A5049957705- CRISPR and Genetic Engineering
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- Chromosomal and Genetic Variations
Columbia University
2022-2024
Insertion sequences (IS) are compact and pervasive transposable elements found in bacteria, which encode only the genes necessary for their mobilization maintenance. IS 200 /IS 605 undergo 'peel-and-paste' transposition catalyzed by a TnpA transposase, but intriguingly, they also diverse, TnpB- IscB-family proteins that evolutionarily related to CRISPR-associated effectors Cas12 Cas9, respectively. Recent studies demonstrated TnpB-family enzymes function as RNA-guided DNA endonucleases,...
Abstract Transposon-encoded tnpB genes encode RNA-guided DNA nucleases that promote their own selfish spread through targeted cleavage and homologous recombination 1–4 . This widespread gene family was repeatedly domesticated over evolutionary timescales, leading to the emergence of diverse CRISPR-associated including Cas9 Cas12 5,6 We set out test hypothesis TnpB may have also been repurposed for novel, unexpected functions other than CRIS-PR-Cas. Here, using phylogenetics, structural...