A5007372033- Aquaculture Nutrition and Growth
- Aquaculture disease management and microbiota
- Parasite Biology and Host Interactions
- Helminth infection and control
- Molecular Biology Techniques and Applications
- Reproductive biology and impacts on aquatic species
- Uterine Myomas and Treatments
- Parasites and Host Interactions
- Hematopoietic Stem Cell Transplantation
- Physiological and biochemical adaptations
- Cancer Genomics and Diagnostics
- Lipid metabolism and biosynthesis
- Marine Ecology and Invasive Species
- Genetic diversity and population structure
- Zebrafish Biomedical Research Applications
- Endometrial and Cervical Cancer Treatments
- Circadian rhythm and melatonin
- Ichthyology and Marine Biology
- Glutathione Transferases and Polymorphisms
- RNA Interference and Gene Delivery
- Endometriosis Research and Treatment
- Nematode management and characterization studies
- HIV/AIDS drug development and treatment
- Marine and fisheries research
- Genetics, Aging, and Longevity in Model Organisms
University of Stirling
2011-2024
Instituto de Medicina Genómica
2018-2023
Parc Científic de la Universitat de València
2018-2020
Abstract Docosahexaenoic acid (DHA) plays important physiological roles in vertebrates. Studies rats and rainbow trout confirmed that DHA biosynthesis proceeds through the so-called “Sprecher pathway”, a biosynthetic process requiring Δ6 desaturation of 24:5n−3 to 24:6n−3. Alternatively, some teleosts possess fatty acyl desaturases 2 (Fads2) enable them more direct route termed “Δ4 pathway”. In order elucidate prevalence both pathways among teleosts, we investigated ability towards C 24...
The ability to produce physiologically critical LC-PUFA from dietary fatty acids differs greatly among teleost species, and is dependent on the possession expression of acyl desaturase elongase genes. Atlantic salmon, as a result recently duplicated genome, have more these enzymes than other fish. Recent phylogenetic studies show that Northern pike represents closest extant relative preduplicated ancestral salmonid. Here we characterise elongase, elovl5, compare it salmon elovl5a elovl5b...
Rainbow trout, Oncorhynchus mykiss, are intensively cultured globally. Understanding their requirement for long-chain polyunsaturated fatty acids (LC-PUFA) and the biochemistry of enzymes biosynthetic pathways required acid synthesis is important highly relevant in current aquaculture. Most gnathostome vertebrates have two desaturase (fads) genes with known functions LC-PUFA biosynthesis termed fads1 fads2. However, teleost fish exclusively fads2 genes. In rainbow a cDNA had been previously...
Background The analysis of molecular haematopoietic chimerisms (HC) has become a well-established method to monitor the transplant evolution and assess risk relapse after allogeneic stem cells transplantation (allo-STC). Different techniques markers are being used for chimerism surveillance transplantation, including quantitative real-time PCR (qPCR) recently developed digital (dPCR). This study aims compare sensitivity accuracy both methods quantify HC predict early relapse. Methodology was...
Parasitic infections by the salmon louse, Lepeophtheirus salmonis (Krøyer), cause huge economic damage in farming northern hemisphere, with combined treatment costs and production losses 2014 having been estimated at US$ 350 million for Norway (annual 1.25 tonnes). The control of L. relies significantly on medicinal treatments, supplemented non-pharmacological approaches. However, efficacy have reported several delousing agents, including pyrethroid deltamethrin. aim present study was to...
Circadian regulation of hepatic detoxification seems to be amongst the key roles biological clock. The liver is major site for biotransformation, and in mammals, it contains several clock-controlled transcription factors such as proline acidic amino acid-rich basic leucine zipper proteins (PAR bZIP) basic-helix-loop-helix Per-Arnt-Sim (bHLH-PAS) family that act circadian regulators genes. This investigation explored existence daily expression involved detoxification, well temporal profile a...
Salmon lice, Lepeophtheirus salmonis (Krøyer, 1837), are fish ectoparasites causing significant economic damage in the mariculture of Atlantic salmon, Salmo salar Linnaeus, 1758. The control L. at farms relies to a large extent on treatment with anti-parasitic drugs. A problem related chemical is potential for development resistance, which documented number drug classes including organophosphates, pyrethroids and avermectins. ATP-binding cassette (ABC) gene superfamily found all biota...
Ethanol is one of the most commonly abused drugs and consequently its toxic psychoactive effect has been widely investigated, although little known about time-dependent effects this drug. In present research zebrafish was used to assess daily rhythms in ethanol toxicity behavioural effects, as well temporal pattern expression key genes involved detoxification liver (adh8a, adh5, aldh2.1 aldh2.2). Our results showed marked differences mortality rate larvae depending on time day exposure 5%...
The salmon louse (Lepeophtheirus salmonis) infests farmed and wild salmonid fishes, causing considerable economic damage to the farming industry. Infestations of are controlled using a combination non-medicinal approaches veterinary drug treatments. While L. salmonis has developed resistance most available delousing agents, relatively little is known about molecular mechanisms involved. Members cytochrome P450 (CYP) superfamily typically monooxygenases, some which involved in biosynthesis...
The salmon louse Lepeophtheirus salmonis (Krøyer, 1837) is an ectoparasite causing infections of wild and farmed Atlantic (Salmo salar L.) in the Northern hemisphere. While L. control at commercial mariculture sites increasingly employs non-medicinal approaches, such as cage designs reducing infection rates biological through cleaner fish, anti-parasitic drugs are still a requirement for effective fish health care. With only limited range delousing agents available, all which have been use...
Parasitic salmon lice (Lepeophtheirus salmonis) cause high economic losses in Atlantic farming. Pyrethroids, which block arthropod voltage-gated sodium channels (Nav 1), are used for delousing. However, pyrethroid resistance is common L. salmonis. The present study characterized Nav 1 homologues salmonis order to identify channel mutations associated resistance, called kdr (knockdown) mutations.Genome scans identified three homologues, LsNav 1.1, 1.2 and 1.3. Arthropod map specific regions...
The population composition and aspects of the reproductive biology 452 blackmouth catsharks, Galeus melastomus were investigated. sharks incidentally caught off North-Western Aegean, Eastern Corinthian Northern Euboean Gulfs by commercial bottom trawlers longlines at depths between 200 500 m. overall sex ratio (1:1.1 females:males) was not different from theoretical unit (1:1). No differences in ratios detected among seasons, areas or depth strata. Significant structure regarding maturity...
Abstract BACKGROUND The pyrethroid deltamethrin is used to treat infestations of farmed salmon by parasitic lice, Lepeophtheirus salmonis (Krøyer). However, the efficacy for delousing threatened resistance development. In terrestrial arthropods, knockdown ( kdr ) mutations voltage‐gated sodium channel (Na v ), molecular target pyrethroids, can cause resistance. A putative mutation an L. homologue (LsNa 1.3 I936V) has been identified previously. At same time, shown be inherited maternally and...
Abstract Background The salmon louse ( Lepeophtheirus salmonis ) is a parasite of wild and farmed salmonid fish, causing huge economic damage to the commercial farming Atlantic Salmo salar in northern hemisphere. avermectin emamectin benzoate (EMB) widely used for delousing. While resistance EMB widespread populations L. , molecular mechanisms remain be elucidated. aim present work was obtain insights into potential by identifying genetic transcriptomic markers associated with resistance....