A5101669007- Salmonella and Campylobacter epidemiology
- Animal Nutrition and Physiology
- Essential Oils and Antimicrobial Activity
- Aquaculture disease management and microbiota
- Listeria monocytogenes in Food Safety
- Viral gastroenteritis research and epidemiology
- Escherichia coli research studies
- Probiotics and Fermented Foods
- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- Food Safety and Hygiene
- Gut microbiota and health
- Antimicrobial Peptides and Activities
- Livestock and Poultry Management
- Microbial infections and disease research
- Nanocomposite Films for Food Packaging
- Coccidia and coccidiosis research
- S100 Proteins and Annexins
- Bacteriophages and microbial interactions
- Natural Products and Biological Research
- Mosquito-borne diseases and control
- Microbial Metabolism and Applications
- Microbial Inactivation Methods
- Chemical and Physical Properties in Aqueous Solutions
- Fatty Acid Research and Health
Roslin Institute
2025
University of Edinburgh
2025
Agri Food and Biosciences Institute
2011-2025
Hospital of the University of Pennsylvania
1993
Abstract Background Maintaining gut health is a persistent and unresolved challenge in the poultry industry. Given critical role of chicken performance welfare, there pressing need to identify effective intervention (GHI) strategies ensure optimal outcomes farming. In this study, across three broiler production cycles, we compared metagenomes broilers provided with ionophores (as control group) against birds subjected five different GHI combinations involving vaccination, probiotics,...
Chickens are a key food source for humans yet their microbiome contains bacteria that can be pathogenic to humans, and indeed potentially chickens themselves. Campylobacter is present within the chicken gut leading cause of bacterial foodborne gastroenteritis worldwide. Infection lead secondary sequelae such as Guillain-Barré syndrome stunted growth in children from low-resource areas. Despite global health impact economic burden Campylobacter, how when appears remains unclear. The lack day...
The factors affecting host-pathogen ecology in terms of the microbiome remain poorly studied. Chickens are a key source protein with gut health heavily dependent on complex which has roles nutrient assimilation and vitamin amino acid biosynthesis. chicken may be influenced by extrinsic production system parameters such as Placement Birds/m2 (stocking density), feed type additives. Such parameters, addition to on-farm biosecurity influence performance also pathogenic bacterial numbers...
The increased resistance of campylobacters to antibiotics required the identification and isolation novel antimicrobials able inhibit its virulence, cause less or no display host toxicity. Acquiring all this knowledge was only possible through a better understanding their antibacterial potency biological mechanisms involved attenuating bacterial virulence factors. This review describes most recent developments in area by looking at new antimicrobial interventions aiming combat transmission...
When isolating Campylobacter spp. from retail raw chicken using BS EN ISO 10272-1:2006, contaminants frequently cause overgrowth on mCCDA plates. Therefore, these organisms proliferate in the enrichment medium, Bolton broth, indicating a lack of selectivity this medium. This study sought to characterize contaminant flora and devise modified broth inhibit their growth.Contaminants (n=30) separate samples were identified antibiotic resistances determined. Most (93%) extended spectrum...
The current trend in reducing the antibiotic usage animal production imposes urgency identification of novel biocides. essential oil carvacrol, for example, changes morphology cell and acts against a variety targets within bacterial membranes cytoplasm, our vitro results show that it reduces adhesion invasion chicken intestinal primary cells also biofilm formation. A trial was conducted to evaluate effects dietary supplementation carvacrol at four concentrations (0, 120, 200, 300 mg/kg diet)...
Human campylobacteriosis is considered one of the most common foodborne diseases worldwide with poultry identified as main source infection accounting for 50-80% human cases. Highly virulent Campylobacter spp., positive Type VI secretion system (T6SS), which have an increased ability to adhere and invade host gastrointestinal epithelium are highly prevalent in poultry. Multidrug resistant strains bacteria rapidly evolving therefore, new antimicrobials supplement animal feed that able control...
Recently the Type VI secretion system (T6SS), which can play a significant role in bacterial survival and pathogenesis, was reported Campylobacter spp., having hcp gene as key component.Campylobacteriosis is associated with consumption of infected chicken meat. Our study aimed to explore presence T6SS C. jejuni (n = 59) coli 57) isolates, from retail raw investigate their pathogenic potential. The used an indicator for presence.Using multiplex PCR we have identified significantly higher...
Abstract Eimeria tenella and bovis are complex parasites responsible for the condition of coccidiosis, that invade animal gastrointestinal intestinal mucosa causing severe diarrhoea, loss appetite or abortions, with devastating impacts on farming industry. The negative these parasitic infections enhanced by their role in promoting colonisation gut common foodborne pathogens. aim this study was to test anti-Eimeria efficacy maltodextrin, sodium chloride, citric acid, citrate, silica, malic...
In humans, Pseudomonas aeruginosa is well known as a prominent opportunistic pathogen associated with antimicrobial resistance (AMR), which presents major challenge to successful treatment. This also the case in animals, particularly companion dogs where P. common cause of otitis. Despite its clinical significance, little data are available on genomics and epidemiology dogs. To address this, we have genome-sequenced 34 canine otitis isolates from veterinary referral hospital analysed these...
Listeriosis is an important food-borne disease responsible for high rates of morbidity and mortality. L. monocytogenes has been the cause several foodborne outbreaks its ability to adapt survive in a wide range environmental conditions makes eradication difficult. Many strains are avirulent but have increase their virulence if exposed stresses. The aim this study was explain observed outbreak isolates by using phenotypic assays whole genome sequencing. Four from sweetcorn one isolate raw...
This study sought to determine the most effective protocol for detection of Campylobacter spp. in retail packs fresh, raw chicken based on ISO 10272-1:2006.Three sample preparation protocols were studied; two excision and one combining with a rinse remaining sample. Enrichment cultures incubated both closed bottles microaerobically, sub-cultured at 24 48 h. Packs (110) analysed only yielded no Subculturing enrichment broths h gave same prevalence as h, P > 0.4 but microaerobic incubation...
Geraniol and linalool have been found to be effective against foodborne microorganisms in vitro. However, due their hydrophobic nature, it is difficult achieve an even dispersion foods with high water content resulting dramatic loss of activity. The aim the study was fabricate geraniol or nanoemulsions investigate effect Escherichia coli K12, Listeria innocua Pseudomonas lundensis a meat simulation medium. agar diffusion assay revealed that both had potent antimicrobial activity all...
Ruminants are important reservoirs of E. coli O157:H7 and considered as the major source most foodborne outbreaks (e.g., 2017 outbreak in Germany, 2014 2016 United States, all linked to beef products). A promising strategy reduce O157 is using antimicrobials pathogen levels and/or virulence within animal gastrointestinal tract thus disease. The aim study was determine efficacy a commercial mixture natural against O157. minimum inhibitory concentration bactericidal antimicrobial were...
Reducing acute mortality in aquatic crustaceans using natural alternatives to antibiotics has become a necessity, firstly for its positive impact on the aquaculture industry and, secondly, because extensive use of may lead increased levels drug resistance pathogenic microorganisms. This study aimed investigate effect mixture antimicrobials vitro and vivo virulence abilities Type VI secretion system (T6SS)-positive Vibrio parahaemolyticus (A3 D4), strains known as having potentially harmful...
Abstract Background The classification of natural antimicrobials as potential antibiotic replacements is still hampered by the absence clear biological mechanisms behind their mode action. This study investigated underlying anti-bacterial effect a mixture (maltodextrin, citric acid, sodium citrate, malic citrus extract and olive extract) against Campylobacter jejuni RC039, Salmonella enterica SE 10/72 Clostridium perfringens ATCC® 13124 invasion Madin–Darby Canine Kidney cells (MDCK)....
Cryptosporidium is a major cause of diarrhea worldwide in both humans and farm animals with no completely effective treatment available at present. In this study, we assessed the inhibitory effect different concentrations Auranta 3001 (0.1, 0.5 1%), novel natural feed supplement, on C. hominis parvum invasion human ileocecal adenocarcinoma (HCT-8), bovine primary cells HCT-8, intestinal biopsies. The supplement production pro-inflammatory cytokines IL-8 INF-γ, anti-inflammatory cytokine...
Poultry farming is growing globally, particularly in developing countries, to meet the demands of populations for poultry meat and eggs. This likely lead an increase use antibiotics feed, thus contributing development spread antibiotic resistance which, poses a serious threat human animal health worldwide. One way reducing this reduce production by finding effective sustainable alternatives that can be used support productivity. Therefore, study evaluates incorporation three medicinal...
Anti-virulence strategies are an alternative approach to combat zoonotic bacterial pathogens. Although control measures in place against E. coli O157, it still remains a global health concern with cattle being the most important reservoir. Different feed constituents have varying effects on virulence of O157 present gut which then transferred meat processing surfaces and during slaughter. This study explored anti-virulence properties mixture natural plant extracts organic acids (citrus,...
Camelina (Camelina sativa L. Crantz) or false flax is an oil crop from the Brassicaceae family. has multiple uses, of which best known biofuel production. A motivation for this study was oil’s high content omega-3, omega-6 and omega-9 fatty acids, are to have antimicrobial effect. Campylobacter most common bacterial cause human foodborne gastroenteritis in world. We tested potential effect free acids (FFA) camelina against lactic acid bacteria (LAB) Campylobacter. The vitro results show that...
Maintaining gut health is a persistent and unresolved challenge in the poultry industry. Given critical role of chicken performance welfare, there pressing need to identify effective intervention (GHI) strategies ensure optimal outcomes farming. In this study, across three broiler production cycles, we compared metagenomes broilers provided with ionophores as control against birds subjected five different GHI combinations involving vaccination, probiotics, prebiotics, essential oils,...