A5079810953- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Traumatic Brain Injury and Neurovascular Disturbances
- Cardiac Arrest and Resuscitation
- Sepsis Diagnosis and Treatment
- Hemodynamic Monitoring and Therapy
- Blood transfusion and management
- Anesthesia and Neurotoxicity Research
- Renal function and acid-base balance
- Electrolyte and hormonal disorders
- Cardiac, Anesthesia and Surgical Outcomes
- Acute Kidney Injury Research
- Anesthesia and Sedative Agents
- Acute Ischemic Stroke Management
- Occupational Health and Performance
- Cancer, Stress, Anesthesia, and Immune Response
- Anesthesia and Pain Management
- Hydrogen's biological and therapeutic effects
- Thermal Regulation in Medicine
- Cardiac electrophysiology and arrhythmias
- Restraint-Related Deaths
- Multiple Sclerosis Research Studies
- Alcoholism and Thiamine Deficiency
- Sleep and Work-Related Fatigue
- Community Development and Social Impact
- Muscle and Compartmental Disorders
University Hospital Hradec Králové
2015-2025
University of Hradec Králové
2018-2025
Charles University
2014-2025
Technical University of Liberec
2023-2025
(i) Purpose. The fluid challenge (FC) is a well-established test of preload reserve. Only limited data exist in regard to the FC efficacy based on infusion time. Slow administration may be associated with lack effect redistribution and external conditions changes. On contrary, fast lead brisk overload damage endothelium endothelial glycocalyx (EG). aim this trial was compare time its hemodynamic effects EG. (ii) Methods. Prospective randomized single-center (5-10 minutes) versus slow (20-30...
Background The ischemia-reperfusion injury (IRI) is unavoidable in vascular surgery. Damage to the microcirculation and endothelial glycocalyx might set up a shock with loss of circulatory coherence organ failure. Sulodexide may help protect alleviate injury. Methods Twenty female piglets underwent surgery 30-min-long suprarenal aortic clamp, followed by two hours reperfusion. Ten received sulodexide before 10 normal saline. Blood urine samples were taken at baseline 20-min intervals until...
Background: Sulodexide is a glycosaminoglycan-based drug prescribed to patients with angiopathy. We performed pilot study investigate whether sulodexide positively modulates the endothelial glycocalyx (EG) layer and microcirculation in porcine model of EG enzymatic damage. The sugar-based lining that involved physiology capillary wall pathogenesis many diseases. Methods: damage was induced eight piglets by hyaluronidase III heparanase I given intravenously. Four animals received 600 IU...
It was aimed to determine whether there is a statistically significant difference between various types of displaced supracondylar fractures the humerus in children with movement impairment according Flynn's classification. Clinical results 263 patients who were operated on closed reduction and percutaneous pinning for fracture evaluated. classification used compare impairment. One year after procedure, only one patient category extension type II displacement, elbow flexion, had an...
Coronavirus disease (COVID-19) associated endotheliopathy and microvascular dysfunction are of concern.The objective the present single-center observational pilot study was to compare endothelial glycocalyx (EG) damage in patients with severe COVID-19 (COVID-19 group) bacterial pneumonia septic shock (non-COVID group).Biomarkers EG (syndecan-1), cells (EC) (thrombomodulin), activation (P-selectin) were measured blood on three consecutive days from admission intensive care unit (ICU). The...
The pathogenesis of multiple sclerosis (MS) remains elusive. Recent reports advocate greater involvement B cells and immunoglobulins in the initiation propagation MS lesions at different stages their ontogeny. key role was initially identified by studies which patients whose fulminant attacks demyelination did not respond to steroids experienced remarkable functional improvement following plasma exchange. positive response Rituximab Phase II clinical trials relapsing-remitting confirms...
The endothelial glycocalyx (EG) lining the endoluminal surface of capillaries has been proposed as a key component microcirculation and major player in microvascular pathology. Recent advances understanding its physiological role clinical significance have made upon development methods allowing EG assessment medicine. Laboratory can assess amount damage by measuring levels degradation products (e.g. syndecan-1, heparan sulphate hyaluronan sulphate), mostly plasma, however, their turnover...
Hydrogen is a potent antioxidant agent that can easily be administered by inhalation. The aim of the study was to evaluate whether hydrogen protects endothelial glycocalyx layer after successful cardiopulmonary resuscitation (CPR).Fourteen anesthetized pigs underwent CPR induced ventricular fibrillation. During and return spontaneous circulation, 2% gas seven (hydrogen group) constituted control group. Biochemistry sublingual microcirculation were assessed at baseline, during CPR, 15th,...
Meropenem dosing for septic critically patients is difficult due to pathophysiological changes associated with sepsis as well supportive symptomatic therapies. A prospective single-center study assessed whether fluid retention alters meropenem pharmacokinetics and the achievement of pharmacokinetic/pharmacodynamic (PK/PD) targets efficacy.Twenty-five ICU (19 m, 6f) aged 32-86 years mean APACHE II score 20.2 (range 11-33), suffering mainly from perioperative intra-abdominal or respiratory...
Adhesions are the most common cause of long-term morbidity after abdominal surgery and often various forms intestinal passage disorders ranging from partial obstruction to complete, life-threatening obstruction. The aim present study was evaluate protective effect intraperitoneally administered lipid emulsions on formation adhesions in larger animal model, as lubricating phospholipids mechanical barrier component combined with anti-inflammatory fish oil.Thirty-one female domestic pigs were...
Hyperosmolar solutions have been used in neurosurgery to modify brain bulk and prevent neurological deterioration. The aim of this animal study was compare the short-term effects equivolemic, equiosmolar mannitol hypertonic saline (HTS) on cerebral cortical microcirculation a rabbit craniotomy model.Rabbits (weight, 2.0-3.0 kg) were anesthetized, ventilated mechanically, subjected craniotomy. animals allocated randomly receive 3.75 ml/kg intravenous infusion either 3.2% HTS (group HTS, n =...
Damage of the endothelial glycocalyx (EG) has been described during surgery, but effect different anesthesia techniques remains unknown. Perfused boundary region (PBR) evaluated by side-stream dark field (SDF) imaging sublingual microcirculation enables in vivo EG assessment. PBR values are inversely related to thickness.The aim observational study was evaluate changes patients undergoing elective joint surgery under general (GA) vs. neuraxial (NA). Our hypothesis that will be lower NA.Sixty...
Liver resection is a surgical procedure associated with high risk of hepatic failure that can be fatal. One the key mechanisms involves ischemia-reperfusion damage. Building on well-known positive effects hydrogen at mitigating this damage, goal work was to demonstrate antioxidant, anti-inflammatory, and anti-apoptotic inhaled in domestic pigs during major liver resection.The study used total 12 pigs, 6 animals underwent general anesthesia, same using conventional, unsupplemented,...
BACKGROUND: Endothelial glycocalyx (EG) is a carbohydrate-rich gel-like mesh covering the apical surface of endothelial cells. It has been linked to microvascular pathophysiology and tissue metabolism. However, little known about its condition in young healthy adults. OBJECTIVE: We aimed describe EG adults by vivo imaging measurement syndecan-1, plasma marker integrity order obtain reference values. METHODS: For studies we used Side-stream Dark Field sublingual microcirculation. Recordings...
BACKGROUND: Endothelial glycocalyx (EG) plays a crucial role in maintaining the plasma proteins within intravascular space. OBJECTIVE: We studied whether exogenous albumin protects EG an experimental model of enzymatic damage rats. METHODS: Rats were divided into three groups 10 animals that received (1) Evans blue (2) + hyaluronidase, or (3) hyaluronidase 20% human via tail vein. Spectrophotometric analysis was performed 2 h later to quantify leakage blue-labeled heart, lungs, brain,...
Introduction: Surgical treatment is associated with an unwanted response of the organism to so-called surgical trauma. This called stress. Ischaemia-reperfusion injury one essential causes tissue damage. It comprises functional and structural changes in that occur after restoration circulation, episode ischaemia. Necrosis irreversibly changed cells endothelial mitochondrial- induced swelling occur. Methods: Physiology, pathophysiology glycocalyx: Endothelial glycocalyx a 0.2 5 micrometres...
The endothelial glycocalyx (EG) is fragile and sensitive to damage such as exposure hypernatremia. Our aim was describe the influence of hypernatremia on EG in sublingual brain microcirculation rabbits.Hypernatremia induced by intravenous administration 10% NaCl solution. were evaluated Side-stream Dark Field imaging before (T1) 20 minutes after infusion saline (T2). Damage quantified automated analysis Perfused Boundary Region (PBR) indicating amount penetration red blood cells into EG....
Background: Fluid loading and hyperosmolar solutions can modify the cortical brain microcirculation endothelial glycocalyx (EG). This study compared short-term effects of liberal fluid with a restrictive intake followed by osmotherapy hypertonic saline (HTS) on cerebral EG integrity in rabbit craniotomy model. Methods: The experimental rabbits were allocated randomly to receive either <2 mL/kg/h (group R, n=14) or 30 L, balanced isotonic fluids for 1 hour. Then, animals randomized 5 mL/kg...