A5024707203- Retinal Development and Disorders
- CRISPR and Genetic Engineering
- Neuroscience and Neural Engineering
- Retinal Diseases and Treatments
- Photoreceptor and optogenetics research
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Retinal and Optic Conditions
- Retinal and Macular Surgery
- Corneal Surgery and Treatments
- melanin and skin pigmentation
- CAR-T cell therapy research
- Ocular Surface and Contact Lens
- 3D Printing in Biomedical Research
- Corneal surgery and disorders
- Connexins and lens biology
- Glaucoma and retinal disorders
- Biochemical Acid Research Studies
- EEG and Brain-Computer Interfaces
- RNA regulation and disease
- Animal Genetics and Reproduction
- Supramolecular Self-Assembly in Materials
- Metabolism and Genetic Disorders
- Biomedical Ethics and Regulation
- Viral Infectious Diseases and Gene Expression in Insects
RWTH Aachen University
2015-2025
Weatherford College
2022
Leoni (Germany)
2022
Universitätsklinikum Aachen
2014-2019
Universitätsklinik für Augenheilkunde
2019
Ophthalmology Clinic
2019
Center for Clinical Research (United States)
2011
Forschungszentrum Jülich
2009
University of Stuttgart
2007
Abstract Background Retinal implants have been developed to treat blindness causing retinal degenerations such as Retinitis pigmentosa (RP). The stimulators are covering only a small portion of the retina usually in its center. To restore not central vision but also useful visual field need cover larger area retina. However, large much more difficult implant into an eye. Some basic questions concerning this challenge should be answered series experiments. Methods Large were fabricated...
Background . To evaluate the correlation between ERG, OCT, and microscopic findings in rd10 mouse. Methods C57BL/6J wild type mice were compared at age of 2, 3, 5, 7, 9, 12, 24, 48 weeks (each group<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mi>n</mml:mi><mml:mo>=</mml:mo><mml:mn>3</mml:mn></mml:math>) using full-field electroretinography (ERG), spectral domain Optical Coherence Tomography (sd-OCT), fluorescein angiography (FA), Hematoxylin & Eosin histology...
Subretinal transplantation of retinal (RPE) or iris (IPE) pigment epithelial cells has been advocated as a treatment for degeneration. However, to our knowledge, in patients with age-related macular degeneration no significant beneficial effects on vision have shown. Since the transplanted did not appear maintain healthy avascular and neuroprotective environment, we postulate that it will be necessary transplant express elevated levels anti-angiogenic activities. In study, provide protocol...
Purpose.: To characterize the effects of intravitreal injections N-methyl-N-nitrosourea (MNU) in comparison to its systemic application as a measure inducing unilateral photoreceptor degeneration. Methods.: Eight-week-old male C57BL/6J mice received either intraperitoneal (three animals) or (24 MNU different concentrations and were observed over period 2 weeks using full-field electroretinography (ERG), spectral-domain optical coherence tomography (SD-OCT), immunohistochemistry. Results.:...
The restoration of vision in blind patients suffering from degenerative retinal diseases like retinitis pigmentosa may be obtained by local electrical stimulation with implants. In this study, a very large electrode array for (VLARS) was introduced and tested regarding its safety implantation biocompatibility. Further, the array's capabilities were an acute setting.The polyimide-based implants have diameter 12 mm, cover approximately 110 mm2 surface carrying 250 iridium oxide coated gold...
In the treatment of blindness causing retinal dystrophies, that is, retinitis pigmentosa (RP), implants showed promising results. Recently, larger devices restoring a greater visual field were introduced. With size, implantation surgery became more difficult. this study, novel device was developed, fabricated, and tested in surgeries. The goal to demonstrate reproducible, safe, and, comparison, superior method. 3D-Printed Large-Array Port-System (3D-PLAPS) designed using computer-aided...
Repetitive intravitreal injections of bevacizumab are a successful treatment option for exudative age-related macular degeneration (AMD). The aim this study was to evaluate the toxicity in adult mammalian neurosensory retina culture.Adult porcine retinas were cultured adjoined retinal pigment epithelium-choroid layer (retina-RPE-choroid complex) static culture 3 days, whereas neural alone perfusion chamber days. Bevacizumab added and medium at three concentrations (0.25 mg/mL [n = 6], 0.5...
Neovascular age-related macular degeneration (nvAMD) is characterized by choroidal blood vessels growing into the subretinal space, leading to retinal pigment epithelial (RPE) cell and vision loss. Vessel growth results from an imbalance of pro-angiogenic (e.g., vascular endothelial factor [VEGF]) anti-angiogenic factors epithelium-derived [PEDF]). Current treatment using intravitreal injections anti-VEGF antibodies improves in about 30% patients but may be accompanied side effects...
Drug delivery systems (DDS) are able to deliver, over long periods of time, therapeutic concentrations drugs requiring frequent administration. Two classes DDS available, biodegradable and non-biodegradable. The larger non-biodegradable implants ensure long-term delivery, but require surgical interventions. Biodegradable biomaterials smaller, injectable implants, degrade hydrolytically release in non-zero order kinetics, which is inefficient for sustained drug release. poly(ester amides)...
The retinal degeneration 10 (rd10) mouse model is widely used to study retinitis pigmentosa (RP) pathomechanisms. It offers a rather unique opportunity trans-neuronal because the cell populations in question are separated anatomically and mutated Pde6b gene selectively expressed rod photoreceptors. We hypothesized that RNA binding protein (RBP) aggregation abnormal autophagy might serve as early pathogenic events, damaging non-photoreceptor types not primarily targeted by defect. combination...
Pigment epithelium-derived factor (PEDF) is a potent multifunctional protein that inhibits angiogenesis and has neurogenic neuroprotective properties. Since the wet form of age-related macular degeneration characterized by choroidal neovascularization (CNV), PEDF would be an ideal candidate to inhibit CNV support retinal pigment epithelial (RPE) cells. However, its short half-life precluded clinical use. To deliver subretinal space, we transfected RPE cells with gene using Sleeping Beauty...
While anterior chamber air bubbles aid attachment during posterior lamellar surgery only for few days, these periods can be prolonged with gases in non-expanding concentrations. To test the effects of different gas compositions on immortalized human corneal endothelial cells (HCEC-12), we utilized Transwell inserts semipermeable membranes as an artificial model.Human (HCEC-12) were cultured 24 hr, then flipped, burdened and sunk titanium rings medium (M1), well filled 2 ml (A), 20% sulphur...
Abstract The isolation and propagation of primary human corneal stromal keratocytes (CSK) are crucial for cellular research tissue engineering. However, this delicate cell type easily transforms into fibroblasts (SF) scar inducing myofibroblasts (Myo‐SF). Current protocols mainly rely on xenogeneic fetal bovine serum (FBS). Human platelet lysate (hPL) could be a viable, potentially autologous, alternative. We found high survival with both supplements in CSK SF. Cell numbers Ki67+ ratios...
Introduction: Visual prostheses, i.e. epiretinal stimulating arrays, are a promising therapy in treating retinal dystrophies and degenerations. In the wake of new generation devices, an innovative method for fixation stimulator arrays is required. We present development tailor-made bioadhesive peptides (peptesives) fixating omitting use traumatic tacks. Materials methods: Binding motifs on array (poly[chloro-p-xylylene] (Parylene C)) extracellular matrix surface (collagens I IV, laminin,...
The anti-angiogenic and neurogenic pigment epithelium-derived factor (PEDF) demonstrated a potency to control choroidal neovascularization in age-related macular degeneration (AMD) patients. goal of the present study was development an efficient safe technique integrate, ex vivo, PEDF gene into retinal epithelial (RPE) cells for later transplantation subretinal space AMD patients allow continuous secretion vicinity affected macula. Because successful therapy approaches require delivery...