A5031669192- 3D Printing in Biomedical Research
- Microfluidic and Capillary Electrophoresis Applications
- Acoustic Wave Resonator Technologies
- Neuroscience and Neural Engineering
- Innovative Microfluidic and Catalytic Techniques Innovation
- Analytical Chemistry and Sensors
- Mechanical and Optical Resonators
- Advanced MEMS and NEMS Technologies
- Supercapacitor Materials and Fabrication
- Biosensors and Analytical Detection
- Advanced Biosensing Techniques and Applications
- Advanced Sensor and Energy Harvesting Materials
- Monoclonal and Polyclonal Antibodies Research
- Silicon Nanostructures and Photoluminescence
- Advanced Fluorescence Microscopy Techniques
- Pluripotent Stem Cells Research
- Ultrasonics and Acoustic Wave Propagation
- Luminescence and Fluorescent Materials
- MXene and MAX Phase Materials
- Sensor Technology and Measurement Systems
- Optical Coatings and Gratings
- bioluminescence and chemiluminescence research
- Plasmonic and Surface Plasmon Research
- Power Transformer Diagnostics and Insulation
- Microfluidic and Bio-sensing Technologies
Tampere University
2016-2025
VTT Technical Research Centre of Finland
1997-2016
Today's supercapacitor energy storages are typically discrete devices aimed for printed boards and power applications. The development of autonomous sensor networks wearable electronics the miniaturisation mobile would benefit substantially from solutions in which storage is integrated with active device. Nanostructures based on porous silicon (PS) provide a route towards integration due to very high inherent surface area volume ratio compatibility microelectronics fabrication processes....
Abstract Liver zonation is a fundamental characteristic of hepatocyte spatial heterogeneity, which challenging to recapitulate in traditional cell cultures. This study presents novel microfluidic device designed induce liver cultures by establishing an oxygen gradient using standard laboratory gases. The consists two layers; bottom layer containing gas channel network that delivers high (cell incubator air, 19% oxygen) and low oxygenated (nitrogen) gases create three distinct zones within...
An electromechanical film can be manufactured with different thickness and elasticity. The for sensor actuator applications is typically between 30 70 /spl mu/m. modeled using a simplified structure, which shows the reciprocity of operation models. value transducer constant depends on ambient temperature increases at higher temperatures. During aging begins to decrease permanently 323 K, but some sensitivity remains even 333 K. Corona charging that gives sim/200 pCN/sup -1/ HS01 type...
Abstract Ischemic heart disease is a major cause of death worldwide, and the only available therapy to salvage tissue reperfusion, which can initially further damage. Many therapeutics that have been promising in animal models failed human trials. Thus, functional based cardiac ischemia are required. In this study, induced pluripotent stem cell derived-cardiomyocyte (hiPSC-CM)-based platform for modeling ischemia–reperfusion was developed utilizing system enabling precise control over oxygen...
Developing bone replacement scaffolds has been a driving ambition of regenerative medicine. Although great progress achieved for small scaffolds, the real clinical need is large >5 mm. Oxygenating these challenging, as slow diffusion rates lead to necrotic regions in scaffold core. In this work, we modulate vitro oxygen concentration flow chamber using an external perfusion pump while imaging concentrations below scaffolds. With no flow, yeast cells growing deplete oxygen, especially from...
Cell culture in-vitro is a well-known method to develop cell and disease models for studying physiologically relevant mechanisms responses various applications in life sciences. Conventional methods instance, using static flasks or well plates, have limitations, as these cannot provide accurate tractable advanced studies. However, microscale systems can overcome this since they mimic the cells' natural microenvironment adequately. We developed portable live-cell imaging system with an...
The ElectroMechanical Film (EMFi) is a thin, cellular, biaxially oriented polypropylene film coated with metal electrodes. Elementary models one air gap structure have been previously proposed to describe the EMFi's operation as sensor and an actuator. In this work model based on multiple presented shown reduce into gap. are charge layers polypropylene/air interfaces film's surfaces. evaluated some experimental results.
Cell culture in stem cell research is on the rise, not only for basic but also its potential medical and therapeutic applications. Monitoring process using sensors throughout helps to optimize conditions optimal growth maximize yield from cells. Further, sensor data gives insight into developing better systems. Typical platforms, e.g. well plates or flasks, cannot be easily equipped with impose many challenges periodic measurements a CO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML"...
Ischemic heart disease is the most common cardiovascular and a major burden for healthcare worldwide. However, its pathophysiology still not fully understood, human-based models mechanisms treatments are needed. Here, we used human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) to model acute ischemia-reperfusion in our novel cell culture assembly. The assembly enables exchange of oxygen partial pressure cells within minutes, mimicking ischemic event. In this study, hypoxia...
Abstract Hypoxia is a condition where tissue oxygen levels fall below normal levels. In locally induced hypoxia due to blood vessel blockage, delivery becomes compromised. The site flow diminished the most forms zero-oxygen core, and different oxygenation zones form around this core with varying concentrations. Naturally, these differing microenvironments drive cells respond according their status. To study cellular processes in laboratory settings, gas should be controlled rapidly...
Luminescence-based oxygen sensing is a widely used tool in cell culture applications. In typical configuration, the luminescent indicators are embedded solid, oxygen-permeable matrix contact with medium. However, sensitive cultures even minimal leaching of potentially cytotoxic can become an issue. One way to prevent immobilize covalently into supporting matrix. this paper, we report on method where platinum(II)-5,10,15,20-tetrakis-(2,3,4,5,6-pentafluorphenyl)-porphyrin (PtTFPP) immobilized...
Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) provide a great resource for functional cell and tissue models that can be applied in heart research, pharmaceutical industry, future regenerative medicine. During model experiments, precise control of environmental parameters is important. Temperature fundamental parameter, the acute effect temperature on hiPSC-CM functions has previously been studied. This paper reports long-term, systematic response studies cultures....
Liver zonation is a fundamental characteristic of hepatocyte spatial heterogeneity, which challenging to recapitulate in traditional cell cultures. This study presents novel microfluidic device designed induce liver cultures by establishing an oxygen gradient using standard laboratory gases. The consists two layers; bottom layer containing gas channel network that delivers high and low oxygenated gases create three distinct zones within the culture chamber above. Computational simulations...
A phase fluorimetric sensor targeted for the monitoring of dissolved oxygen concentration in microfluidic vitro cell models is presented. The sensing surface consists sensitive fluorescent dyes (PtOEPK) embedded a thin polystyrene film. simulated fluorescence emission characteristics show highly anisotropic distribution, and an efficient optical read-out based on parabolic lens Experimental results that applied scheme allows one to use films (< 500 nm), dilute dye-polymer ratios (0.025%),...
VTT Technical Research Centre of Finland has developed two reader prototypes for immunodiagnostic tests. also a one-step, homogeneous noncompetitive immunoassay small analytes using recombinant antibodies and morphine as the model analyte. lateral flow test. Lateral test is strip, which sample area detection area. In there are attached to gold or fluorescence particles, captured into area, if desired The concentration measured then calculated from color change. second prototype based on Time...
VTT Technical Research Centre of Finland has developed two reader prototypes for immunodiagnostic tests. also a one-step, homogeneous noncompetitive immunoassay small analytes using recombinant antibodies and morphine as the model analyte.VTT lateral flow test. Lateral test is strip, which sample area detection area. In there are attached to gold or fluorescence particles, captured into area, if desired analyte. The concentration measured then calculated from color change.The second...