A5065585213- Pancreatic function and diabetes
- Traumatic Brain Injury and Neurovascular Disturbances
- Metabolism, Diabetes, and Cancer
- Traumatic Brain Injury Research
- Retinal Diseases and Treatments
- Retinal Imaging and Analysis
- Protein Kinase Regulation and GTPase Signaling
- Systemic Lupus Erythematosus Research
- Adipose Tissue and Metabolism
- Autophagy in Disease and Therapy
- Blood Pressure and Hypertension Studies
- Microbial Metabolic Engineering and Bioproduction
- S100 Proteins and Annexins
- Preterm Birth and Chorioamnionitis
- Advanced Neuroimaging Techniques and Applications
- Cardiovascular Health and Disease Prevention
- Endometriosis Research and Treatment
- Calcium signaling and nucleotide metabolism
- GDF15 and Related Biomarkers
- Healthcare and Venom Research
- Macrophage Migration Inhibitory Factor
- Molecular Communication and Nanonetworks
- Aldose Reductase and Taurine
- Child Abuse and Related Trauma
- Neuroblastoma Research and Treatments
Hallym University Kangnam Sacred Heart Hospital
2022-2025
Hallym University
2015-2025
Johns Hopkins Medicine
2014-2024
Johns Hopkins University
2014-2024
National Eye Institute
2023-2024
National Institutes of Health
2023-2024
Yonsei University
2011-2024
Hallym University Medical Center
2022
Seoul National University Bundang Hospital
2014-2022
Dong-Eui University
2007
Abstract Non-neovascular or dry age-related macular degeneration (AMD) is a multi-factorial disease with of the aging retinal-pigmented epithelium (RPE). Lysosomes play crucial role in RPE health via phagocytosis and autophagy, which are regulated by transcription factor EB/E3 (TFEB/E3). Here, we find that increased AKT2 inhibits PGC-1α to downregulate SIRT5, identify as an binding partner. Crosstalk between SIRT5 facilitates TFEB-dependent lysosomal function RPE. AKT2/SIRT5/TFEB pathway...
It has been known that O-linked β-N-acetylglucosamine (O-GlcNAc) modification of proteins plays an important role in transcription, translation, nuclear transport and signal transduction. The increased flux glucose through the hexosamine biosynthetic pathway (HBP) O-GlcNAc protein have suggested as one causes development insulin resistance. However, it is not clear at molecular level, how results substantial impairment signaling. To clarify association resistance rat primary adipocytes, we...
Although the majority of Alzheimer's disease (AD) cases are sporadic, about 5% inherited in an autosomal dominant pattern as familial AD (FAD) and manifest at early age. Mutations presenilin 1 (PSEN1) gene account for early-onset FAD. Here, we describe generation virus-free human induced pluripotent stem cells (hiPSCs) derived from fibroblasts patients harboring FAD PSEN1 mutation A246E healthy age-matched controls using nonintegrating episomal vectors. We have differentiated these hiPSC...
Blast injury to brain, a hundred-year old problem with poorly characterized neuropathology, has resurfaced as health concern in recent deployments Iraq and Afghanistan. To characterize the neuropathology of blast injury, we examined brains veterans for presence amyloid precursor protein (APP)-positive axonal swellings typical diffuse (DAI) compared them healthy controls well opiate overdose, anoxic-ischemic encephalopathy, non-blast TBI (falls motor vehicle crashes).In cases history, found...
Abstract Introduction Diffuse axonal injury is an extremely common type of traumatic brain encountered in motor vehicle crashes, sports injuries, and combat. Although many cases diffuse result chronic disability, there are no current treatments for this condition. Its basic lesion, injury, has been aggressively modeled primate rodent animal models. The inexorable perikaryal degeneration dysmyelination often calls regenerative therapies, including therapies based on stem cells precursors....
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder characterized by gradual degeneration and elimination of motor neurons (MNs) in the cortex spinal cord. Some familial forms ALS are caused genetic mutations superoxide dismutase 1 (SOD1) but mechanisms driving MN disease unclear. Identifying naturally occurring pathology understanding how this mutant SOD1 can affect MNs valid reliable human cell model remains to be established. Here, using CRISPR/Cas9 genome editing...
Mitochondrial dysfunction contributes to a number of human diseases, such as hyperlipidemia, obesity, and diabetes. The mutation reduction mitochondrial DNA (mtDNA) have been suggested factors in the pathogenesis To elucidate association cellular mtDNA content insulin resistance, we produced L6 GLUT4myc myocytes depleted by long term treatment with ethidium bromide. cells cultured 0.2 mug/ml bromide (termed cells) revealed marked decrease ATP content, concomitant lack mRNAs encoded mtDNA....
Abstract Background Traumatic brain injury (TBI) is a major cause of CNS neurodegeneration and has no disease-altering therapies. It commonly associated with specific type biomechanical disruption the axon called traumatic axonal (TAI), which often leads to sometimes perikaryal degeneration neurons. We have previously used genome-scale, arrayed RNA interference-based screens in primary mouse retinal ganglion cells (RGCs) identify pair related kinases, dual leucine zipper kinase (DLK) (LZK)...
Traumatic axonal injury (TAI) and the associated axonopathy are common consequences of traumatic brain (TBI) contribute to significant neurological morbidity. It has been previously suggested that TAI activates a highly conserved program self-destruction known as Wallerian degeneration (WD). In present study, we utilize our well-established impact acceleration model TBI (IA-TBI) characterize pathology injured myelinated axons in white matter tracks traversing ventral, lateral, dorsal spinal...
Traumatic axonal injury (TAI), thought to be caused by rotational acceleration of the head, is a prevalent neuropathology in traumatic brain (TBI). TAI optic nerve common finding multiple blunt-force TBI models and hence great model study mechanisms treatments for TAI, especially view compartmentalized anatomy visual system. We have previously shown that somata proximal, but not distal, axons retinal ganglion cells (RGC) respond DLK/LZK blockade after impact head (IA-TBI). Here, we explored...
Antimicrobial peptides, such as beta-defensin 2 (BD2), are vital in controlling infections and immune responses. In this study, we investigated the expression role of BD2 amniotic membrane human epithelial cells (hAECs) from patients with preterm birth chorioamnionitis, focusing on its regulation inflammatory cytokines protective effect barrier. Our results show increased Lipopolysaccharide (LPS)-induced inflammation release hAECs a dose- time-dependent manner. treatment effectively...
Wallerian degeneration (WD) is a conserved axonal self-destruction program implicated in several neurological diseases. WD driven by the degradation of NAD+ synthesizing enzyme NMNAT2, buildup its substrate NMN, and activation degrading SARM1, eventually leading to fragmentation. The regulation amenability these events therapeutic interventions remain unclear. Here we explored pharmacological strategies that modulate NMN metabolism, namely inhibition NMN-synthesizing NAMPT, nicotinic acid...
Insulin-induced GLUT4 recruitment to the plasma membrane involves trafficking through multiple subcellular compartments regulated by proteins, many of which are yet be identified. Here we describe a 65 kDa protein found in purified vesicles rat adipocytes as potential traffic regulatory protein. On basis MALDI-TOF MS, RT-PCR, gene cloning, sequencing, and immunoreactivity data, identified this EHD2, member EH domain-containing proteins that have been implicated vesicle trafficking. EHD2 was...
The Clinical Association of the Blood Pressure Variability with Target Organ Damage in Hypertensive Patients Chronic Kidney DiseaseIt is known that blood pressure variability (BPV) can independently affect target organ damage (TOD), even normal pressure.There have been few studieson chronic kidney disease (CKD) patients.We evaluated relationship between BPV and TOD a cross-sectional, multicenter study on hypertensive CKD 1,173 patients using 24-hr ambulatory monitoring.BPV was defined as...
Insulin recruits GLUT4 from an intracellular location to the plasma membrane in rat adipocytes. The process involves multiple compartments and protein functions, details of which are largely unknown partly due our inability separate individual compartments. Here, by hypotonic lysis, differential centrifugation, glycerol density gradient sedimentation, we separated adipocytes into three fractions: membrane-containing fraction T membrane-free fractions H L. contents T, H, L were approximately...
The trafficking kinetics of GLUT4 and GLUT1 in rat epididymal adipocytes were analyzed by a four-compartment model based upon steady-state pool sizes three intracellular fractions one plasma membrane fraction separated assessed under both basal insulin-stimulated states. compartment provided relative values the kinetic coefficients characterizing rate each process loop. Absolute these obtained matching simulated half-times to those observed experimentally reported literature for Our analysis...
Purpose: Temporomandibular disorder (TMD) is one of the most common diseases causing chronic orofacial pain. Prolotherapy called 'regenerative injection therapy' or 'growth factor stimulation injection', and it induces functional reactivation tissues such as ligaments tendons. The aim this study to evaluate efficacy ultrasound-guided prolotherapy with platelet-rich plasma (PRP) for patients who had TMD symptoms, especially in temporomandibular joint (TMJ) pain, restricted mouth opening, TMJ...
The retinal pigment epithelium (RPE) is a monolayer of hexagonal cells located at the back eye. It provides nourishment and support to photoreceptors choroidal capillaries, performs phagocytosis photoreceptor outer segments (POS), secretes cytokines in polarized manner for maintaining homeostasis retina. Dysfunctional RPE, caused by mutations, aging, environmental factors, results degeneration other layers causes vision loss. A hallmark phenotypic feature degenerating RPE intra sub-cellular...
Hysterectomy is commonly performed for benign gynecological diseases. Minimally invasive surgical approaches offer several advantages. Unfortunately, few studies have compared the outcomes of different types minimally surgeries. Therefore, this study aimed to compare robotic hysterectomy (RH) and conventional laparoscopic (CLH) in gynecologic We a retrospective cohort at single center between January 2014 July 2022. A total 397 patients (RH: 197 CLH: 200) who underwent diseases with uterine...