A5060468053- Calcium Carbonate Crystallization and Inhibition
- Cellular Mechanics and Interactions
- Virus-based gene therapy research
- Lipid Membrane Structure and Behavior
- Corneal surgery and disorders
- Bone Tissue Engineering Materials
- Paleontology and Stratigraphy of Fossils
- Robot Manipulation and Learning
- Motor Control and Adaptation
- Muscle activation and electromyography studies
- Proteoglycans and glycosaminoglycans research
- Cephalopods and Marine Biology
- Animal Virus Infections Studies
- Cardiomyopathy and Myosin Studies
- Ophthalmology and Visual Impairment Studies
- Advanced Fluorescence Microscopy Techniques
- Ocular Surface and Contact Lens
- Force Microscopy Techniques and Applications
- AI in cancer detection
- Glaucoma and retinal disorders
- Dermatologic Treatments and Research
- Liver Disease Diagnosis and Treatment
- Vitamin K Research Studies
- Metabolomics and Mass Spectrometry Studies
- COVID-19 diagnosis using AI
Boehringer Ingelheim (Germany)
2018-2024
Max Planck Institute of Biochemistry
2012-2024
Technische Universität Berlin
2015-2016
Klinikum Ernst von Bergmann
2009-2015
Ophthalmology Clinic
2009-2015
Roche Pharma AG (Germany)
2015
Roche (Switzerland)
2014
Max Planck Society
2012-2013
University of Bremen
2006-2012
Technische Universität Dresden
2011-2012
Cell cortex remodeling during cell division is a result of myofilament-driven contractility the cortical membrane-bound actin meshwork. Little known about interaction between individual myofilaments and filaments. Here we reconstituted minimal to directly visualize action on filaments using TIRF microscopy. We show that synthetic fragment compact while processively moving along propose mechanism by which tension builds up ends myofilaments, resulting in compressive stress exerted single...
Abstract Non-alcoholic fatty liver disease (NAFLD) and the progressive form of non-alcoholic steatohepatitis (NASH) are diseases major importance with a high unmet medical need. Efficacy studies on novel compounds to treat NAFLD/NASH using models frequently evaluated established histological feature scores ballooning, inflammation, steatosis fibrosis. These features assessed by trained pathologist microscopy assigned discrete scores. We demonstrate how automate these convolutional neural...
Smoking is a leading risk factor of chronic obstructive pulmonary disease (COPD), that characterized by lung inflammation, tissue remodeling and emphysema. Although inflammation critical to COPD pathogenesis, the cellular molecular basis underlying smoking-induced pathology remains unclear. Using murine smoke models single-cell RNA-sequencing, we show smoking establishes self-amplifying inflammatory loop an influx molecularly heterogeneous neutrophil subsets excessive recruitment...
Abstract Cigarette smoke (CS) is the leading risk factor to develop COPD. Therefore, pathologic effects of whole CS on differentiation primary small airway epithelial cells (SAEC) were investigated, using from three healthy donors and COPD patients, cultured under ALI (air-liquid interface) conditions. The analysis physiology demonstrated that impaired barrier formation reduced cilia beat activity. Although, COPD-derived cultures preserved some features known CS-induced similarly pronounced...
Non-alcoholic fatty liver disease (NAFLD) affects about 24% of the world's population. Progression early stages NAFLD can lead to more advanced form non-alcoholic steatohepatitis (NASH), and ultimately cirrhosis or cancer. The current gold standard for diagnosis assessment NAFLD/NASH is biopsy followed by microscopic analysis a pathologist. Kleiner score frequently used semi-quantitative progression. In this scoring system features active injury (steatosis, inflammation, ballooning)...
Fluorescence correlation spectroscopy (FCS) measurements are widely used for determination of diffusion coefficients lipids and proteins in biological membranes. In recent years, several variants FCS have been introduced. However, a comprehensive comparison these methods on identical systems has so far lacking. addition, there exist no consistent values already determined well-known or membrane systems. This study aims to contribute better comparability experiments membranes by determining...
Snap, crackle and pop! A simple method to obtain solvent-free, free-standing membranes suspended over apertures with 2.5 μm in diameter is described. The are obtained by bursting of giant unilamellar vesicles (GUV) on a holey surface (see picture). Membrane integrity after fostered enhancing the electrostatic interaction lipids.
Oncolytic virotherapy is thought to result in direct virus-induced lytic tumour killing and simultaneous activation of innate tumour-specific adaptive immune responses. Using a chimeric vesicular stomatitis virus variant VSV-GP, we addressed the oncolytic effects role anti-tumour induction syngeneic mouse lung cancer model LLC1.To study system with limited antiviral effects, generated interferon receptor-deficient cells (LLC1-IFNAR1-/-). Therapeutic efficacy VSV-GP was assessed vivo C57BL/6...
The actin cell cortex in eukaryotic cells is a key player controlling and maintaining the shape of cells, driving major changes such as cytokinesis. It thereby constantly being remodeled. Cell require forces acting on membranes that are generated by interplay membrane coupled filaments assemblies myosin motors. Little known about how their interaction regulates remodeling changes. Because vital importance actin, motors membrane, selective vivo experiments manipulations often difficult to...
Mutations in the human lamin A gene (LMNA) cause a wide range of diseases (laminopathies). Among these is Hutchinson-Gilford progeria syndrome (HGPS), rare premature aging disease. Most HGPS patients carry silent point mutation, which activates cryptic splice site resulting expression permanently isoprenylated and truncated AΔ50/progerin. Another type mutant namely, E145K-lamin A, also causes HGPS. induces profound changes nuclear architecture patient cells as well after cultured cells. The...
Observations of human grasping reveal that the exploitation environmental constraints is a key structural aspect for robustness and versatility behavior. We analyze 3,400 trials with 17 subjects 25 objects to show viewing as central yields surprisingly simple representations present hypothesis-driven experiments emphasize centrality in extract from data “grasping plan” generative model all we observed. This plan can principle be transferred robot system an attempt leverage improve...
Preclinical studies of novel compounds rely on quantitative readouts from animal models. Frequently employed histopathological tissue scoring are time consuming, require highly specialized staff and subject to inherent variability. Recent advances in deep convolutional neural networks (CNN) now allow automating such tasks. Here, we demonstrate this for the case Ashcroft fibrosis score a newly developed inflammation characterize fibrotic inflammatory lung diseases. Sections mice exhibiting...
During the growth process of nacre layer in abalone shells, aragonite platelets are built into a preformed organic matrix. This matrix consists chitin and different proteins that may affect morphology crystal structure developed platelets. The can be divided two fractions: soluble insoluble has influence on precipitation rates calcium carbonate crystals also affects growth. In this work we investigated collective crystallization device which contained was flowed through by CaCl2 NaHCO3...
As a first step towards transferring human grasping capabilities to robots, we analyzed the behavior of subjects. We derived taxonomy in order adequately represent observed strategies. During analysis recorded data, this classification scheme helped us obtain better understanding behavior. will provide support for our hypothesis that humans exploit compliant contact between hand and environment compensate uncertainty. also show realization resulting strategies on real robot. It is belief...
Recently, a new and versatile assay to determine the partitioning coefficient [Formula: see text] as measure for affinity of peripheral membrane proteins lipid bilayers was presented in research article entitled, "Introducing fluorescence-based standard quantify protein into membranes" [1]. Here, well-characterized binding hexahistidine-tag (His6) NTA(Ni) utilized. Complementarily, this data reports average diffusion His6-tagged enhanced green fluorescent (eGFP-His6) analog ATTO-647N-DOPE...