A5104728293- Traumatic Brain Injury Research
- Traumatic Brain Injury and Neurovascular Disturbances
- Neurogenesis and neuroplasticity mechanisms
- Nerve injury and regeneration
- Pluripotent Stem Cells Research
- Neurogenetic and Muscular Disorders Research
- S100 Proteins and Annexins
- Amyotrophic Lateral Sclerosis Research
- Advanced Neuroimaging Techniques and Applications
- Neuroscience and Neural Engineering
- Neuroinflammation and Neurodegeneration Mechanisms
- Mesenchymal stem cell research
- Cardiac Arrest and Resuscitation
- Spinal Cord Injury Research
- COVID-19 Clinical Research Studies
- Enzyme Catalysis and Immobilization
- Tissue Engineering and Regenerative Medicine
- Neuroblastoma Research and Treatments
- Urinary Bladder and Prostate Research
- Sleep and Wakefulness Research
- Prenatal Substance Exposure Effects
- Traditional Chinese Medicine Analysis
- Cerebrospinal fluid and hydrocephalus
- Child Abuse and Related Trauma
- Phosphodiesterase function and regulation
Zhejiang University
2023
Johns Hopkins Medicine
2008-2019
Johns Hopkins University
2007-2019
University of Baltimore
2016
Army Medical University
2014
Johns Hopkins University Applied Physics Laboratory
2011
Weatherford College
2008
University of Maryland, Baltimore
2004-2006
Nephrology Center of Maryland
2006
Peking University People's Hospital
2003
The increased use of explosives in recent wars has the number veterans with blast injuries. Of particular interest is injury to brain, and a key question whether primary overpressure wave injurious or brain from mostly due secondary tertiary effects. Using shock tube generating waves comparable open-field waves, we explored effects on parenchymatous organs mice emphasis brain. main injuries nonbrain were hemorrhages lung interstitium alveolar spaces hemorrhagic infarcts liver, spleen,...
Experimental therapeutics for degenerative and traumatic diseases of the nervous system have been recently enriched with addition neural stem cells (NSCs) as alternatives to fetal tissues cell replacement. Neurodegenerative present additional problem that death signals may interfere viability grafted cells. The adult spinal cord raises further challenges NSC differentiation because lack intrinsic developmental potential negative outcomes several prior attempts.NSCs from human were into...
Effective treatments for degenerative and traumatic diseases of the nervous system are not currently available. The support or replacement injured neurons with neural grafts, already an established approach in experimental therapeutics, has been recently invigorated addition embryonic stem-derived precursors as inexhaustible, self-propagating alternatives to fetal tissues. adult spinal cord, i.e., site common devastating injuries motor neuron disease, especially challenging target stem cell...
Cell replacement strategies for degenerative and traumatic diseases of the nervous system depend on functional integration grafted cells into host neural circuitry, a condition necessary propagation physiological signals and, perhaps, targeting trophic support to injured neurons. We have recently shown that human stem cell (NSC) grafts ameliorate motor neuron disease in SOD1 transgenic rodents. Here we study structural aspects neuronally differentiated NSCs circuitry G93A rats. Human were...
Amyotrophic lateral sclerosis (ALS) is a target for cell-replacement therapies, including therapies based on human neural stem cells (NSCs). These must be first tested in the appropriate animal models, transgenic rodents harboring superoxide dismutase (SOD1) mutations linked to familial ALS. However, these rodent subjects reject discordant xenografts. In present investigation, we grafted NSCs from embryonic spinal cord into ventral lumbar of 2-month-old SOD1-G93A mice. Animals were...
The potential of human embryonic stem (ES) cells as experimental therapies for neuronal replacement has recently received considerable attention. In view the organization mature nervous system into distinct neural circuits, key challenges such are directed differentiation ES cell-derived precursors (NPs) specific types and directional growth axons along specified trajectories. present study, we cultured NPs derived from NIH-approved line BGO1 on polycaprolactone fiber matrices different...
Blast injury to brain, a hundred-year old problem with poorly characterized neuropathology, has resurfaced as health concern in recent deployments Iraq and Afghanistan. To characterize the neuropathology of blast injury, we examined brains veterans for presence amyloid precursor protein (APP)-positive axonal swellings typical diffuse (DAI) compared them healthy controls well opiate overdose, anoxic-ischemic encephalopathy, non-blast TBI (falls motor vehicle crashes).In cases history, found...
Abstract Disrupted‐In‐Schizophrenia 1 (DISC1) is one of two genes that straddle the chromosome breakpoint a translocation associated with an increased risk schizophrenia. DISC1 has been identified in brain various mammalian species, but no previous immunocytochemical studies have conducted human neocortex. We examined immunoreactivity frontal and parietal cortex (BA 4, 9, 39, 46) normal brain. At light microscopic level, immunolabeling was prominent neuropil, multiple populations cells,...
Multiple missense mutations in Leucine–rich repeat kinase 2 (LRRK2) have been linked to Parkinson's disease (PD), the most common degenerative movement disorder. LRRK2 is expressed by both neurons and microglia, residential immune cells brain. Increasing evidence supports a role of modulating microglial activity, which Lrrk2-null rodent microglia display less inflammatory response endotoxin lipopolysaccharide (LPS). The underlying molecular mechanism, however, remains elusive. Chemokine...
Abstract Introduction Diffuse axonal injury is an extremely common type of traumatic brain encountered in motor vehicle crashes, sports injuries, and combat. Although many cases diffuse result chronic disability, there are no current treatments for this condition. Its basic lesion, injury, has been aggressively modeled primate rodent animal models. The inexorable perikaryal degeneration dysmyelination often calls regenerative therapies, including therapies based on stem cells precursors....
Abstract Stem cell grafts have been advocated as experimental treatments for neurological diseases by virtue of their ability to offer trophic support injured neurons and, theoretically, replace dead neurons. Human embryonic stem cells (HESCs) are a rich source neural precursors (NPs) grafting, but questioned tendency form tumors. Here we studied the HESC-derived NP optimized number and differentiation stage prior transplantation, survive stably differentiate integrate in basal forebrain...
Abstract Background Traumatic brain injury (TBI) is a major cause of CNS neurodegeneration and has no disease-altering therapies. It commonly associated with specific type biomechanical disruption the axon called traumatic axonal (TAI), which often leads to sometimes perikaryal degeneration neurons. We have previously used genome-scale, arrayed RNA interference-based screens in primary mouse retinal ganglion cells (RGCs) identify pair related kinases, dual leucine zipper kinase (DLK) (LZK)...
Reelin is a glycoprotein that plays critical role in brain development, including proper cortical lamination. In adult animals, reelin continues to be expressed different neuronal populations many regions. We performed labeling for immunoreactivity (-i) post-mortem cerebral cortex from five adults and two fetuses with three antibodies. The tissue was then processed light electron microscopy. cell bodies, reelin-i found pyramidal nonpyramidal neurons on the outer nuclear membrane, rough...
Aim: To explore the hypothesis that grafts of exogenous stem cells in spinal cord athymic rats or with transgenic motor neuron disease can induce endogenous and initiate intrinsic repair mechanisms be exploited amyotrophic lateral sclerosis therapeutics. Materials & methods: Human neural (NSCs) were transplanted into lower lumbar healthy to whether signals related normal subjects. Patterns migration differentiation NSCs gray white matter, emphasis on central canal region ependymal...
Enzymatic reduction of diphenylmethanimine derivatives has rarely been reported owing to their steric hindrance. Herein, imine reductase (IRED) from Nocardia cyriacigeorgica rationally engineered with an efficient strategy focused rational iterative site-specific mutagenesis (FRISM) was selected for the a series N-cyclopropylmethyl-1-aryl-1-phenylmethylimines. Two highly enantioselective IRED variants were identified, providing various bulky amine products moderate high yields and ee values...