A5011654140- Respiratory Support and Mechanisms
- Neonatal Respiratory Health Research
- Respiratory viral infections research
- Sepsis Diagnosis and Treatment
- Infection Control and Ventilation
- Congenital Diaphragmatic Hernia Studies
- COVID-19 epidemiological studies
- Inhalation and Respiratory Drug Delivery
- Cardiac Arrest and Resuscitation
- Pneumonia and Respiratory Infections
- Airway Management and Intubation Techniques
- Neuroscience of respiration and sleep
- Tracheal and airway disorders
- Mechanical Circulatory Support Devices
- COVID-19 impact on air quality
- Renal function and acid-base balance
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Hemodynamic Monitoring and Therapy
- Phonocardiography and Auscultation Techniques
- Family and Patient Care in Intensive Care Units
- Immune Response and Inflammation
- Restraint-Related Deaths
- High Altitude and Hypoxia
- Electrolyte and hormonal disorders
Emma Kinderziekenhuis
2016-2025
Amsterdam University Medical Centers
2018-2025
University of Amsterdam
2009-2024
Amsterdam UMC Location University of Amsterdam
2011-2024
Semey Medical University
2023
Northern State Medical University
2020
Radboud University Nijmegen
2019
Radboud University Medical Center
2019
University Medical Center
2019
Boston Children's Hospital
2015
Human respiratory syncytial virus (RSV) is the most important cause of severe lower tract disease (LRTD) in young children worldwide. Extensive neutrophil accumulation lungs and occlusion small airways by DNA-rich mucus plugs are characteristic features RSV-LRTD. Activated neutrophils can release extracellular traps (NETs), networks DNA covered with antimicrobial proteins, as part first-line defence against pathogens. NETs trap eliminate microbes; however, abundant NET formation may also...
Transmission of SARS-CoV-2 leading to COVID-19 occurs through exhaled respiratory droplets from infected humans. Currently, however, there is much controversy over whether aerosol microdroplets play an important role as a route transmission. By measuring and modeling the dynamics droplets, we can assess relative contribution aerosols spreading SARS-CoV-2. We measure size distribution, total numbers, volumes including aerosols, by speaking coughing healthy subjects. Dynamic allows us account...
Viral bronchiolitis is the most common cause of respiratory failure requiring invasive ventilation in young children. Bacterial co-infections may complicate and prolong paediatric intensive care unit (PICU) stay. Data on prevalence, type pathogens its association with disease severity are limited though. These data especially important as bacterial be treated using antibiotics could reduce duration PICU We investigated prevalence co-infection stay.Retrospective cohort study ventilated...
Abstract Nebulization of mRNA therapeutics can be used to directly target the respiratory tract. A promising prospect is that mucosal administration lipid nanoparticle (LNP)-based vaccines may lead a more efficient protection against viruses. However, nebulization process rupture LNP vehicles and degrade molecules inside. Here we present novel method able preserve substantially integrity vaccines, as tested with two SARS-CoV-2 vaccines. We compare new well-known methods for medical...
Objective: Angiotensin-converting enzyme and its effector peptide angiotensin II have been implicated in the pathogenesis of acute respiratory distress syndrome. Recently, angiotensin-converting 2 was identified as counter-regulatory that converts into angiotensin-(1-7). The aim this study to determine pulmonary activity patients with Design: Prospective observational pilot study. Setting: A PICU a university hospital. Patients: Fourteen admitted, requiring mechanical ventilation for...
Neutrophils are the predominant inflammatory cells recruited to respiratory tract as part of innate immune response viral infections. Recent reports indicate existence distinct functional neutrophil subsets in circulatory compartment adults, following severe conditions. Here, we evaluated occurrence blood and broncho-alveolar lavage fluid during infection infants based on CD16/CD62L expression. We show that course may develop four heterogeneous (mature, immature, progenitor, suppressive...
Viral-lower respiratory tract disease is common in young children worldwide and associated with high morbidity. Acute failure due to viral-lower necessitates PICU admission for mechanical ventilation. In critically ill patients settings, early fluid overload adverse outcomes such as prolonged ventilation increased mortality. It unclear, however, if this also applies induced acute failure. study, we aimed investigate the relation of mechanically ventilated a retrospective...
We measure aerosol persistence to assess the risk of transmission severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in public spaces. Direct measurement concentrations, however, has proven be technically difficult; we propose use handheld particle counters as a novel and easily applicable method concentrations. This allows us perform measurements typical spaces, each differing volume, number people, ventilation rate. These data are used estimate relation between time infection...
Viral bronchiolitis is a major cause of pediatric intensive care unit (PICU) admission. Insight in the trends bronchiolitis-associated PICU admissions limited, but imperative for future resource and capacity planning.We retrospectively studied six European sites, including three full national registries, between 2000 2019 calculated population-based estimates per 100,000 children where appropriate. Information concerning risk factors severe disease use invasive mechanical ventilation was...
There is a worldwide discussion on the role of aerosols in transmission SARS-CoV-2. The WHO has recently highlighted1 possible Aerosols containing small respirable particles are increasingly recognized as potentially important mode SARS-CoV-2.2-5 We investigate aerosol droplet persistence inside confined space hospital elevators and propose specific measures to reduce associated risk SARS-CoV-2 transmission. To elevators, we mimic single cough using specially designed spray nozzle disperse...
Mucus obstruction is a key feature of many inflammatory airway diseases. Neutrophil extracellular traps (NETs) are released upon neutrophil stimulation and consist chromatin networks studded with cytotoxic proteins. When in the airways, these NETs can become part mucus. We hypothesized that DNA and/or oxidative stress (e.g., by release reactive oxygen species myeloperoxidase during formation airways) would increase mucus viscoelasticity. collected human from endotracheal tubes healthy...
Respiratory syncytial virus (RSV) bronchiolitis causes substantial morbidity and mortality in young children, but insight into the burden of RSV on pediatric intensive care units (PICUs) is limited. We aimed to determine PICUs Netherlands. Therefore, we identified all children ≤ 24 months age with between 2003 2016 from a nationwide PICU registry. Subsequently manually checked their patient records for correct diagnosis collected characteristics, additional clinical data, respiratory support...
Lower respiratory tract infection by syncytial virus (RSV) is a frequent cause of acute lung injury in young children and infants. Studies adults animals suggest that tumor necrosis factor receptor (TNFR) ligands may mediate causing apoptosis epithelial cells. The main goal the present study was to determine whether TNF-related apoptosis-inducing ligand (Apo2L/TRAIL) pathway be implicated during severe RSV children. We report elevated levels soluble (s)TRAIL released leukocytes...
There is a persistent concern over the risk of respiratory pathogen transmission, including SARS-CoV-2, via formation aerosols (ie, suspension microdroplets and residual microparticles after evaporation) generated during high-flow nasal cannula (HFNC) oxygen therapy in critically ill patients. This fueled by limited available studies on this subject. In study, we tested our hypothesis that HFNC treatment not associated with increased aerosol as compared to conventional therapy.We used laser...
Appropriate fluid management in mechanically ventilated critically ill children remains an important challenge and topic of active discussion pediatric intensive care medicine. An increasing number studies show association between a positive balance or overload adverse outcomes. However, to date, no international consensus regarding removal strategies exists. The aim this study was obtain more insight into the current clinical practice therapy children. On behalf section cardiovascular...