A5030425945- Cell Image Analysis Techniques
- Virus-based gene therapy research
- Image Processing Techniques and Applications
- Herpesvirus Infections and Treatments
- Digital Imaging for Blood Diseases
- Poxvirus research and outbreaks
- AI in cancer detection
- Advanced Fluorescence Microscopy Techniques
- COVID-19 diagnosis using AI
- Bacteriophages and microbial interactions
- Diabetic Foot Ulcer Assessment and Management
- Cytomegalovirus and herpesvirus research
- Data-Driven Disease Surveillance
- Animal Virus Infections Studies
- Advanced X-ray Imaging Techniques
- Toxoplasma gondii Research Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Viral Infections and Immunology Research
- CRISPR and Genetic Engineering
- Mental Health via Writing
- Radiomics and Machine Learning in Medical Imaging
- Systemic Lupus Erythematosus Research
- interferon and immune responses
University of Wrocław
2023-2025
Institute of Computer Science
2024-2025
Helmholtz-Zentrum Dresden-Rossendorf
2022-2025
Center for Advanced Systems Understanding
2022-2025
MRC Laboratory for Molecular Cell Biology
2017-2024
University College London
2017-2024
The Royal Free Hospital
2022-2024
Roche Pharma AG (Germany)
2022-2023
Roche (United Kingdom)
2021-2022
University of Zurich
2012-2021
For image-based infection biology, accurate unbiased quantification of host–pathogen interactions is essential, yet often performed manually or using limited enumeration employing simple image analysis algorithms based on segmentation. Host protein recruitment to pathogens refractory automated assessment due its heterogeneous nature. An intuitive intelligent program assess host within general cellular pathogen defense lacking. We present HRMAn (Host Response Microbe Analysis), an open-source...
ABSTRACT Viruses spread between cells, tissues, and organisms by cell-free cell-cell transmissions. Both mechanisms enhance disease development, but it is difficult to distinguish them. Here, we analyzed the transmission mode of human adenovirus (HAdV) in monolayers epithelial cells wet laboratory experimentation a computer simulation. Using live-cell fluorescence microscopy replication-competent HAdV2 expressing green fluorescent protein, found that infection invariably occurred after cell...
Classical plaque assay measures the propagation of infectious agents across a monolayer cells. It is dependent on cell lysis, and limited by user-specific settings low throughput. Here, we developed Plaque2.0, broadly applicable, fluorescence microscopy-based high-throughput method to mine patho-biological clonal features. Plaque2.0 an open source framework extract information from chemically fixed cells immuno-histochemistry or RNA in situ hybridization, live expressing GFP transgene....
Transport of large cargo through the cytoplasm requires motor proteins and polarized filaments. Viruses that replicate in nucleus post-mitotic cells use microtubules dynein-dynactin to traffic nuclear membrane deliver their genome pore complexes (NPCs) into nucleus. How virus particles (virions) or cellular are transferred from NPC is unknown. Here, we analyzed trafficking incoming cytoplasmic adenoviruses by single-particle tracking super-resolution microscopy. We provide evidence for a...
Imaging across scales reveals disease mechanisms in organisms, tissues, and cells. Yet, particular infection phenotypes, such as virus-induced cell lysis, have remained difficult to study. Here, we developed imaging modalities deep learning procedures identify herpesvirus adenovirus (AdV) infected cells without virus-specific stainings. Fluorescence microscopy of vital DNA-dyes live-cell revealed learnable nuclear patterns transferable related viruses the same family. Deep predicted two...
Breast cancer is the most frequently diagnosed in females globally. However, we know relatively little about trends males. This study describes United Kingdom (UK) secular breast from 2000 to 2021 for both sexes. We describe a population-based cohort using UK primary care Clinical Practice Research Datalink (CPRD) GOLD and Aurum databases. There were 5,848,436 eligible 5,539,681 males aged 18+ years, with ≥ one year of prior data availability period. estimated crude incidence rates (IR),...
Large-scale RNAi screening has become an important technology for identifying genes involved in biological processes of interest. However, the quality large-scale is often deteriorated by off-targets effects. In order to find statistically significant effector pathogen entry, we systematically analyzed entry pathways human host cells eight pathogens using image-based kinome-wide siRNA screens with siRNAs from three vendors. We propose a Parallel Mixed Model (PMM) approach that simultaneously...
ABSTRACT Virus infection of humans and livestock can be devastating for individuals populations, sometimes resulting in large economic societal impact. Prevention virus disease by vaccination or antiviral agents is difficult to achieve. A notable exception was the eradication human smallpox over 30 years ago. Today, animals remain susceptible poxvirus infections, including zoonotic transmission. Here we identified a small molecule, bisbenzimide (bisbenzimidazole), its derivatives as potent...
While scientific knowledge of post-COVID-19 condition (PCC) is growing, there remains significant uncertainty in the definition disease, its expected clinical course, and impact on daily functioning. Social media platforms can generate valuable insights into patient-reported health outcomes as content produced at high resolution by patients caregivers, representing experiences that may be unavailable to most clinicians.In this study, we aimed determine validity effectiveness advanced natural...
ABSTRACT We previously identified the bisbenzimide Hoechst 33342 (H42) as a potent multi-stage inhibitor of prototypic poxvirus, vaccinia virus (VACV), and several parapoxviruses. A recent report showed that novel compounds similar in structure to H42 could prevent human cytomegalovirus replication. Here, we assessed whether these also serve poxvirus inhibitors. Using virological assays, show inhibit VACV spread, plaque formation, production infectious progeny with relatively low cell...
Phase imaging is gaining importance due to its applications in fields like biomedical and material characterization. In applications, it can provide quantitative information missing label-free microscopy modalities. One of the most prominent methods phase quantification Transport-of-Intensity Equation (TIE). TIE often requires multiple acquisitions at different defocus distances, which not always feasible a clinical setting hardware constraints. To address this issue, we propose use...
Virological plaque assay is the major method of detecting and quantifying infectious viruses in research diagnostic samples. Furthermore, viral phenotypes contain information about life cycle spreading mechanism virus forming them. While some modernisations have been proposed, conventional typically involves manual quantification phenotypes, which both laborious time-consuming. Here, we present an annotated dataset digital photographs plates Vaccinia - a prototypic propoxvirus. We...
ABSTRACT Cancer cells are susceptible to oncolytic viruses, albeit variably. Human adenoviruses (HAdVs) widely used agents that have been engineered produce progeny within the tumor and elicit bystander effects. We searched for host factors enhancing effects conducted a targeted RNA interference screen against guanine nucleotide exchange (GEFs) of small GTPases. show unfolded protein response (UPR), which is readily inducible in aggressive cells, enhances melanoma or epithelial cancer cell...
Poxvirus egress is a complex process whereby cytoplasmic single membrane–bound virions are wrapped in cell-derived double membrane. These triple-membrane particles, termed intracellular enveloped (IEVs), released from infected cells by fusion. Whereas the wrapping membrane thought to be derived virus-modified trans-Golgi or early endosomal cisternae, cellular factors that regulate virus remain largely undefined. To identify cell required for this prototypic poxvirus, vaccinia (VACV), was...
Adenoviruses (AdVs) are prevalent and give rise to chronic recurrent disease. Human AdV (HAdV) species B C, such as HAdV-C2, -C5, -B14, cause respiratory disease constitute a health threat for immunocompromised individuals. HAdV-Cs well known lysing cells owing the E3 CR1-β-encoded adenovirus death protein (ADP). We previously reported high-throughput image-based screening framework identified an inhibitor of HAdV-C2 multiround infection, nelfinavir mesylate.
This study introduces label-free digital holo-tomographic microscopy (DHTM) and refractive index gradient (RIG) measurements of live, virus-infected cells. We use DHTM to describe virus type-specific cytopathic effects, including cyclic volume changes vaccinia infections, cytoplasmic condensations in herpesvirus rhinovirus distinct from apoptotic work shows for the first time that is suitable observe cells distinguishes signatures under noninvasive conditions. It provides a basis future...
ABSTRACT Adeno-associated virus 2 (AAV2) depends on the simultaneous presence of a helper such as herpes simplex 1 (HSV-1) for productive replication. At same time, AAV2 efficiently blocks replication HSV-1, which would eventually limit its own by diminishing reservoir. This discrepancy begs question how and HSV-1 can coexist in cell population. Here we show that coinfected cultures, DNA takes place almost exclusively S/G -phase cells, while is restricted to G phase. Live microscopy revealed...
ABSTRACT Toxoplasma gondii is an obligate intracellular parasite capable of invading any nucleated cell. Three main clonal lineages (type I, II, III) exist and murine models have driven the understanding general strain-specific immune mechanisms underlying infection. However, are limited for studying parasite-leukocyte interactions in vivo, discrepancies between cellular responses observed mouse versus human cells. Here, we developed a zebrafish infection model to study innate response vivo....
Through digital imaging, microscopy has evolved from primarily being a means for visual observation of life at the micro- and nano-scale, to quantitative tool with ever-increasing resolution throughput. Artificial intelligence, deep neural networks, machine learning are all niche terms describing computational methods that have gained pivotal role in microscopy-based research over past decade. This Roadmap is written collectively by prominent researchers encompasses selected aspects how...