A5010047567- CRISPR and Genetic Engineering
- Zebrafish Biomedical Research Applications
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Animal Genetics and Reproduction
- Mitochondrial Function and Pathology
- Virus-based gene therapy research
- Chromosomal and Genetic Variations
- Pluripotent Stem Cells Research
- Congenital heart defects research
- Metabolism and Genetic Disorders
- Bacterial Genetics and Biotechnology
- RNA regulation and disease
- Stress Responses and Cortisol
- RNA Research and Splicing
- Physiological and biochemical adaptations
- DNA and Nucleic Acid Chemistry
- ATP Synthase and ATPases Research
- RNA modifications and cancer
- Genetics, Aging, and Longevity in Model Organisms
- Genomics and Rare Diseases
- Genetics and Neurodevelopmental Disorders
- Cancer-related gene regulation
- Circadian rhythm and melatonin
Mayo Clinic
2016-2025
Texas A&M University
2024-2025
United Therapeutics (United States)
2025
Mayo Clinic in Arizona
2015-2024
WinnMed
2014-2024
Iowa State University
2018-2023
GlaxoSmithKline (United Kingdom)
2021
Mayo Clinic in Florida
2013-2019
National Center on Birth Defects and Developmental Disabilities
2019
University of Massachusetts Chan Medical School
2018
Abstract Background Recent studies of transcription activator-like (TAL) effector domains fused to nucleases (TALENs) demonstrate enormous potential for genome editing. Effective design TALENs requires a combination selecting appropriate genetic features, finding pairs binding sites based on consensus sequence, and, in some cases, identifying endogenous restriction downstream molecular applications. Results We present the web-based program Mojo Hand designing TAL and TALEN constructs editing...
The bacterial family Enterobacteriaceae is notable for its well studied human pathogens, including Salmonella , Yersinia Shigella and Escherichia spp. However, it also contains several plant pathogens. We report the genome sequence of a pathogenic enterobacterium, Erwinia carotovora subsp. atroseptica ( Eca ) strain SCRI1043, causative agent soft rot blackleg potato diseases. Approximately 33% genes are not shared with sequenced enterobacterial some predicted to facilitate unexpected...
Efficient precision genome engineering requires high frequency and specificity of integration at the genomic target site. Here, we describe a set resources to streamline reporter gene knock-ins in zebrafish demonstrate broader utility method mammalian cells. Our approach uses short homology 24–48 bp drive targeted DNA cassettes by homology-mediated end joining (HMEJ) double strand break gene. vector series, pGTag (plasmids for Gene Tagging), contains reporters flanked universal CRISPR sgRNA...
The Sleeping Beauty (SB) transposon system, derived from teleost fish sequences, is extremely effective at delivering DNA to vertebrate genomes, including those of humans. We have examined several parameters the SB system improve it as a potential, nonviral vector for gene therapy. Our investigation centered on three features: carrying capacity efficient integration into chromosomes HeLa cells, effects overexpression transposase transposition rates, and improvements in activity increase...
Endothelial tubulogenesis is a crucial step in the formation of functional blood vessels during angiogenesis and vasculogenesis. Here, we use vivo imaging living zebrafish embryos expressing fluorescent fusion proteins β-Actin, α-Catenin, ERM family member Moesin1 (Moesin a), to define novel cord hollowing process that occurs initial stages intersegmental (ISVs) embryo. We show primary lumen elongates along cell junctions between at least two endothelial cells embryonic angiogenesis....
Transposons have been used in invertebrates for transgenesis and insertional mutagens genetic screens. We tested a functional transposon called Sleeping Beauty the one-cell mouse embryo. In this report, we describe experiments which vectors were injected into embryos with mRNA expressing SB10 transposase enzyme. Molecular evidence of transposition was obtained by cloning insertion sites from multiple transgenic mice produced mRNA/transposon coinjection. also demonstrate germ-line...
Tobacco use is predicted to result in over 1 billion deaths worldwide by the end of 21 st century. How genetic variation contributes observed differential predisposition human population drug dependence unknown. The zebrafish ( Danio rerio ) an emerging vertebrate model system for understanding genetics behavior. We developed a nicotine behavioral assay and applied it forward screen using gene-breaking transposon mutagenesis. used this method molecularly characterize bdav /cct8 hbog...
One key problem in precision genome editing is the unpredictable plurality of sequence outcomes at site targeted DNA double stranded breaks (DSBs). This due to typical activation versatile Non-homologous End Joining (NHEJ) pathway. Such unpredictability limits utility somatic gene for applications including therapy and functional genomics. For germline work, accurate reproduction identical alleles using NHEJ a labor intensive process. In this study, we propose Microhomology-mediated (MMEJ)...
Abstract We have identified the zebrafish homologue of VE‐cadherin and documented its expression in developing vascular system. The gene is specifically expressed endothelial cell lineage beginning with differentiation migration angioblasts persists throughout vasculogenesis, angiogenesis, endocardium development. Staining embryos by whole‐mount situ hybridization probe provides a method to screen for defects. To illustrate this utility, we used demonstrate conservation growth factor‐A...
Genome editing appears poised to enter an exciting new era. Targeted double-stranded breaks due custom restriction enzymes are powerful nucleating events for the induction of local changes in genome. The zinc finger nuclease (ZFN) platform established potential this approach zebrafish, but access high quality reagents has been a major bottleneck field. However, two groups recently report successful somatic and germline gene modification using architecture, transcription activator-like...