A5048444823- Silymarin and Mushroom Poisoning
- Berberine and alkaloids research
- Synthesis and bioactivity of alkaloids
- Polyamine Metabolism and Applications
- Cancer, Lipids, and Metabolism
- Pesticide Exposure and Toxicity
- Genomics, phytochemicals, and oxidative stress
- Cancer-related Molecular Pathways
- Tannin, Tannase and Anticancer Activities
- Cancer, Hypoxia, and Metabolism
- Phytase and its Applications
- Selenium in Biological Systems
- Phytochemicals and Antioxidant Activities
- Drug-Induced Hepatotoxicity and Protection
- Tea Polyphenols and Effects
- Retinoids in leukemia and cellular processes
- Toxin Mechanisms and Immunotoxins
- Heme Oxygenase-1 and Carbon Monoxide
- Skin Protection and Aging
- Metabolomics and Mass Spectrometry Studies
- Flavonoids in Medical Research
- Natural product bioactivities and synthesis
- Redox biology and oxidative stress
- Prostate Cancer Treatment and Research
- Sirtuins and Resveratrol in Medicine
University of Montana
2015-2025
University of Colorado Anschutz Medical Campus
2016-2025
University of Colorado Denver
2015-2024
University of Colorado Cancer Center
2015-2024
University of Colorado Health
2002-2023
Indian Veterinary Research Institute
2010-2023
M.J.P. Rohilkhand University
2020-2022
Oil and Natural Gas Corporation (India)
2022
Subharti Medical College
2021
University of Colorado System
2002-2020
Hypoxic conditions in prostate cancer (PCA) are associated with poor prognosis; however, precise mechanism/s through which hypoxia promotes malignant phenotype remains unclear. Here, we analyzed the role of exosomes from hypoxic PCA cells enhancing invasiveness and stemness naïve cells, as well promoting cancer-associated fibroblast (CAF) stromal (PrSC). Human LNCaP PC3 were exposed to (1% O2) or normoxic (21% conditions, secreted under (ExoHypoxic) (ExoNormoxic) isolated conditioned media....
Procyanidins present in grape seeds are known to exert anti-inflammatory, anti-arthritic and anti-allergic activities, prevent skin aging, scavenge oxygen free radicals inhibit UV radiation-induced peroxidation activity. Since most of these events associated with the tumor promotion stage carcinogenesis, studies suggest that seed polyphenols procyanidins therein could be anticarcinogenic and/or anti-tumor-promoting agents. Therefore, we assessed effect a polyphenolic fraction isolated from...
Reduction in serum prostate-specific antigen (PSA) levels has been proposed as an endpoint biomarker for hormone-refractory human prostate cancer intervention. We examined whether a flavonoid antioxidant silibinin (an active constituent of milk thistle) decreases PSA carcinoma LNCaP cells and this effect biological relevance. Silibinin treatment grown resulted significant decrease both intracellular secreted forms concomitant with highly to complete inhibition cell growth via G 1 arrest...
Abstract Extracts from the seeds of milk thistle, Silybum marianum, are known commonly as silibinin and silymarin possess anticancer actions on human prostate carcinoma in vitro vivo. Seven distinct flavonolignan compounds a flavonoid have been isolated commercial extracts. Most notably, two pairs diastereomers, silybin A B isosilybin B, among these compounds. In contrast, is composed only 1:1 mixture B. With isomers now quantities sufficient for biological studies, each pure compound was...
Abstract We isolated a coumarin compound decursin (C19H20O5; molecular weight 328) from Korean angelica (Angelica gigas) root and characterized it by spectroscopy. Here, for the first time, we observed that (25-100 μmol/L) treatment 24 to 96 hours strongly inhibits growth induces death in human prostate carcinoma DU145, PC-3, LNCaP cells. Furthermore, decursinol [where (CH3)2-C=CH-COO- side chain of is substituted with -OH] has much lower effects compared decursin, suggesting possible...
The anti-cancer efficacy of grape seed extract (GSE) against prostate cancer (PCA) via its anti-proliferative, pro-apoptotic and anti-angiogenic activities in both cell culture animal models have recently been described by us. GSE is a complex mixture containing gallic acid (GA), catechin (C), epicatechin (EC) several oligomers (procyanidins) C and/or EC, some which are esterified to GA. To determine components most active PCA, an ethyl acetate was separated reverse-phase high-performance...
Abstract BACKGROUND Silibinin is a polyphenolic flavonolignan derived from milk thistle ( Silybum marianium ) with anti‐oxidant properties. The purpose of the current trial was to determine tissue and blood effects high‐dose silybin‐phytosome in prostate cancer patients. METHODS Subjects localized planning for prostatectomy were eligible enroll. Six patients received 13 g daily six additional participants serving as control subjects. RESULTS Patients treatment arm 14–31 days (mean 20 days)...
Abstract Herein, for the first time, we investigated in vivo efficacy and associated molecular biomarkers mechanisms of a chemopreventive agent, silibinin, against human colorectal carcinoma (CRC) HT29 xenograft growth. Nude mice were implanted with cells fed vehicle (carboxymethyl cellulose or phosphatidylcholine) 200 mg/kg/d dose silibinin 100 doses silybin-phytosome (5 days per week) 32 days. Silibinin inhibited tumor growth that accounted 48% (P = 0.002) decrease volume 42% 0.012) weight...
Abstract Carcinogenesis is a multistage process consisting of initiation, promotion, and progression phases. Thus, the sequence events has many phases for prevention intervention. Chemoprevention, novel approach controlling cancer, involves use specific natural products or synthetic chemical agents to reverse, suppress, prevent premalignancy before development invasive cancer. Several including grains, nuts, cereals, spices, fruits, vegetables, beverages, medicinal plants, herbs, their...
Abstract Purpose: Accumulating evidences suggest the beneficial effects of fruit-and-vegetable consumption in lowering risk various cancers, including colorectal cancer. Herein, we investigated vitro and vivo anticancer associated mechanisms grape seed extract (GSE), a rich source proanthocyanidins, against Experimental Design: Effects GSE were examined on human cancer HT29 LoVo cells culture for proliferation, cell cycle progression, apoptosis. The effect oral was tumor xenograft growth...
The chronic nature of prostate cancer growth and progression leading to metastasis provides a large window for intervention. Herein, the first time, we investigated effect associated mechanisms silibinin phosphatidylcholine (silybin-phytosome) on established tumors in transgenic adenocarcinoma mouse (TRAMP) model.Twenty-week-old TRAMP male mice having palpable tumor were fed with control or 0.5% 1%, w/w, silybin-phytosome diets 11 weeks then sacrificed.Dietary inhibited (up 60%, P < 0.001)...
Silibinin, a flavanone from milk thistle, inhibits the growth of tumors in several rodent models. We examined effects dietary silibinin on growth, progression, and angiogenesis urethane-induced lung mice.A/J mice (15 per group) were injected with urethane (1 mg/g body weight) or saline alone fed normal diets for 2 weeks, after which they containing different doses (0%-1% [wt/wt] silibinin) 18 27 weeks. Immunohistochemistry Western blot analysis used to examine enzymatic markers inflammation,...