A5008858921- Nicotinic Acetylcholine Receptors Study
- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Lipid Membrane Structure and Behavior
- Insect and Pesticide Research
- Venomous Animal Envenomation and Studies
- Toxin Mechanisms and Immunotoxins
- Neurobiology and Insect Physiology Research
- Cholinesterase and Neurodegenerative Diseases
- Biochemical and Structural Characterization
- Antimicrobial Peptides and Activities
- RNA and protein synthesis mechanisms
- Ion Transport and Channel Regulation
- Ion Channels and Receptors
- Neuroinflammation and Neurodegeneration Mechanisms
- Cardiac electrophysiology and arrhythmias
- Monoclonal and Polyclonal Antibodies Research
- Protein Structure and Dynamics
- Biochemical effects in animals
- Glycosylation and Glycoproteins Research
- Angiogenesis and VEGF in Cancer
- ATP Synthase and ATPases Research
- Alzheimer's disease research and treatments
- 14-3-3 protein interactions
Shenzhen University
2022-2025
Institute of Bioorganic Chemistry
2016-2025
Lomonosov Moscow State University
2016-2025
Shenzhen MSU-BIT University
2023-2025
Moscow Power Engineering Institute
2019-2024
Moscow Aviation Institute
2019-2024
Moscow Institute of Physics and Technology
2018-2024
Shenzhen Technology University
2024
Moscow State University
2014-2024
Living Systems (United States)
2023
How aging affects cells of the human brain active milieu remains largely unknown. Here, we analyze astrocytes and neurons in neocortical tissue younger (22-50 years) older (51-72 adults. Aging decreases amount reduced mitochondrial cytochromes but not neurons. The protein-to-lipid ratio increases Aged show morphological atrophy quantified by decreased length branches, volume fraction leaflets, shrinkage anatomical domain. Atrophy correlates with loss gap junction coupling between increased...
Discovery of proteins expressed in the central nervous system sharing three-finger structure with snake α-neurotoxins provoked much interest to their role brain functions. Prototoxin LYNX1, having homology both Ly6 and neurotoxins, is first identified member this family membrane-tethered by a GPI anchor, which considerably complicates vitro studies. We report for time NMR spatial water-soluble domain human LYNX1 lacking anchor (ws-LYNX1) its concentration-dependent activity on nicotinic...
SLURP-1 is a secreted toxin-like Ly-6/uPAR protein found in epithelium, sensory neurons and immune cells. Point mutations the slurp-1 gene cause autosomal inflammation skin disease Mal de Meleda. considered an autocrine/paracrine hormone that regulates growth differentiation of keratinocytes controls malignant cell transformation. The majority previous studies have been made using fusion constructs containing, addition to native protein, extra polypeptide sequences. Here we describe activity...
Abstract Calorie-restricted (CR) diet has multiple beneficial effects on brain function. Here we report morphological and functional changes in hippocampal astrocytes 3-months-old mice subjected to 1 month of the diet. Whole-cell patch-clamp recordings were performed CA1 stratum (str.) radiatum slices. The cells also loaded with fluorescent dye through patch pipette. CR did not affect number astrocytic branches but increased volume fraction (VF) distal perisynaptic leaflets. astrocyte growth...
Abstract Aim A high‐fat diet (HFD) is generally considered to negatively influence the body, brain, and cognition. Nonetheless, fat fatty acids are essential for nourishing constructing brain tissue. Astrocytes central lipolysis metabolism. We tested how HFD affects astrocyte metabolism, morphology, physiology. Methods used Raman microspectroscopy assess redox state of mitochondria lipid content in astrocytes neurons hippocampal slices mice subjected HFD. were loaded with fluorescent dye...
Inflammation is a physiological response of the immune system to infectious agents or tissue injury, which involves cascade vascular and cellular events activation biochemical pathways depending on type harmful agent stimulus generated. The Kunitz peptide HCIQ2c1 sea anemone Heteractis magnifica strong protease inhibitor exhibits neuroprotective analgesic activities. In this study, we investigated anti-inflammatory potential in histamine- lipopolysaccharide (LPS)-activated RAW 264.7...
The choice of a suitable detergent-based membrane mimetic is crucial importance for high-resolution NMR studies proteins. present report describes new approach detergent screening. It based on the comparison 2D 1H,15N-correlation spectra protein in membrane-bilayer "reference" medium and "trial" environments. proposed lipid−protein nanodisc (LPN) representing nanoscale phospholipid bilayers wrapped around by apolipoprotein A-1. set zwitterionic (DPC, DMPC/DHPC), anionic (SDS, LMPG, LPPG),...
The predicted Exigobacterium sibiricum bacterirhodopsin gene was amplified from an ancient Siberian permafrost sample. protein bacteriorhodopsin Exiguobacterium (ESR) encoded by this expressed in Escherichia coli membrane. ESR bound all‐trans‐retinal and displayed absorbance maximum at 534 nm without dark adaptation. photocycle is characterized fast formation of M intermediate the presence a significant amount O intermediate. Proteoliposomes with incorporated transport protons outward...
The structure and dynamics of the isolated voltage-sensing domain (VSD) archaeal potassium channel KvAP was studied by high-resolution NMR. almost complete backbone resonance assignment partial side-chain 2H,13C,15N-labeled VSD were obtained for protein solubilized in DPC/LDAO (2:1) mixed micelles. Secondary tertiary structures characterized using secondary chemical shifts NOE contacts. These data indicate that spatial micelles corresponds to an open state channel. contacts amide protons...
Human-secreted Ly-6/uPAR-related protein-2 (SLURP-2) regulates the growth and differentiation of epithelial cells. Previously, auto/paracrine activity SLURP-2 was considered to be mediated via its interaction with α3β2 subtype nicotinic acetylcholine receptors (nAChRs). Here, we describe structure pharmacology a recombinant analogue SLURP-2. Nuclear magnetic resonance spectroscopy revealed 'three-finger' fold conserved β-structural core three protruding loops. Affinity purification using...
In the present Communication we demonstrate possibility to use high-resolution NMR for investigation of membrane proteins in reconstituted high-density lipoprotein (rHDL) particles. The rHDL particles are nanoscale phospholipid bilayers wrapped around by a dimer apolipoprotein A-1 (Bayburt, T. H.; Grinkova, Y. V.; Sligar, S. G. Nano Lett. 2002, 2, 853−856). contrast commonly used spherical micelles, incorporate lipid bilayer like biological membranes. These still undergo isotropic motion on...
Weak toxin from Naja kaouthia (WTX) belongs to the group of nonconventional "three-finger" snake neurotoxins. It irreversibly inhibits nicotinic acetylcholine receptors and allosterically interacts with muscarinic (mAChRs). Using site-directed mutagenesis, NMR spectroscopy, computer modeling, we investigated recombinant mutant WTX analogue (rWTX) which, compared native toxin, has an additional N-terminal methionine residue. In comparison wild-type rWTX demonstrated altered pharmacological...
Nicotinic acetylcholine receptors (nAChRs) are a promising target for development of new anticancer therapies. Here we have investigated the effects endogenous human proteins SLURP-1 and SLURP-2, antagonists nAChRs, on epithelial cancer cells.Growth cells (A431, SKBR3, MCF-7, A549, HT-29) exposed to SLURP-1, mecamylamine, atropine, timolol gefitinib was measured by WST-1 test. Expression levels α7-nAChR EGF their distribution in were studied confocal microscopy flow cytometry. Secretion A431...
Lynx1 regulates synaptic plasticity in the brain by regulating nicotinic acetylcholine receptors (nAChRs). It is not known to which extent can bind endogenous nAChR subunits or how this interaction affected Alzheimer's disease pathology. We apply affinity purification demonstrate that a water-soluble variant of human (Ws-Lynx1) isolates α3, α4, α5, α6, α7, β2, and β4 from rat cortical extracts, midbrain olfactory bulb suggesting forms complexes with multiple subtypes rodent brain. Incubation...