A5100378630- CRISPR and Genetic Engineering
- PARP inhibition in cancer therapy
- Telomeres, Telomerase, and Senescence
- BRCA gene mutations in cancer
- RNA Interference and Gene Delivery
- RNA regulation and disease
- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- Advanced biosensing and bioanalysis techniques
- Renal and related cancers
- Asthma and respiratory diseases
- Virus-based gene therapy research
- Animal Genetics and Reproduction
- RNA and protein synthesis mechanisms
- Plant Gene Expression Analysis
- Salivary Gland Disorders and Functions
- Thermoregulation and physiological responses
- Bioinformatics and Genomic Networks
- Biological Research and Disease Studies
- Genetics, Aging, and Longevity in Model Organisms
- Single-cell and spatial transcriptomics
- Protein Degradation and Inhibitors
- Chromatin Remodeling and Cancer
- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
Northeastern University
2025
First Affiliated Hospital of Henan University
2025
Capital Medical University
2017-2025
Peking University
2016-2024
Stanford University
2015-2024
Texas Tech University
1999-2024
Chinese PLA General Hospital
2024
University Hospitals of Cleveland
2022-2024
Fudan University
2022-2024
Peking University Third Hospital
2016-2024
Telomere maintenance has been proposed as an essential prerequisite to human tumor development. The telomerase enzyme is itself a marker for cells, but the genetic alterations that activate during neoplastic transformation have remained mystery. Here, we show Myc induces in both normal mammary epithelial cells (HMECs) and diploid fibroblasts. increases expression of hEST2 (hTRT/TP2), limiting subunit telomerase, can extend life span HMECs. ability may contribute its promote formation.
An unbiased screen for genes that can immortalize mouse embryonic fibroblasts identified the glycolytic enzyme phosphoglycerate mutase (PGM). A 2-fold increase in PGM activity enhances flux, allows indefinite proliferation, and renders cells resistant to ras-induced arrest. Glucosephosphate isomerase, another enzyme, displays similar and, conversely, depletion of or glucosephosphate isomerase with short interfering RNA triggers premature senescence. Immortalized stem display higher flux more...
Pancreatic ductal adenocarcinoma (PDAC) is a genomically diverse, prevalent, and almost invariably fatal malignancy. Although conventional genetically engineered mouse models of human PDAC have been instrumental in understanding pancreatic cancer development, these are much too labor-intensive, expensive, slow to perform the extensive molecular analyses needed adequately understand this disease. Here we demonstrate that retrograde injection either adenoviral-Cre or lentiviral-Cre vectors...
Abstract Human induced pluripotent stem cells (iPSCs) and genome editing provide a precise way to generate gene-corrected for disease modeling cell therapies. iPSCs generated from sickle (SCD) patients have homozygous missense point mutation in the HBB gene encoding adult β-globin proteins, are used as model system improve strategies of human therapy. We demonstrate that CRISPR/Cas9 designer nuclease is much more efficient stimulating targeting endogenous locus near SCD than zinc finger...
// Lin Li 1, * , Yanwei Sha 2, Xi Wang 3 Ping 2 Jing 4 Kehkooi Kee 1 Binbin Center for Stem Cell Biology and Regenerative Medicine, Department of Basic Medical Sciences, School Tsinghua University, Beijing, 100084, China Reproductive Medicine Center, Xiamen Maternal Child Health Care Hospital, Xiamen, 361005, Fujian Province, Genetics, National Research Institute Family Planning, Haidian, 100081, Genetics Developmental Biology, Capital 100069, These authors have contributed equally to this...
Purpose.: The purpose of this study was to establish a fully validated, high-throughput next-generation sequencing (NGS) approach for comprehensive, cost-effective, clinical molecular diagnosis retinitis pigmentosa (RP). Methods.: Target sequences panel 66 genes known cause all nonsyndromic and few syndromic forms RP were enriched by using custom-designed probe hybridization. A total 939 coding exons 20 bp their flanking intron regions with 202,800 target captured, followed massively...
Histone acetylation plays an important role in chromatin remodeling and gene expression. The molecular mechanisms involved cell-specific expression of endothelial nitric-oxide synthase (eNOS) are not fully understood. In this study we investigated whether histone deacetylation was repression eNOS non-endothelial cells. Induction by deacetylase (HDAC) inhibitors trichostatin A (TSA) sodium butyrate observed all four different types cells examined. Chromatin immunoprecipitation assays showed...
The monitoring of genetically modified organisms (GMOs) is a primary step GMO regulation. However, there presently lack effective and high-throughput methodologies for specifically sensitively most the commercialized GMOs. Herein, we developed multiplex amplification on chip with readout an oligo microarray (MACRO) system convenient monitoring. This composed microchip multiple amplicons, containing total 91 targets (18 universal elements, 20 exogenous genes, 45 events, 8 endogenous reference...
The repression of telomerase activity during cellular differentiation promotes replicative aging and functions as a physiological barrier for tumorigenesis in long-lived mammals, including humans. However, the underlying mechanisms remain largely unclear. Here we describe how miR-615-3p represses hTERT expression. mir-615-3p is located an intron HOXC5 gene, member highly conserved homeobox family transcription factors controlling embryogenesis development. Unexpectedly, found that HoxC5 also...
ABSTRACT Somatic genome editing in mouse models has increased our understanding of the vivo effects genetic alterations areas ranging from neuroscience to cancer biology and beyond. However, existing are limited their ability create multiple targeted edits. Thus, complex interactions that underlie development, homeostasis, disease remains incomplete. Cas12a is an RNA-guided endonuclease with unique attributes enable simple targeting genes crRNA arrays containing tandem guides. To accelerate...
Flavonoids are specialized compounds widely distributed and with diverse functions throughout the plant kingdom several benefits for human health. In particular, flavonols, synthesized by flavonol synthase (FLS), protect plants against UV-B radiation essential male fertility in maize other plants. We have recently characterized a inducible ZmFLS1, corresponding to first be described monocot Interestingly, new assembly of B73 genome revealed presence second putative FLS gene (ZmFLS2), very...
Genetic variants are known causes of male infertility and oligoasthenoteratozoospermia (OAT), as shown by knockout mouse models patients with infertility. However, most OAT cases lack a definitive genetic diagnosis. Peripheral blood semen samples were collected from patient OAT. Semen analysis, Papanicolaou staining, transmission electron microscopy, whole-exome sequencing (WES), Sanger sequencing, in silico analyses, such conservative analysis conformational used to investigate the revealed...
The tissue optical clearing (TOC) technique could significantly improve the biomedical imaging depth, but most current investigations are limited to in vitro studies. For vivo applications, TOC method must provide a rapid treatment process, sufficient transparency, and safety for animals, which makes it more difficult. Recently developed innovative methods use show great potential enhancing contrast resolution of laser speckle (LSCI) blood flow monitoring. This paper gives an overview recent...