A5063777913- Hematopoietic Stem Cell Transplantation
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Organ Transplantation Techniques and Outcomes
- Organ and Tissue Transplantation Research
- TGF-β signaling in diseases
- Synthesis and biological activity
- T-cell and B-cell Immunology
- Carcinogens and Genotoxicity Assessment
- Advanced biosensing and bioanalysis techniques
- Click Chemistry and Applications
- Forensic Toxicology and Drug Analysis
- Cell Adhesion Molecules Research
- Acute Myeloid Leukemia Research
- Biosensors and Analytical Detection
- Blood transfusion and management
- Chronic Lymphocytic Leukemia Research
- Mycotoxins in Agriculture and Food
- Liver physiology and pathology
- Mast cells and histamine
- Molecular Biology Techniques and Applications
- Reproductive System and Pregnancy
- Blood donation and transfusion practices
- Chronic Myeloid Leukemia Treatments
- Transplantation: Methods and Outcomes
Moroccan Foundation for Advanced Science, Innovation and Research
2014-2024
KU Leuven
1997-2003
University of California, San Diego
2002-2003
Fund for Scientific Research
1999-2001
American Society of Transplantation
1999
Université Libre de Bruxelles
1998
In the present study, a series of thiazolidine-2,4-diones derivatives (3a–3e) and (4a–4e) were synthesized characterized by 1H NMR, 13C NMR ESI-MS spectrometry. All compounds screened for their α-glucosidase α-amylase inhibitory activities. vitro biological investigations revealed that most active against with IC50 values in range 43.85 ± 1.06 to 380.10 1.02 µM, 18.19 0.11 208.10 1.80 µM. Some tested found be more potent inhibitors than clinical drug Acarbose (IC50glucosidase = 97.12 0.35 µM...
Tuberculosis (TB) is a major global health problem and remains the leading cause of morbidity mortality in developing countries. Routinely used TB diagnostic methods, most endemic areas, are time-consuming, often less-sensitive, expensive inaccessible to patients. Therefore, there an urgent need for development early, easy use effective diagnosis tools TB, which can be effectively integrated into resource limited settings, anticipate early treatment limit further spread disease. Over last...
In this work, three isoxazoline-thiazolidine-2,4-dione derivatives were synthesized and characterized by FT-IR, 1H-NMR, 13C-NMR ESI-MS spectrometry. All compounds have been investigated for their α-amylase α-glucosidase inhibitory activities. vitro enzymatic evaluation revealed that all potent against with IC50 values varied from 40.67 ± 1.81 to 92.54 0.43 µM, in the range of 07.01 0.02 75.10 1.06 µM. One tested found be more inhibitor compared other standard drug Acarbose (IC50 glucosidase=...
Background. We have previously shown that allogeneic bone marrow (BM) chimeras preconditioned with total lymphoid irradiation and low-dose body (TLI/TBI) develop a stronger graft-versus-leukemia (GVL) effect than high-dose only (TBI). Here, we report on the possible role of cytokines in mechanism underlying this GVL effect. Methods. Splenic mRNA levels interleukin (IL)-1, IL-2, IL-4, IL-6, IL-7, IL-10, IL-12, IL-15, interferon-γ, tumor necrosis factor-α, transforming growth factor-β (TGF-β),...
The aim of the present study is to evaluate level contamination breast milk (BM) by ochratoxin A, among Moroccan lactating mothers in city Rabat, and identify associated factors exposure, also estimate degree exposure breastfeed infant. analysis A (OTA) was accomplished ELISA method on 82 colostrum samples. OTA detectable (>0.08 ng/mL) 55% samples with a maximum concentration 10.04 ng/mL, levels exceeded 0.5 ng /mL 50 % In addition, several dietary habits affect significantly analyzed...
Germline mutations in the BRCA1 gene are known predictive markers for development of hereditary breast cancer. Nevertheless, no comprehensive study has been performed targeting presence and relevance Moroccan cancer patients. We here present an analysis regions (exon 2 exon 11a/b) 50 female patients with early disease onset (≤ 40 years) or familial backgrounds. Results showed that mutation was either 11a any analysed. However, 11b, a generated by nucleotide exchange detected 8% patients,...
Background. Previous studies have demonstrated that delayed donor leukocyte infusion (DLI) can increase graft-versus-leukemia (GVL) without increasing graft-versus-host-disease (GVHD) in MHC mismatched bone marrow (BM) chimeras. In our report, the immune status of minor antigen BM chimeras given DLI was studied. Particularilly role ASGM1 positive or T cells effect investigated. Methods. AKR mice (H2k, Mls1a, Thy1.1) received TBI (9,5 Gy) and cell-depleted (TCD) C3H Mls2a, Thy1.2) alone (BM...
Background: Trastuzumab (Herceptin®) is currently the main treatment option for breast cancer patients that overexpress human epidermal growth factor receptor 2 (HER2). This antibody binds specifically to HER2, blocks cell growth, and promotes effective death. In present study, we sought develop a robust efficient process development of stable Chinese hamster ovary (CHO) line with high trastuzumab expression production. Methods: We adapted combines transposon system-based vector...
Rapid diagnosis of drug resistance in tuberculosis (TB) is pivotal for the timely initiation effective antibiotic treatment to prevent spread drug-resistant strains. The development low-cost, rapid and robust methods TB detection highly desirable resource-limited settings.We report use an house plasmid-based quantitative polymerase chain reaction-high-resolution melting (qPCR-HRM) analysis mutations related rifampicin-resistant Mycobacterium (MTB) clinical isolates from Moroccan patients....
Although in utero transplantation (IUT) has resulted donor-specific tolerance to postnatal solid organ transplantation, the mechanisms of this remain poorly understood. Our recent findings demonstrate that under specific conditions prenatal injection allogeneic cells may lead allosensitization instead tolerance. These laboratory observations were supported by clinical as well, and therefore suggested that, depending on or immunity develop. The present study explored role CD4 cells,...
Prostate cancer (PCa) remains one of the most widespread and perplexing all human malignancies. Assessment gene expression is thought to have an important impact on diagnosis, prognosis therapeutic decisions. In this context, we explored combined PCa related target genes AMACR PCA3 in 126 formalin fixed paraffin embedded prostate tissues (FFPE) from Moroccan patients, using quantitative real time reverse transcription-PCR (RT-qPCR). This quantification required data normalization...
Background. It has previously been demonstrated that adding T cell-depleted (TCD) host bone marrow (BM) to an MHC-mismatched BM inoculum allows for induction of long-term stable chimeras without graft-versus-host disease (GVHD) even when non-TCD allogeneic was used. Aims. The present study undertaken investigate immune tolerance mechanisms in minor antigen-mismatched host-type added the inoculum. Methods. C3H (H2k, Thy 1.2, Mls 2a) recipients were conditioned with 9.5 gray (Gy) total body...
Background. We have previously demonstrated that in a concordant hamster-to-nude rat cardiac transplant model, T-independent specific B-lymphocyte and natural killer (NK)-cell tolerance could be induced, leading to long-term xenograft (Xg) survival. Here, we investigated whether the same achieved clinically more relevant semi-discordant model involving hamster hearts transplanted into presensitized, nude rats. Methods. Sensitized, rats with high titers of anti-hamster immunoglobulin (Ig)M...
Molecular diagnosis of myeloid leukemia using qPCR who had received imatinib treatment (N = 20) were analyzed by realtime PCR, conventional RT-PCR, and FISH.Half the samples 10) positive for BCR-ABL gene expression, while other half negative according to PCR.Interestingly, 5 10 shown be PCR showed expression RT-qPCR.The RT-qPCR results confirmed FISH, which revealed a high concordance (100%) rate.We found that real-time is more reliable should used in Moroccan biomedical analysis...