A5043346003- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- Chemistry and Chemical Engineering
- Inorganic and Organometallic Chemistry
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Click Chemistry and Applications
- Supramolecular Chemistry and Complexes
- Carbohydrate Chemistry and Synthesis
- Nanomaterials for catalytic reactions
- RNA modifications and cancer
- Pharmaceutical and Antibiotic Environmental Impacts
Novo Nordisk (Denmark)
2024-2025
Janssen (Belgium)
2021-2024
Institut des Biomolécules Max Mousseron
2018-2022
Centre National de la Recherche Scientifique
2022
University of Southern Denmark
2019-2021
Université de Montpellier
2018
École Nationale Supérieure de Chimie de Montpellier
2018
University of Turku
2013-2015
5′-O-(2-Methoxyisopropyl) (MIP)-protected 2′-deoxynucleosides as chiral P(V)-building blocks, based on the limonene-derived oxathiaphospholane sulfide, were synthesized and used for assembly of di-, tri-, tetranucleotide phosphorothioates a tetrapodal pentaerythritol-derived soluble support. The synthesis cycle consisted two reactions precipitations: (1) coupling under basic conditions, followed by neutralization precipitation (2) an acid catalyzed 5′-O-deacetalization, precipitation. simple...
Abstract 5′‐ O ‐(4,4′‐Dimethoxytrityl)thymidine was attached to a pentaerythrityl‐derived core, and the resulting tetravalent nucleoside cluster next dendritic generations served as soluble support for synthesis of short oligo‐2′‐deoxyribonucleotides in solution. Couplings using small excess (1.5 equiv. per 5′‐OH group) standard phosphoramidite building blocks proved efficient, products could be purified by quantitative precipitation from methanol. Ammonolysis released nearly homogeneous...
An effective method for the synthesis of short oligoribonucleotides in solution has been elaborated. Novel 2'-O-(2-cyanoethyl)-5'-O-(1-methoxy-1-methylethyl) protected ribonucleoside 3'-phosphoramidites have prepared and their usefulness as building blocks RNA on a soluble support demonstrated. As proof concept, pentameric oligoribonucleotide, 3'-UUGCA-5', precipitative tetrapodal tetrakis(4-azidomethylphenyl)pentaerythritol support. The 3'-terminal nucleoside was coupled to...
ADVERTISEMENT RETURN TO ISSUEPREVGreen Chemistry High...Green HighlightsNEXTGreen Articles of Interest to the Pharmaceutical IndustryMelissa A. AshleyMelissa AshleyGenentech Inc., 1 DNA Way, South San Francisco, California 94080, United StatesMore by Melissa Ashley, Miles H. AuklandMiles AuklandChemical Development, Technology & Operations, AstraZeneca, Macclesfield SK10 2NA, U.K.More Aukland, Marian C. Bryan*Marian BryanJanssen R&D, 1400 McKean Road, Spring House, Pennsylvania 19002,...
The synthesis of a 5′-boronoribonucleotidic phosphoramidite building block has been achieved and incorporated at the 5′ extremities RNA sequences for templated assembly shortmers.
The preparation of a disulfide-tethered precipitative soluble support and its use for solution-phase synthesis trimeric oligodeoxyribonucleotide 3´-(2-chlorophenylphosphate) building blocks is described. To obtain the blocks, N-acyl protected 2´-deoxy-5´-O-(4,4´-dimethoxytrityl)ribonucleosides were phosphorylated with bis(benzotriazol-1-yl) 2-chlorophenyl phosphate. "outdated" phosphotriester strategy, based on coupling P(V) in conjunction quantitative precipitation MeOH applied. Subsequent...
Solubility of the growing oligonucleotide-soluble support constructs in liquid phase oligonucleotide synthesis (LPOS) is a critical parameter, which affects coupling efficiency, purity, and recovery oligonucleotides during chain elongation. In present study, have been assembled on 4-oxoheptanedioic acid (OHDA) linker-derived tetrapodal soluble using 5'-
ADVERTISEMENT RETURN TO ISSUEPREVGreen Chemistry High...Green HighlightsNEXTGreen Articles of Interest to the Pharmaceutical IndustryMarian C. Bryan*Marian BryanJanssen R&D, 1400 McKean Road, Spring House, Pennsylvania 19002, United States*Email: [email protected]More by Marian Bryan, Charlotte DaltonCharlotte DaltonCatSci Ltd, CBTC2, Capital Business Park, Cardiff, South Glamorgan CF3 2PX, U.K.More Dalton, Alba Díaz-RodríguezAlba Díaz-RodríguezSynthetic Biochemistry, GSK, Gunnels Wood...
ADVERTISEMENT RETURN TO ISSUEPREVGreen Chemistry High...Green HighlightsNEXTGreen Articles of Interest to the Pharmaceutical IndustryMarian C. Bryan*Marian BryanJanssen R&D, 1400 McKean Road, Spring House, Pennsylvania 19002, United States*Email: [email protected]More by Marian Bryan, Charlotte DaltonCharlotte DaltonCatSci Ltd, CBTC2, Capital Business Park, Cardiff, South Glamorgan CF3 2PX, U.K.More Dalton, Alba Díaz-RodríguezAlba Díaz-RodríguezSynthetic Biochemistry, GSK, Gunnels Wood...
ADVERTISEMENT RETURN TO ISSUEPREVGreen Chemistry High...Green HighlightsNEXTGreen Articles of Interest to the Pharmaceutical IndustryMelissa A. AshleyMelissa AshleyGenentech Inc., 1 DNA Way, South San Francisco, California 94080, United StatesMore by Melissa Ashley, Miles H. AuklandMiles AuklandChemical Development, Technology & Operations, AstraZeneca, Macclesfield SK10 2NA, U.K.More Aukland, Marian C. Bryan*Marian BryanJanssen R&D, 1400 McKean Road, Spring House, Pennsylvania 19002,...
The construction of boronate ester-linked macrocyclic di- and trinucleotides by the head-to-tail assembly a ribonucleotide precursor containing boronic acid is reported.
ADVERTISEMENT RETURN TO ISSUEPREVGreen Chemistry High...Green HighlightsNEXTGreen Articles of Interest to the Pharmaceutical IndustryMarian C. Bryan*Marian BryanJohnson & Johnson Limited, 1400 McKean Road, Spring House, Pennsylvania 19002, United States*Email: [email protected]More by Marian Bryan, Charlotte DaltonCharlotte DaltonCatSci Ltd, CBTC2, Capital Business Park, Cardiff, South Glamorgan CF3 2PX, U.K.More Dalton, Louis J. DiorazioLouis DiorazioChemical Development, Technology...
Azido nucleosides couple with phosphoramidites via an initial iminophosphorane, which eliminates acrylonitrile to generate the coupled dimer P(V) product. The vulnerable phosphite triester intermediate is bypassed entirely, making methodology very suitable solution-phase synthesis. This new coupling protocol requires no protection of 5'-OH function and provides a method installing internucleosidic phosphorodiamidate bonds near quantitative yields.
ADVERTISEMENT RETURN TO ISSUEPREVGreen Chemistry High...Green HighlightsNEXTGreen Articles of Interest to the Pharmaceutical IndustryMarian C. Bryan*Marian BryanJanssen R&D, 1400 McKean Road, Spring House, Pennsylvania 19002, United States*Email: [email protected]More by Marian Bryan, Charlotte DaltonCharlotte DaltonCatSci Ltd, CBTC2, Capital Business Park, Cardiff, South Glamorgan CF3 2PX, U.K.More Dalton, Jaika DoerflerJaika DoerflerDrug Substance Technologies, Amgen Inc., One Center...