A5059162990- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Malaria Research and Control
- Bacterial Genetics and Biotechnology
- Ion channel regulation and function
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Parasites and Host Interactions
- Signaling Pathways in Disease
- Drug Transport and Resistance Mechanisms
- Trypanosoma species research and implications
- HIV Research and Treatment
- Animal Virus Infections Studies
- Invertebrate Immune Response Mechanisms
Columbia University
2023-2024
National Institute of Allergy and Infectious Diseases
2020-2022
National Institutes of Health
2020-2022
Medicines for Malaria Venture
2022
Microbiotix (United States)
2022
Abstract Accurate biomolecular structure prediction enables the of mutational effects, speculation function based on predicted structural homology, analysis ligand binding modes, experimental model building and many other applications. Such algorithms to predict essential functional features remain out reach for biomolecular. Here, we report quantitative qualitative evaluation nucleic acid structures CASP16 blind challenge by 12 groups who provided targets. Blind predictions accurately...
Malaria parasites activate a broad-selectivity ion channel on their host erythrocyte membrane to obtain essential nutrients from the bloodstream. This conserved channel, known as plasmodial surface anion (PSAC), has been linked parasite clag3 genes in P. falciparum, but epigenetic switching between two copies of this gene hinders clear understanding how encoded protein determines PSAC activity. Here, we used linkage analysis falciparum cross where one parent carries single overcome effects...
Insertion sequences (IS) are compact and pervasive transposable elements found in bacteria, which encode only the genes necessary for their mobilization maintenance. IS 200 /IS 605 undergo 'peel-and-paste' transposition catalyzed by a TnpA transposase, but intriguingly, they also diverse, TnpB- IscB-family proteins that evolutionarily related to CRISPR-associated effectors Cas12 Cas9, respectively. Recent studies demonstrated TnpB-family enzymes function as RNA-guided DNA endonucleases,...
Abstract Exoribonuclease-resistant RNAs (xrRNAs) are viral RNA structures that block degradation by cellular 5′-3′ exoribonucleases to produce subgenomic during infection. Initially discovered in flaviviruses, xrRNAs have since been identified wide range of viruses, including those infect plants. High sequence variability among raises questions about the shared molecular features characterize this functional class. Here, we present first structure a plant-virus xrRNA its active...
Human and animal malaria parasites increase their host erythrocyte permeability to a broad range of solutes as mediated by parasite-associated ion channels. Molecular pharmacological studies have implicated an essential role in parasite nutrient acquisition, but inhibitors suitable for development antimalarial drugs are missing. Here, we generated potent specific drug lead using <i>Plasmodium falciparum</i>, virulent human pathogen, derivatives MBX-2366, nanomolar affinity pyridazinone...