A5102918964- Algal biology and biofuel production
- Genomics and Phylogenetic Studies
- Microbial Metabolic Engineering and Bioproduction
- Photosynthetic Processes and Mechanisms
- Ginseng Biological Effects and Applications
- RNA and protein synthesis mechanisms
- Plant Molecular Biology Research
- Cancer-related molecular mechanisms research
- Enzyme Catalysis and Immobilization
- Antioxidant Activity and Oxidative Stress
- Molecular Biology Techniques and Applications
- Microbial Community Ecology and Physiology
- RNA Research and Splicing
- RNA modifications and cancer
- Phytochemicals and Antioxidant Activities
- Agriculture, Soil, Plant Science
- Viral Infectious Diseases and Gene Expression in Insects
- Genomics and Rare Diseases
- Traditional Chinese Medicine Analysis
- CRISPR and Genetic Engineering
- Gut microbiota and health
- Metabolomics and Mass Spectrometry Studies
- Fatty Acid Research and Health
- Plant responses to water stress
- Chromatography in Natural Products
China Agricultural University
2025
Northwest A&F University
2019-2025
Jilin Agricultural University
2019-2024
Yunnan Agricultural University
2024
Fujian Agriculture and Forestry University
2022-2024
East China University of Science and Technology
2021-2024
Korea Research Institute of Bioscience and Biotechnology
2024
Chonnam National University
2024
Nanjing University of Chinese Medicine
2020-2023
Sun Yat-sen University
2023
Abstract Long-read sequencing is promising for the comprehensive discovery of structural variations (SVs). However, it still non-trivial to achieve high yields and performance simultaneously due complex SV signatures implied by noisy long reads. We propose cuteSV, a sensitive, fast, scalable long-read-based detection approach. cuteSV uses tailored methods collect various types SVs employs clustering-and-refinement method implement sensitive detection. Benchmarks on simulated real long-read...
Photosynthetic microalgae are emerging as potential biomass feedstock for sustainable production of biofuels and value-added bioproducts. CO2 biomitigation through these organisms is considered an eco-friendly promising alternative to the existing carbon sequestration methods. Nonetheless, inherent relatively low photosynthetic capacity has hampered practical use this strategy applications. Here, we demonstrate feasibility improving by genetic manipulation Calvin cycle in typical green...
Metatranscriptomics is emerging as a powerful technology for the functional characterization of complex microbial communities (microbiomes). Use unbiased RNA-sequencing can reveal both taxonomic composition and active biochemical functions community. However, lack established reference genomes, computational tools pipelines make analysis interpretation these datasets challenging. Systematic studies that compare data across microbiomes are needed to demonstrate ability such deliver...
Abstract Motivation: The development of high-throughput sequencing technologies has enabled novel methods for detecting structural variants (SVs). Current are typically based on depth coverage or pair-end mapping clusters. However, most these only report an approximate location each SV, rather than exact breakpoints. Results: We have developed pair-read informed split (PRISM), a method that identifies SVs and their precise breakpoints from whole-genome resequencing data. PRISM uses...
Since the establishment of first biomedical ontology Gene Ontology (GO), number has increased dramatically. Nowadays over 300 ontologies have been built including extensively used Disease (DO) and Human Phenotype (HPO). Because advantage identifying novel relationships between terms, calculating similarity terms is one major tasks in this research area. Though similarities within each studied with silico methods, term across different were not investigated as deeply. The latest method took...
Group I and II introns self-splice in vitro , but require proteins for efficient splicing vivo to stabilize the catalytically active RNA structure. Recent studies showed that of some Neurospora crassa mitochondrial group additionally requires a DEAD-box protein, CYT-19, which acts as an chaperone resolve nonnative structures formed during folding. Here we show that, Saccharomyces cerevisiae mitochondria, related Mss116p, is required all introns, end-processing reactions, translation subset...
Lipid class composition and distribution of fatty acids within the lipid pool microalga, Schizochytrium mangrovei FB3 harvested at late exponential phase, was studied, with special emphasis on docosahexaenoic acid (C22:6 n-3, DHA). Neutral lipids were major constituents (95.90% total lipids) in which triacylglyerol (TAG) predominant component accounted for 97.20% neutral lipids. Phosphatidylcholine (PC) polar lipid. Phosphatic phosphatidylserine two classes phospholipids reported first time...
Extending the carotenoid pathway to astaxanthin in plants is of scientific and industrial interest. However, expression a microbial β-carotene ketolase (BKT) that catalyses formation ketocarotenoids transgenic typically results low levels astaxanthin. The efficiency BKTs ketolating zeaxanthin proposed be major limitation for accumulation engineered plants. To verify this hypothesis, several algal were functionally characterized using an Escherichia coli system three identified, with high (up...
With the development of High-Throughput Sequencing (HTS) thousands human genomes have now been sequenced. Whenever different studies analyze same genome they usually agree on amount single-nucleotide polymorphisms, but differ dramatically number insertion and deletion variants (indels). Furthermore, there is evidence that indels are often severely under-reported. In this manuscript we derive total indel in a by combining data from sequencing technologies, while assessing detection accuracy....
The similarity of pair-wise diseases reveals the molecular relationships between them. For example, similar have potential to be treated by common therapeutic chemicals (TCs). In this paper, we introduced DisSim, an online system for exploring diseases, and comparing corresponding TCs. Currently, DisSim implemented five state-of-the-art methods measure Disease Ontology (DO) terms provide significance score. Furthermore, integrated TCs from Comparative Toxicogenomics Database (CTD), which can...
Abstract The C2H2 zinc finger protein (C2H2-ZFP) gene family plays important roles in response to environmental stresses and several other biological processes plants. Ginseng is a precious medicinal herb cultivated Asia North America. However, little known about the C2H2-ZFP its functions ginseng. Here, we identified 115 genes from ginseng, defined as PgZFP family. It was clustered into five groups featured with eight conserved motifs, each containing one six of them. are categorized 17...
Abstract The effects of temperature and shift on the fatty acid composition docosahexaenoic (DHA, C22∶6n−3) content productivity marine microalga Crypthecodinium cohnii ATCC 30556 were investigated. grew well over entire range temperatures (15–30°C) studied. High favored growth with highest specific rate 0.092 h −1 at 30°C. In contrast, low formation polyunsaturated acids. DNA was obtained 15°C in early stationary phase (i.e., 72h). order to achieve high DHA productivity, a from later stage...