A5051784859- Hemodynamic Monitoring and Therapy
- Cardiac, Anesthesia and Surgical Outcomes
- Sepsis Diagnosis and Treatment
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Education, Psychology, and Social Research
- Cardiac Arrest and Resuscitation
- Non-Invasive Vital Sign Monitoring
- Liver physiology and pathology
- Respiratory Support and Mechanisms
- Renal function and acid-base balance
- COVID-19 Clinical Research Studies
- Inorganic and Organometallic Chemistry
- Intensive Care Unit Cognitive Disorders
- Fungal Biology and Applications
- Chemical Synthesis and Reactions
- Organ Transplantation Techniques and Outcomes
- Anesthesia and Sedative Agents
- Plant and fungal interactions
- Receptor Mechanisms and Signaling
- Trauma and Emergency Care Studies
- Chemical Synthesis and Analysis
- Neuroscience and Neuropharmacology Research
- Acute Kidney Injury Research
- Effects of Radiation Exposure
- Traumatic Brain Injury and Neurovascular Disturbances
Charles University
2016-2025
University Hospital Plzen
2008-2023
Institute of Clinical and Experimental Medicine
2021-2023
University Hospital Hradec Králové
2021
Jan Evangelista Purkyně University in Ústí nad Labem
2021
Karlovac University of Applied Sciences
2020
Biomedical Research Center of the Slovak Academy of Sciences
2019-2020
Opća bolnica Karlovac
2020
Resuscitation Council
2012-2019
Edwards Lifesciences (Switzerland)
2016
Abstract Introduction Stroke volume variation (SVV) is a good and easily obtainable predictor of fluid responsiveness, which can be used to guide therapy in mechanically ventilated patients. During major abdominal surgery, inappropriate management may result occult organ hypoperfusion or overload patients with compromised cardiovascular reserves thus increase postoperative morbidity. The aim our study was evaluate the influence SVV guided optimization on functions morbidity high risk...
Meropenem bactericidal activity depends on the time when free drug concentrations remain above minimum inhibitory concentration of pathogens. The goal this study was to compare clinical and bacteriological efficacy continuous meropenem infusion versus bolus administration in critically ill patients with severe infection, evaluate safety both dosing regimens.Patients admitted interdisciplinary Intensive Care Unit (ICU) who suffered from infections received were randomized either Infusion...
In almost half of all sepsis patients, acute kidney injury (AKI) develops. However, the pathobiologic differences between patients with and without AKI are only poorly understood. We used a unique opportunity to examine dynamic inflammatory, renal hemodynamic, microvascular changes in two clinically relevant large-animal models sepsis. Our aim was assess variability responses identify both hemodynamic nonhemodynamic mechanisms discriminating individuals from those whom did not develop....
Hypercapnic acidosis (HCA) that accompanies lung-protective ventilation may be considered permissive (a tolerable side effect), or it therapeutic by itself. Cardiovascular effects contribute to, limit, the potential impact of HCA; therefore, a complex physiological study was performed in healthy pigs to evaluate systemic and organ-specific circulatory HCA, compare them with those metabolic (eucapnic) (MAC).In anesthetized, mechanically ventilated instrumented pigs, HCA induced increasing...
Objectives: To investigate the potential benefits of vagus nerve stimulation in a clinically-relevant large animal model progressive sepsis. Design: Prospective, controlled, randomized trial. Setting: University research laboratory. Subjects: Twenty-five domestic pigs were divided into three groups: 1) sepsis group (eight pigs), 2) + (nine and 3) control sham pigs). Interventions: Sepsis was induced by cultivated autologous feces inoculation anesthetized, mechanically ventilated, surgically...
Abstract Mechanisms of right ventricular (RV) dysfunction in heart failure (HF) are poorly understood. RV response to volume overload (VO), a common contributing factor HF, is rarely studied. The goal was identify interventricular differences chronic VO. Rats underwent aorto-caval fistula (ACF)/sham operation induce After 24 weeks, and left (LV) functions, gene expression proteomics were ACF led biventricular dilatation, systolic hypertrophy affecting relatively more RV. Increased afterload...
To hypothesize that reduced L-type calcium current with consequent shortening of cardiac repolarization is present in a clinically relevant porcine model hyperdynamic septic shock. Myocardial depression well-recognized manifestation sepsis and Reduction was demonstrated to contribute the myocardial endotoxemic rodents.Laboratory animal experiments.Animal research laboratory at university.Twenty-two domestic pigs either gender.In anesthetized, mechanically ventilated, instrumented pigs,...
The use of goal directed fluid protocols in intermediate risk patients undergoing hip or knee replacement was studied few trials using invasive monitoring. For this reason we have implemented two different management protocols, both based on a novel totally non-invasive arterial pressure monitoring device and compared them to the standard (no-protocol) treatment applied before transition our academic institution. Three groups were prospective study: observational (CONTROL, N = 40) group...
Background and objectives: Treatment with mesenchymal stem cells (MSCs) has elicited considerable interest as an adjunctive therapy in sepsis. However, the encouraging effects of experiments MSC rodents have not been adequately studied large-animal models better relevance to human Here, we aimed assess safety efficacy bone marrow-derived MSCs a clinically relevant porcine model progressive peritonitis-induced Methods: Thirty-two anesthetized, mechanically ventilated, instrumented pigs were...
(i) Purpose. The fluid challenge (FC) is a well-established test of preload reserve. Only limited data exist in regard to the FC efficacy based on infusion time. Slow administration may be associated with lack effect redistribution and external conditions changes. On contrary, fast lead brisk overload damage endothelium endothelial glycocalyx (EG). aim this trial was compare time its hemodynamic effects EG. (ii) Methods. Prospective randomized single-center (5-10 minutes) versus slow (20-30...
Abstract Background The pathophysiology of sepsis-induced acute kidney injury remains elusive. Although mitochondrial dysfunction is often perceived as the main culprit, data from preclinical models yielded conflicting results so far. aim this study was to assess immune-metabolic background sepsis-associated renal using sequential biopsy approach with mitochondria function evaluation in a large clinically relevant porcine mimicking two different paces and severity sepsis couple traditional...
A homologous series of novel nitro-catechol structures (7a-7e) were synthesized and tested as inhibitors the enzyme catechol-O-methyltransferase (COMT). Increasing chain length was found to have significant impact on both brain penetration duration COMT inhibition in rat. Of this series, compound 7b (1-(3,4-dihydroxy-5-nitrophenyl)-2-phenyl-ethanone) exhibit most potent selective peripheral COMT, with an profile more similar entacapone 2 than tolcapone 1 (an equipotent central inhibitor) but...
Perioperative goal directed therapy (GDT) can substantially improve the outcomes of high risk surgical patients as shown by many clinical studies. However, approach needs initial investment and increase already very staff workload. These economic imperatives may be at least partly responsible for weak adherence to GDT concept. A few models are available evaluation cost-effectiveness, but studies real data based on a recent trial lacking. In order address this we have performed retrospective...
Accumulating evidence suggests that heart rate variability (HRV) alterations could serve as an indicator of sepsis progression and outcome, however, the relationships HRV major pathophysiological processes remain unclear. Therefore, in this experimental study was investigated a clinically relevant long-term porcine model severe sepsis/septic shock. analyzed by several methods parameters were correlated with sepsis. In 16 anesthetized, mechanically ventilated instrumented domestic pigs either...
The complex pathogenesis of sepsis and septic shock involves myocardial depression, the pathophysiology which, however, remains unclear. In this study, cellular mechanisms depression were addressed in a clinically relevant, large animal (porcine) model shock. Sepsis was induced by fecal peritonitis 8 anesthetized, mechanically ventilated, instrumented pigs both sexes continued for 24 hours. control pigs, an identical experiment but without induction performed. vitro analysis cardiac function...