A5100748429- Mesenchymal stem cell research
- Advanced biosensing and bioanalysis techniques
- Periodontal Regeneration and Treatments
- Ocular Diseases and Behçet’s Syndrome
- Luminescence and Fluorescent Materials
- Nanoplatforms for cancer theranostics
- Advanced Biosensing Techniques and Applications
- Porphyrin and Phthalocyanine Chemistry
- Immunotherapy and Immune Responses
- Bone and Dental Protein Studies
- RNA Interference and Gene Delivery
- Tissue Engineering and Regenerative Medicine
- Fibroblast Growth Factor Research
- Tryptophan and brain disorders
- Monoclonal and Polyclonal Antibodies Research
- Gut microbiota and health
- Systemic Lupus Erythematosus Research
- Kawasaki Disease and Coronary Complications
- Neuroinflammation and Neurodegeneration Mechanisms
- Autophagy in Disease and Therapy
- Coronary Artery Anomalies
- Biosensors and Analytical Detection
- Single-cell and spatial transcriptomics
- Molecular Sensors and Ion Detection
- Inflammasome and immune disorders
University of Science and Technology Beijing
2016-2025
Beijing Children’s Hospital
2016
Capital Medical University
2016
NorthShore University HealthSystem
2010-2011
Center for Clinical Research (United States)
2010
Tsinghua University
2004-2005
Peking University
2004
A simple, highly sensitive, and selective multiple microRNA (miRNA) detection method based on the graphene oxide (GO) fluorescence quenching isothermal strand-displacement polymerase reaction (ISDPR) was proposed. The capability to discriminate ssDNA double-stranded nucleic acid structure coupled with extraordinary of GO organic dye allows proposed strategy simultaneously selectively detect several miRNA labeled different dyes in same solution, while ISDPR amplification endows high...
Controlled release system based on mesoporous silica (MS) nanomaterials has drawn great attention over the past decades due to its potential biomedical applications. Herein, a light-responsive MS nanoparticles was achieved by adjusting wetting of surface. At starting stage, surface modified with optimal ratio spiropyran fluorinated silane (MS-FSP) protected from being wetted water, successfully inhibiting model cargo molecules, fluorescein disodium (FD). Upon irradiation 365 nm UV light,...
Chlorogenic acid, an important active component of coffee with anti-tumor activities, has been found for a hundred years. However, the lack understanding about its target proteins greatly limits exploration molecular mechanisms and clinical applications. Here, in vitro animal experiments showed that chlorogenic acid had significant inhibitory effect on proliferation A549 cells. The ability to naturally emit fluorescence was exploited screen while avoiding false positives brought by chemical...
Abstract A grade 3 burn is a nonstatic fatal injury, which can lead to complete damage the skin structure, accompanied by series of symptoms such as persistent inflammation, pain, pruritus, ulcer, and peripheral neuropathy. Although primary clinical treatment grafting, it cannot comprehensively solve symptoms. Here, multifunctional DNA hydrogel integrated system conveniently obtained through dynamic cross‐linking unit, polyacrylamide, l ‐ascorbate 2‐phosphate ( ‐A2P) formation dense hydrogen...
Abstract Single-cell sequencing is frequently affected by “omission” due to limitations in throughput, yet bulk RNA-seq may contain these ostensibly “omitted” cells. Here, we introduce the single cell trajectory blending from Bulk (BulkTrajBlend) algorithm, a component of OmicVerse suite that leverages Beta-Variational AutoEncoder for data deconvolution and graph neural networks discovery overlapping communities. This approach effectively interpolates restores continuity cells within...
Objective IgG4-related disease (IgG4-RD) is a chronic systemic involved in many organs and tissues. As only limited autoantigens have been found since the beginning of this century, aim study was to reveal new candidate IgG4-RD. Methods Multiple cell lines including HT-29, EA.hy926, HEK 293 HepG2 were used test binding ability circulating autoantibodies from IgG4-RD sera. The amino-acid sequence then analyzed by matrix-assisted laser desorption/ionization time-of-flight tandem...
Non-alcoholic fatty liver disease (NAFLD) is the most common among lipid metabolism disorders. Autophagy plays an important role in NAFLD. Pueraria flavonoids, main active ingredients of lobata, exert antioxidant and anti-inflammatory effects. Herein, we report potential lipid-lowering effects flavonoids on NAFLD induced by a high-fat diet. In vivo vitro experiments showed that reduced intracellular deposition inhibiting synthesis release pro-inflammatory cytokines. We analyzed autophagy...
Here we report a controllable method based on electrodeposition to fabricate Ag nanodendrites (NDs) microwell patterned electrode. The patterns the ITO electrode are fabricated via microcontact printing technique. By varying size and time, morphology of metal deposits can be tuned from boulders dendrites. At edge microwells, current density was strengthened, which incurs rapid nucleation. nucleus develops into dendrites because Mullins–Sekerka instability. However, only were observed at...
BODIPY-based ratiometric fluorescent probes show remarkable sensitivity under acidic conditions <italic>via</italic> a protonation-modulated ICT mechanism for monitoring lysosomal pH in living cells.
DNA assembles that can perform "OR" and "AND" logic gate operations were fabricated. The feasibility of intelligent controlled release was demonstrated through the organization gold nanoparticles on surface mesoporous silica by stimuli-induced structural transformation ensembles.
Two-photon excitable fluorescent dyes with integrated functions of targeted imaging and photodynamic therapy (PDT) are highly desired for the development cancer theranostic agents. Herein, fluorescence resonance energy transfer (FRET) dyads, AceDAN-H2Por-Lyso (1a) AceDAN-ZnPor-Lyso (1b), were developed two-photon excited (TPE) lysosome-targeted PDT cells. Under one-photon or excitation, AceDAN donor can effectively state to porphyrin acceptor via high efficient FRET, leading generation...
Human gingival mesenchymal stem cells (GMSCs) are derived from migratory neural crest and have the potential to differentiate into neurons. Metformin can inhibit stem-cell aging promotes regeneration development of In this study, we investigated metformin as an enhancer on neuronal differentiation GMSCs in growth environment chitosan hydrogel. The crosslinked chitosan/β-glycerophosphate hydrogel form a perforated microporous structure that is suitable for cell channels transport water...
An intelligent photoswitchable single-molecule nanomachine with DNA hairpin-loop structure was designed by the incorporation of azobenzene groups in sequences, which studied fluorescence resonance energy transfer (FRET) and attached onto surface mesoporous silica. Based on photo-induced conformational transformation DNA, highly efficient controlled release realized.
Fecal microbiota transplant (FMT) is a potential treatment approach for many diseases. Alzheimer's disease (AD) and cancer have been proven to specific antagonistic relationship FMT.This article aims explore whether intestinal flora transplantation from individuals can ameliorate cognitive impairment.Morris water maze object recognition tests were performed assess function after the fecal tumor-bearing WT mice transplanted into AD by gavage. The effect of on was analyzed thioflavin T...
Harmful cyanobacterial blooms in waters have become a global environmental problem, this mainly due to the production and release of various microalgal toxins, which microcystins (MCs) are distributed widely. Here, we focused on study typical form called microcystin-YR (MC-YR). It was found that initial 14.8 mg/L MC-YR could be completely eliminated within 10 hr by crude enzymes (CEs) Sphingopyxis sp. USTB-05, promising bacterial strain isolated identified our previous study. During...