A5076951365- Molecular Biology Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Polymer Surface Interaction Studies
- Microfluidic and Capillary Electrophoresis Applications
- Bone Tissue Engineering Materials
- Innovative Microfluidic and Catalytic Techniques Innovation
- Biosensors and Analytical Detection
- RNA and protein synthesis mechanisms
- Biotin and Related Studies
- Molecular Junctions and Nanostructures
- Nanofabrication and Lithography Techniques
- Acute Lymphoblastic Leukemia research
- Orthopaedic implants and arthroplasty
- Blood properties and coagulation
- Genetic factors in colorectal cancer
- SARS-CoV-2 detection and testing
- Hair Growth and Disorders
- Caveolin-1 and cellular processes
- Force Microscopy Techniques and Applications
- Arts, Culture, and Music Studies
- Total Knee Arthroplasty Outcomes
- Museums and Cultural Heritage
- German Literature and Culture Studies
- Cellular transport and secretion
Hahn-Schickard-Gesellschaft für angewandte Forschung
2019-2025
University of Freiburg
2014-2021
Johannes Gutenberg University Mainz
2011-2013
European Molecular Biology Laboratory
1992
Whitehead Institute for Biomedical Research
1988
In simple epithelial cells, apical and basolateral proteins are sorted into separate vesicular carriers before delivery to the appropriate plasma membrane domains. To dissect putative sorting machinery, we have solubilized Golgi-derived transport vesicles with detergent CHAPS shown that an marker, influenza haemagglutinin (HA), formed a large complex together several integral proteins. Remarkably, similar set of CHAPS-insoluble was found after solubilization total cellular fraction. This...
Murine erythroleukemia (MEL) cells represent a valuable system to study the biogenesis of cytoskeleton during erythroid differentiation. When attached fibronectin-coated dishes MEL induce, upon addition DMSO, 7-d differentiation process which they enucleate and reach reticulocyte stage (Patel, V. P., H. F. Lodish. 1987. J. Cell Biol. 105:3105-3118); accumulate band 3, spectrin, ankyrin in amounts equivalent those found mature red blood cells. To follow biosynthesis spectrin differentiation,...
It is well-known that protein-modified implant surfaces such as TiO2 show a higher bioconductivity. Fibronectin glycoprotein from the extracellular matrix (ECM) with major role in cell adhesion. can be applied on titanium oxide to accelerate integration. Not only surface concentration but also presentation of protein plays an important for cellular response. We were able TiOX modified biotinylated fibronectin adsorbed streptavidin-silane self-assembly multilayer system are more effective...
Colorectal cancer (CRC) remains a major global health challenge, with an increasing incidence of early-onset cases among young adults. Targeted analysis cell-free DNA (cfDNA) methylation in blood has emerged as promising minimally invasive diagnostic approach. While digital PCR (dPCR) offers high sensitivity and low turnaround times, conventional bisulfite-based dPCR assays require large plasma volumes due to cfDNA degradation, limiting clinical feasibility. To overcome this limitation, we...
Primer and probe sequence designs are among the most critical input factors in real-time polymerase chain reaction (PCR) assay optimization. In this study, we present use of statistical design experiments (DOE) approach as a general guideline for optimization more specifically focus on label-free hydrolysis probes that designated mediator (MPs), which used reverse transcription MP PCR (RT-MP PCR). The effect three performance was investigated: distance between primer cleavage site; dimer...
Biofunctionalized implant surfaces may accelerate bony integration and increase long-term stability.The aim of the study was to evaluate osseous reaction toward biomimetic titanium implants coated with quasicovalent immobilized fibronectin in an vivo animal model.A total 84 (uncoated [control 1, n = 36], streptavidin-biotin [test 24], streptavidin-biotin-fibronectin 2, 24]) were inserted 1 mm supracortically proximal tibia 12 rabbits. The samples examined after 3 6 weeks. Total bone-implant...
Time-independent, serial valving under rotation on centrifugal microfluidic platforms by a fill-level-coupled temperature change rate (FLC-TCR) actuated principle.
In this study we optimised the fluorescence signal generation of contact quenched universal reporter oligonucleotides.
Abstract Non‐fouling TiO 2 surfaces are attractive for a wide range of applications such as biosensors and medical devices, where biologically inert needed. Typically, this is achieved by controlled surface modifications which prevent protein adsorption. For example, polyethylene glycol (PEG) or PEG‐derived polymers have been widely applied to render inert. These further modified in order achieve specific bio‐activation. Therefore, there efforts specifically functionalize with embedded...
Mediator probe (MP) PCR is a real-time approach that uses standardized universal fluorogenic reporter oligonucleotides (UR) in conjunction with label-free sequence-specific probes. To enable multiplex MP PCR, we designed set of five optimized URs different fluorescent labels. Performance the was verified for detection pentaplex food panel and quadruplex methicillin-resistant Staphylococcus aureus (MRSA) panel. Results were comparable to corresponding hydrolysis (HP) also designated as TaqMan...
Digital PCR (dPCR) is a powerful method for highly sensitive and precise quantification of nucleic acids. However, designing optimizing new multiplex dPCR assays using target sequence specific probes remains cumbersome, since fluorescent signals must be optimized every panel. As solution, we established generic fluorogenic 6-plex reporter set, based on mediator probe technology, that decouples detection from signal generation. This set compatible with different panels thus provides already...
The biomodification of surfaces, especially titanium, is an important issue in current biomedical research. Regarding it also to ensure a specific protein modification its surface because here binding that too random can be observed. Specific nanoscale architectures applied overcome this problem. As recently shown, streptavidin used as coupling agent immobilize biotinylated fibronectin (bFn) on TiO(X) surface. Because the conformation adsorbed monolayer, possible adsorb more and layers. On...
Increasing cell adhesion on implant surfaces is an issue of high biomedical importance. Early colonization with endogenous cells reduces the risk bacterial contamination and enhances integration into diverse cellular tissues surrounding it. In vivo implants controlled by a complex spatial temporal interplay cytokines adhesive molecules. The concept multi-biofunctionalized TiO2 surface for stimulating bone soft tissue growth presented here. All supramolecular architectures were built...
There is an increasing demand for optimization-free multiplex assays to rapidly establish comprehensive target panels cancer monitoring by liquid biopsy. We present the mediator probe (MP) PCR quantification of seven most frequent point mutations and corresponding wild types (KRAS BRAF) in colorectal carcinoma. Standardized parameters digital assay were derived using design experiments. Without further optimization, limit detection (LoD) was determined through spiking experiments with...
We demonstrate detection and quantification of bacterial load with a novel microfluidic one-pot wash-free fluorescence in situ hybridization (FISH) assay droplets. The method offers minimal manual workload by only requiring mixing the sample reagents loading it into cartridge. By centrifugal step emulsification, our partitioned 210 pL (73 µm diameter) droplets for encapsulation followed permeabilization, hybridization, signal detection. Employing locked nucleic acid (LNA)/DNA molecular...
RNA interference (RNAi) is a powerful therapeutic approach for messenger (mRNA) level regulation in human cells. RNAi can be triggered by small interfering RNAs (siRNAs) which are delivered non-viral carriers, e.g., dendriplexes. siRNA quantification inside carriers essential drug delivery system development. However, current measuring methods either not very sensitive, only semi-quantitative or specific towards intact target sequences. We present novel reverse transcription real-time PCR...
Concept of reporter emission multiplexing (REM) to increase capacities in digital PCR by using population specific reporters (PSR) labeled with fluorophores different signal intensities.
A centrifugal microfluidic system for automated, highly sensitive and multiplexed qPCR analyses, demonstrated minimal residual disease monitoring in acute lymphoblastic leukaemia.