A5039610620- Lipid metabolism and biosynthesis
- Peroxisome Proliferator-Activated Receptors
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Star Cryoelectronics (United States)
2024
Washington University in St. Louis
2009-2022
Great Ormond Street Hospital
2021
University College London
2021
Indiana University School of Medicine
2009
Indiana University – Purdue University Indianapolis
2009
University of Minnesota
2001-2006
University of York
2003
University of Oklahoma
1998
Tabor College
1976
Fatty acid transport protein 1 (FATP1) is an ∼63-kDa plasma membrane that facilitates the influx of fatty acids into adipocytes as well skeletal and cardiac myocytes. Previous studies with FATP1 expressed in COS1 cell extracts suggested exhibits very long chain acyl-CoA synthetase (ACS) activity such may be linked to transport. To address enzymatic isolated protein, murine ACS1 were engineered contain a C-terminal Myc-His tag cells via adenoviral-mediated infection purified homogeneity using...
Fatty acid transport protein 4 (FATP4) is an integral membrane expressed in the plasma and internal membranes of small intestine adipocyte as well brain, kidney, liver, skin, heart. FATP4 has been hypothesized to be bifunctional, exhibiting both fatty acyl-CoA synthetase activities that work concert mediate influx across biological membranes. To determine whether synthetase, murine was engineered contain a C-terminal FLAG epitope tag, COS1 cells via adenovirus-mediated infection purified...
The role of fatty acid transport protein 1 (FATP1) and FATP4 in facilitating adipocyte metabolism was investigated using stable FATP1 or knockdown (kd) 3T3-L1 cell lines derived from retrovirus-delivered short hairpin RNA (shRNA). Decreased expression did not affect preadipocyte differentiation the (in kd), translocase, acyl-coenzyme A synthetase 1, binding but lead to increased levels peroxisome proliferator-activated receptor gamma CCAAT/enhancer alpha. Both kd adipocytes exhibited reduced...
Leptin plays an important role in regulating energy expenditure response to food intake, but nutrient regulation of leptin is incompletely understood. In this study using vivo and vitro approaches, we examined the fatty acid uptake modulating expression production. levels are doubled CD36-null mouse, which has impaired cellular despite a 40% decrease fat mass. The mouse protected from diet-induced weight gain not that consequent deficiency. secretion strongly responsive glucose whereas...
Although there appear to be no differences in muscle protein turnover young and middle aged men women, we have reported significant the rate of synthesis between older adult women. This suggests that aging may affect differently women.We measured skeletal fractional (FSR) by using stable isotope-labeled tracer methods during basal postabsorptive conditions a hyperaminoacidemic-hyperinsulinemic-euglycemic clamp eight (25-45 y), ten women old (65-85 y) y).The FSR was not different (0.040 ±...
Intrahepatic lipid accumulation is extremely common in obese subjects and associated with the development of insulin resistance diabetes. Hepatic diacylglycerol triacylglycerol synthesis predominantly occurs through acylation glycerol-3-phosphate. However, an alternative pathway for synthesizing from monoacylglycerol acyltransferases (MGAT) could also contribute to hepatic glyceride pools. MGAT activity expression three genes encoding enzymes (MOGAT1, MOGAT2, MOGAT3) were determined liver...
Lipin 1 is a bifunctional intracellular protein that regulates fatty acid metabolism in the nucleus via interactions with DNA-bound transcription factors and at endoplasmic reticulum as phosphatidic phosphohydrolase enzyme (PAP-1) to catalyze penultimate step triglyceride synthesis. However, livers of 8-day-old mice lacking lipin (fld mice) exhibited normal PAP-1 activity 20-fold increase levels. We sought further analyze hepatic lipid profile these by electrospray ionization mass...
Monoacylglycerol acyltransferase (MGAT) enzymes convert monoacylglycerol to diacylglycerol (DAG), a lipid that has been linked the development of hepatic insulin resistance through activation protein kinase C (PKC). The expression genes encode MGAT is induced in livers insulin-resistant human subjects with nonalcoholic fatty liver disease, but whether causal steatosis or unknown. We show Mogat1, which encodes MGAT1, and activity are also increased diet-induced obese (DIO) ob/obmice. To probe...
Lipin 1 is a coregulator of DNA-bound transcription factors and phosphatidic acid (PA) phosphatase (PAP) enzyme that catalyzes critical step in the synthesis glycerophospholipids. highly expressed adipocytes, constitutive loss lipin blocks adipocyte differentiation; however, effects Lpin1 deficiency differentiated adipocytes are unknown. Here we report adipocyte-specific gene recombination unexpectedly resulted expression truncated protein lacking PAP activity but retaining transcriptional...
Biosynthesis of lipids was investigated in growing 293 cells stably expressing fatty acid (FA) transport protein 1 (FATP1), a bifunctional polypeptide with FA as well acyl-CoA synthetase activity. In short-term (30 s) incubations, uptake increased FATP1 (C8 cells) compared the vector (as determined by BODIPY 3823 staining and radioactive uptake). long-term (4 h) incorporation [14C]acetate, [3H]oleic acid, or [14C]lignoceric into 1,2,3-triacyl-sn-glycerol (TG) elevated C8 vector, whereas...
Lipid droplet proteins (LDPs) coat the surface of triglyceride-rich lipid droplets and regulate their formation lipolysis. We profiled hepatic LDP expression in fatty liver dystrophic (fld) mice, a unique model neonatal steatosis that predictably resolves between postnatal day 14 (P14) P17. Western blotting revealed perilipin-2/ADRP perilipin-5/OXPAT were markedly increased steatotic fld but returned to normal by However, changes perilipin-2 perilipin-5 protein content mice exaggerated...
ABSTRACT The ligand-gated outer membrane porin FepA serves Escherichia coli as the receptor for siderophore ferric enterobactin. We characterized ability of seven analogs enterobactin to supply iron via by quantitatively measuring binding and transport their 59 Fe complexes. experiments refuted idea that chirality complex affects its recognition demonstrated necessity an unsubstituted catecholate coordination center protein. Among compounds we tested, only enantioenterobactin, synthetic,...
Abnormalities in hepatic lipid metabolism and insulin action are believed to play a critical role the etiology of nonalcoholic steatohepatitis. Monoacylglycerol acyltransferase (MGAT) enzymes convert monoacylglycerol diacylglycerol, which is penultimate step one pathway for triacylglycerol synthesis. Hepatic expression Mogat1, encodes an MGAT enzyme, increased livers mice with steatosis, knocking down Mogat1 improves glucose signaling, but whether activity plays steatohepatitis unclear. To...
Adipocyte triglyceride storage provides a reservoir of energy that allows the organism to survive times nutrient scarcity, but excessive adiposity has emerged as health problem in many areas world. Monoacylglycerol acyltransferase (MGAT) acylates monoacylglycerol produce diacylglycerol; penultimate step synthesis. However, little is known about MGAT activity adipocytes, which are believed rely primarily on another pathway for We show expression gene encodes MGAT1 robustly induced during...
The hepatic expression of the cell death-inducing DNA fragmentation factor A-like effector family (CIDEA, CIDEB, and CIDEC) genes is markedly upregulated in mouse models obesity. We evaluated CIDE liver obese human subjects undergoing gastric bypass surgery (GBS), at time again 1 year later when had lost 37.6 +/- 1.4% their initial body weight. At GBS, CIDEA (r(2) = 0.20, P 0.04) CIDEC 0.32, 0.01) was strongly correlated with BMI, whereas CIDEB not 0.01, 0.81). One after surgery, declined...
The prevalence of obesity‐associated nonalcoholic fatty liver disease has significantly increased over the past decade, and end‐stage secondary to steatohepatitis become 1 most common indications for transplantation. This both increases demand organs decreases availability donor livers deemed suitable Although in many steatotic were discarded due concerns enhanced susceptibility ischemia/reperfusion injury (IRI) organ failure, discrepancy between supply resulted increasing use expanded...
Nonalcoholic fatty liver disease (NAFLD) is becoming the most common indication for transplantation. The growing prevalence of NAFLD not only increases demand transplantation, but it also limits supply available organs because steatosis predisposes grafts to ischemia/reperfusion injury (IRI) and many steatotic are discarded. We have shown that monoacylglycerol acyltransferase (MGAT) 1, an enzyme converts diacylglycerol, highly induced in animal models patients with important mediator...
Journal Article Thin-layer chromatography of corticosteroids Get access A Hall Glaxo Laboratories Limited, Montrose, Angus Search for other works by this author on: Oxford Academic Google Scholar Pharmacy and Pharmacology, Volume 16, Issue Supplement_1, December 1964, Pages 9T–10T, https://doi.org/10.1111/j.2042-7158.1964.tb07531.x Published: 12 April 2011
Several recent studies have suggested that compounds known as endocrine-disrupting chemicals (EDCs) can promote obesity by serving ligands for nuclear receptors, including the peroxisome proliferator–activated receptor γ (PPARγ) and glucocorticoid (GR). Thiazolidinedione insulin sensitizers, which act PPARγ, also interact with regulate activity of mitochondrial pyruvate carrier (MPC). We evaluated whether several EDCs might affect MPC activity. Most did not acutely metabolism. However,...