Merkel cell carcinoma (MCC) cells by restoring the antigen processing and presentation machinery, but also exert anti-tumoral effect inducing apoptosis. Both phenomena could be due induction of type interferons (IFN), as has described for HDACi. However, mechanism IFN under HDACi is fully understood because IFNs regulated...
The relevance of Hedgehog signaling in Merkel cell carcinoma has only been addressed by a few studies with conflicting results. Thus, we aimed to establish the expression molecules characterize causes aberrant and correlate these findings clinical course patients. Immunohistochemistry was performed for Sonic, Indian, Patched 1 (PTCH1) Smoothened on patients' tumor tissue. Respective mRNA analyzed 10 lines using quantitative real-time polymerase chain reaction. PTCH1 sequencing DNA...
Inflammatory bowel disease such as chronic colitis promotes colorectal cancer, which is a common cause of cancer mortality worldwide. Hypoxia characteristic inflammation well solid tumors and enforces gene expression response controlled by hypoxia-inducible factors (HIFs). Once established, are immunosuppressive to escape their abatement through immune cells. Although HIF activity known 1) promote development 2) drive tumor suppression the secretion adenosine, both prolyl hydroxylases an...
Sézary Syndrome (SS) is an aggressive leukemic variant of cutaneous T-cell lymphomas (CTCL). In SS patients, malignant T cells are circulating through the blood and cause erythroderma. To compare transcriptome single in skin samples from a patient with advanced SS. We utilized combined cell RNA receptor (TCR) sequencing (scRNA-seq). scrutinized unbiased manner without pre-sorting cells. observed different phenotypes same monoclonal population, confirmed by TCR inferred copy number variation...
Merkel cell carcinoma (MCC) is an aggressive skin tumour with neuroendocrine differentiation. Immunotherapies are effective in the treatment of patients advanced-stage MCC, but for whose tumours cannot be controlled by immune system, alternative approaches urgently needed.To identify overexpressed oncogenes as potential drug targets MCC.NanoString platform, digital droplet polymerase chain reaction (ddPCR) and fluorescence situ hybridization (FISH) assays were used to determine copy number...
Abstract Background Oral squamous cell carcinoma (OSCC), an HPV-negative head and neck cancer, frequently metastasizes to the regional lymph nodes but only occasionally beyond. Initial phases of metastasis are associated with epithelial-mesenchymal transition (EMT), consolidation phase is mesenchymal-epithelial (MET). This dynamic referred as plasticity (EMP). While it known that EMP essential for cancer invasion metastatic spread, less about heterogeneity states within a tumor even between...
Abstract Merkel cell carcinoma (MCC) is a rare, highly aggressive skin cancer prevalent in elderly and immunocompromised patients. MCC immunogenic as it either associated with polyoma virus (MCPyV) integration or UV-associated mutations non-viral cases. Indeed, immune checkpoint inhibitors (CPI) like PD-1/PD-L1 blocking antibodies exert strong clinical activity; however, primary secondary resistance often occurs. Domatinostat (4SC-202) an orally available small molecule inhibitor targeting...
Merkel cell carcinoma (MCC) is a rare, highly aggressive skin cancer prevalent in elderly and immunocompromised patients. MCC immunogenic as it either associated with polyoma virus (MCPyV) integration or UV-associated mutations non-viral cases. Indeed, immune checkpoint inhibitors (CPI) like PD-1/PD-L1 blocking antibodies exert strong clinical activity; however, primary secondary resistance often occurs. Domatinostat (4SC-202) an orally available small molecule inhibitor targeting histone...