A5045325794- Microfluidic and Bio-sensing Technologies
- Cell Image Analysis Techniques
- 3D Printing in Biomedical Research
- Microfluidic and Capillary Electrophoresis Applications
- Gene Regulatory Network Analysis
- Fungal and yeast genetics research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Digital Holography and Microscopy
- Orbital Angular Momentum in Optics
- Nonlinear Dynamics and Pattern Formation
- Image Processing Techniques and Applications
- Microbial Metabolic Engineering and Bioproduction
- Pancreatic function and diabetes
- Advanced Fluorescence Microscopy Techniques
- Photoreceptor and optogenetics research
- Viral Infectious Diseases and Gene Expression in Insects
- Advanced biosensing and bioanalysis techniques
- AI in cancer detection
- Advanced Biosensing Techniques and Applications
- Plant Reproductive Biology
- Plant and Biological Electrophysiology Studies
- Lipid metabolism and biosynthesis
- CRISPR and Genetic Engineering
- Molecular Communication and Nanonetworks
- Single-cell and spatial transcriptomics
University of Gothenburg
2016-2025
In-Q-Tel
2011
Sahlgrenska University Hospital
2005
Lung Institute
2001
Abstract Optical tweezers are tools made of light that enable contactless pushing, trapping, and manipulation objects, ranging from atoms to space sails. Since the pioneering work by Arthur Ashkin in 1970s, optical have evolved into sophisticated instruments been employed a broad range applications life sciences, physics, engineering. These include accurate force torque measurement at femtonewton level, microrheology complex fluids, single micro- nano-particle spectroscopy, single-cell...
The design and fabrication of a very large-scale liver-lobule (VLSLL)-on-a-chip device, providing microphysiological niche for hepatocytes, is described. device consists an integrated network liver-lobule-like hexagonal tissue-culture chambers constructed in hybrid layout with separate seed-feed network. As key feature, each chamber contains central outlet mimicking the vein liver lobule. Separating walls located between culture area feed protects cells from shear force convective flow....
Adaptation to altered osmotic conditions is a fundamental property of living cells and has been studied in detail the yeast Saccharomyces cerevisiae. Yeast accumulate glycerol as compatible solute, controlled at different levels by High Osmolarity Glycerol (HOG) response pathway. Up now, essentially all osmostress studies have performed with glucose carbon energy source, which metabolised glycolysis by-product. Here we investigated stress when respiring ethanol source. Remarkably, do not...
Yeast glycolytic oscillations have been studied since the 1950s in cell-free extracts and intact cells. For cells, sustained so far only observed at population level, i.e. for synchronized cultures high biomass concentrations. Using optical tweezers to position yeast cells a microfluidic chamber, we were able observe individual isolated detailed kinetic model cellular reactions, simulated heterogeneity response of assuming small differences single internal parameter. This is first time that...
Signal transmission progresses via a series of transient protein-protein interactions and protein movements, which require diffusion within cell packed with different molecules. Yeast Hog1, the effector kinase High Osmolarity Glycerol pathway, translocates transiently from cytosol to nucleus during adaptation high external osmolarity. We followed dynamics osmostress-induced volume loss Hog1 nuclear accumulation upon exposure cells NaCl concentrations. While peaked five minutes following mild...
Characterization of suspended nanoparticles in their native environment plays a central role wide range fields, from medical diagnostics and nanoparticle-enhanced drug delivery to nanosafety environmental nanopollution assessment. Standard optical approaches for nanoparticle sizing assess the size via diffusion constant and, as consequence, require long trajectories that medium has known uniform viscosity. However, most biological applications, only short are available, while simultaneously,...
Quantitative analysis of cell structures is essential for biomedical and pharmaceutical research. The standard imaging approach relies on fluorescence microscopy, where interest are labeled by chemical staining techniques. However, these techniques often invasive sometimes even toxic to the cells, in addition being time consuming, labor intensive, expensive. Here, we introduce an alternative deep-learning–powered based bright-field images a conditional generative adversarial neural network...
Oscillations are widely distributed in nature and synchronization of oscillators has been described at the cellular level (e.g. heart cells) population fireflies). Yeast glycolysis is best known oscillatory system, although it studied almost exclusively (i.e. limited to observations average behaviour synchronized cultures). We individual yeast cells that were positioned with optical tweezers a microfluidic chamber determine precise conditions for autonomous glycolytic oscillations. Hopf...
Cell volume is a biophysical property, which of great importance for quantitative characterisations biological processes, such as osmotic adaptation. It also crucial parameter in the most common type mathematical description cellular behaviour-ordinary differential equation (ODE) models, e.g. integrative model stress response baker's yeast (E. Klipp, B. Nordlander, R. Kruger, P. Gennemark and S. Hohmann, Nat. Biotechnol., 2005, 23, 975-982). Until recently only rough estimates this value...
Abstract Cell signaling, gene expression and metabolism are affected by cell-cell heterogeneity random changes in the environment. The effects of such fluctuations on cell signaling have recently been studied intensively using single-cell experiments. In may be particularly important because it affect synchronisation metabolic oscillations, an example communication. This is notoriously difficult to describe theoretically as glycolytic oscillations shows: neither mechanism understood nor role...
The last decade has seen a rapid development of experimental techniques that allow data collection from individual cells. These have enabled the discovery and characterization variability within population genetically identical Nonlinear mixed effects (NLME) modeling is an established framework for studying between individuals in population, frequently used pharmacokinetics pharmacodynamics, but its potential studies cell-to-cell molecular cell biology yet to be exploited. Here we take...
Recent advancements in deep learning (DL) have propelled the virtual transformation of microscopy images across optical modalities, enabling unprecedented multimodal imaging analysis hitherto impossible. Despite these strides, integration such algorithms into scientists' daily routines and clinical trials remains limited, largely due to a lack recognition within their respective fields plethora available methods. To address this, we present structured overview cross-modality transformations,...
Abstract Liver zonation is a fundamental characteristic of hepatocyte spatial heterogeneity, which challenging to recapitulate in traditional cell cultures. This study presents novel microfluidic device designed induce liver cultures by establishing an oxygen gradient using standard laboratory gases. The consists two layers; bottom layer containing gas channel network that delivers high (cell incubator air, 19% oxygen) and low oxygenated (nitrogen) gases create three distinct zones within...
In vitro cell culture models play a crucial role in preclinical drug discovery. To achieve optimal culturing environments and establish physiologically relevant organ-specific conditions, it is imperative to replicate in...
In this study, we tested the hypothesis that two separate pathways, two-step process and an apolipoprotein B (apoB) size-dependent lipidation process, give rise to different lipoproteins. Expression of apoB-100 C-terminally truncated forms in McA-RH7777 cells demonstrated VLDL particles can be assembled by apoB linear lipidation, resulting whose density is inversely related size apoB. This results a LDL-VLDL 2 particle containing apoB-100. 1 apoB-48 larger but not any significant amount...
The possibility to conduct complete cell assays under a precisely controlled environment while consuming minor amounts of chemicals and precious drugs have made microfluidics an interesting candidate for quantitative single-cell studies. Here, we present application-specific microfluidic device, cellcomb, capable conducting high-throughput experiments. system employs pure hydrodynamic forces easy trapping is readily fabricated in polydimethylsiloxane (PDMS) using soft lithography techniques....
The yeast AMPK/SNF1 pathway is best known for its role in glucose de/repression. When becomes limited, the Snf1 kinase activated and phosphorylates transcriptional repressor Mig1, which then exported from nucleus. exact mechanism how Snf1-Mig1 regulated not entirely elucidated. Glucose uptake through low affinity transporter Hxt1 results nuclear accumulation of Mig1 response to all concentrations upshift, however with increasing concentration localization more intense. Strains expressing...
Metabolic oscillations in single cells underlie the mechanisms behind cell synchronization and cell-cell communication. For example, glycolytic mediated by biochemical communication between may synchronize pulsatile insulin secretion pancreatic tissue, a link anomalies type-2 diabetes has been hypotesized. Cultures of yeast have provided an ideal model system to study propagation waves large populations. However, mechanism which occurs at individual level overcome local chemical...
Through digital imaging, microscopy has evolved from primarily being a means for visual observation of life at the micro- and nano-scale, to quantitative tool with ever-increasing resolution throughput. Artificial intelligence, deep neural networks, machine learning are all niche terms describing computational methods that have gained pivotal role in microscopy-based research over past decade. This Roadmap is written collectively by prominent researchers encompasses selected aspects how...
Epigallocatechin gallate (EGCG) increases the formation of cytosolic lipid droplets by a mechanism that is independent rate triglyceride biosynthesis and involves an enhanced fusion between droplets, process crucial for their growth in size. EGCG treatment reduced secretion both triglycerides apolipoprotein B-100 (apoB-100) VLDLs but not transferrin, albumin, or total proteins, indicating diverts from VLDL assembly to storage cytosol. This further supported observed increase intracellular...