A5000757040- Nitric Oxide and Endothelin Effects
- Renin-Angiotensin System Studies
- Protease and Inhibitor Mechanisms
- NF-κB Signaling Pathways
- Dark Matter and Cosmic Phenomena
- Alzheimer's disease research and treatments
- Cytokine Signaling Pathways and Interactions
- Inflammatory mediators and NSAID effects
- Particle physics theoretical and experimental studies
- Parkinson's Disease Mechanisms and Treatments
- Particle Detector Development and Performance
- Autophagy in Disease and Therapy
- Ion channel regulation and function
- Osteoarthritis Treatment and Mechanisms
- Enzyme Production and Characterization
- Receptor Mechanisms and Signaling
- Diabetes and associated disorders
- Birth, Development, and Health
- Congenital heart defects research
- Cardiac Fibrosis and Remodeling
- Protein Kinase Regulation and GTPase Signaling
- Genetics and Neurodevelopmental Disorders
- Lipid metabolism and biosynthesis
- Radiation Detection and Scintillator Technologies
- Quantum Chromodynamics and Particle Interactions
Osaka Institute of Technology
2025
The University of Tokyo
1992-2024
Daiichi-Sankyo (Japan)
1996-2016
Juntendo University
2008-2014
Daiichi Sankyo (Germany)
1992-2013
Tokyo Metropolitan Geriatric Hospital
2013
Hokkaido University
2007-2012
Tenshi College
2012
Obihiro University of Agriculture and Veterinary Medicine
2012
Kyoto Prefectural University of Medicine
2012
Abstract The MEG II experiment, based at the Paul Scherrer Institut in Switzerland, reports result of a search for decay $$\upmu ^+ \rightarrow {\textrm{e}}^+ \upgamma $$ <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"> <mml:mrow> <mml:msup> <mml:mi>μ</mml:mi> <mml:mo>+</mml:mo> </mml:msup> <mml:mo>→</mml:mo> <mml:mtext>e</mml:mtext> </mml:mrow> <mml:mi>γ</mml:mi> </mml:math> from data taken first physics run 2021. No excess events over expected background is observed, yielding an...
Endothelin (ET) is a 21-residue potent vasoconstrictive peptide produced by vascular endothelial cells and formed from its precursor, big endothelin (big ET), endothelin-converting enzyme (ECE).Here we report the cloning functional expression of complementary DNA encoding rat ECE cells.Rat highly glycosylated protein consisting 10 possible N-linked glycosylation sites, zinc-binding domain, single membrane-spanning region.It has structural sequence homology to neutral endopeptidase Kell blood...
Abstract The MEG II experiment, located at the Paul Scherrer Institut (PSI) in Switzerland, is successor to which completed data taking 2013. started fully operational 2021, with goal of improving sensitivity $$\upmu ^+ \rightarrow {\textrm{e}}^+ \upgamma $$ <mml:math...
Background —Elevated plasma levels of endothelin (ET)-1 have been reported in association with heart diseases, including failure. Furthermore, it has suggested that ET-1 acts as a local autocrine/paracrine factor biological activities such vasoconstriction, mitogenesis, and inotropic effects on the heart. This study investigated alterations ET-1, ET receptor, endothelin-converting enzyme (ECE) expression left ventricular myocardium from patients end-stage Methods Results —mRNA concentrations...
Background Perilipin A (PeriA) exclusively locates on adipocyte lipid droplets and is essential for storage lipolysis. Previously, we reported that specific overexpression of PeriA caused resistance to diet-induced obesity resulted in improved insulin sensitivity. In order better understand the biological basis this observed phenotype, performed additional studies transgenic mouse model. Methodology Principal Findings When compared control animals, whole body energy expenditure was increased...
We investigated the role of periostin, an extracellular matrix protein, in pathophysiology osteoarthritis (OA). In OA, dysregulated gene expression and phenotypic changes articular chondrocytes culminate progressive loss cartilage from joint surface. The molecular mechanisms underlying this process are poorly understood. examined periostin by immunohistochemical analysis lesional nonlesional human rodent OA knee cartilage. addition, we used small interfering (si) RNA adenovirus transduction...
Background Endothelin 1 has been implicated in various human diseases, including atherosclerosis. In this study, we examined the expression and localization of endothelin-converting enzyme-1 (ECE-1), final key enzyme endothelin processing, rat carotid arteries after balloon injury coronary atherosclerotic lesions. Methods Results ECE-1 mRNA levels ECE activity balloon-injured started to increase between 2 5 days injury. The content tissue injured was concomitantly increased....
We report the cloning and sequencing of 5'-terminal region a beta form rat ECE-1 cDNA which is different only in its N-terminal amino-acid sequence to we have cloned previously (alpha [K. Shimada et al. (1994) J. Biol. Chem. 269, 18275-18278]). No significant difference was found specific activity substrate specificity between two isoforms. The expression level alpha mRNA higher than that various cells tissues, suggesting physiologically important isoform alpha. present findings verified...
Endothelin is a potent vasoconstrictive peptide that produced by vascular endothelial cells; it formed from its precursor, big endothelin, endothelin-converting enzyme (ECE). In this work, ECE was studied using specific monoclonal antibodies. immunoblotting, estimated to be 300 kDa protein on SDS/PAGE under non-reducing conditions, and 130 reducing conditions. Cross-linking experiments revealed composed of two disulphide-linked subunits. Localization at the cellular subcellular levels in...
Endothelin-converting enzyme-1 (ECE-1) is involved in the conversion of big endothelins (big ETs) into (ETs) and shows sequence similarity with neutral endopeptidase-24.11 (NEP). Unlike NEP, ECE-1 exists as a disulphide-linked dimer. Here we reveal that Cys412 solely responsible for dimerization rat ECE-1. The C412S mutant enzyme, which existed monomer, showed no difference glycosylation level, subcellular localization or clustering structure formation, but higher Km lower kcat ET-1 compared...
The MEG experiment took data at the Paul Scherrer Institute in years 2009–2013 to test violation of lepton flavor conservation law, which originates from an accidental symmetry that Standard Model elementary particle physics has, and published most stringent limit on charged violating decay μ+→e+γ: BR(μ+→e+γ) <4.2×10−13 90% confidence level. detector has been upgraded order reach a sensitivity 6×10−14. basic principle II is achieve highest possible using full muon beam intensity (7×107...
Prostaglandin E 2 (PGE ) is well known as a mediator of inflammatory symptoms such fever, arthritis, and pain. In the present study, we evaluated analgesic effect our selective PGE synthesis inhibitor, compound I, 2-methyl-2-[ cis -4-([1-(6-methyl-3-phenylquinolin-2-yl)piperidin-4-yl]carbonyl amino)cyclohexyl] propanoic acid, in rat yeast-induced acute adjuvant-induced chronic pain models. Although this suppressed selectively, no was shown both Prostacyclin (PGI also plays crucial roles...
To investigate a series of new inhibitors gelatinases based on matlystatin B (1b), extensive structure-activity relationship studies were performed. The derivatives evaluated in vitro for the ability to inhibit gelatinases. inhibitory activities against thermolysin also assayed test compounds' selectivity. Among compounds modified at P'3 moiety, N-methylamide derivative 5 g was virtually twice as effective gelatinase parent compound 1b (5g, IC50 = 0.27 microM vs. 1b, 0.57 microM). Other...
Endothelin-converting enzyme is a phosphoramidon-sensitive membrane metallopeptidase that catalyses the final step in biosynthesis of potent vasoactive endothelin peptides. Immunomagnetic separation technology and immunohistochemistry have been used to demonstrate co-localisation endothelin-converting with established ectoenzyme, aminopeptidase N, on surface endothelial cells. Unlike however, seen associate clusters plasma which can be distinguished from caveolae both biochemically...
Abstract Background Proinsulin C‐peptide shows ameliorative effects on diabetic complications, possibly through the production of nitric oxide (NO). On contrary, increased local availability NO and expression endothelial synthase (eNOS) in renal endothelium are shown to be involved progression nephropathy. The aim this study was elucidate effect insulin as a reference eNOS early phase type 1 rat kidney. Methods Type diabetes rats produced by streptozotocin injection some were treated with...