- Polysaccharides and Plant Cell Walls
- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Microbial Metabolites in Food Biotechnology
- Legume Nitrogen Fixing Symbiosis
- Polysaccharides Composition and Applications
- Plant nutrient uptake and metabolism
- Hydrocarbon exploration and reservoir analysis
- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Atmospheric and Environmental Gas Dynamics
- Postharvest Quality and Shelf Life Management
- Advanced Cellulose Research Studies
- Plant Reproductive Biology
- Plant-Microbe Interactions and Immunity
- Petroleum Processing and Analysis
- Methane Hydrates and Related Phenomena
- Enzyme-mediated dye degradation
- Plant Molecular Biology Research
- Geochemistry and Geologic Mapping
- Analytical Chemistry and Chromatography
- Plant Pathogenic Bacteria Studies
- Plant Surface Properties and Treatments
- Proteoglycans and glycosaminoglycans research
- Enhanced Oil Recovery Techniques
Natural Resources Canada
2018-2022
Oklahoma State University
2010-2020
Geological Survey of Canada
2016-2018
Oklahoma State University Oklahoma City
2007-2008
City College of New York
2008
The Graduate Center, CUNY
2008
College of Staten Island
2008
City University of New York
2008
Institut des Sciences de la Terre d'Orléans
2004-2005
Institut des Sciences de la Terre
2004-2005
A revised system of abbreviated names is proposed for xyloglucan‐derived oligosaccharides. Each (1→4)‐linked β‐ d ‐glucosyl residue (and the reducing terminal ‐glucose moiety) backbone given a one‐letter code according to its substituents. The name oligosaccharide consists these letters listed in sequence from non‐reducing terminus backbone.
To facilitate analysis of plant cell wall polysaccharide structure and composition, we cloned 74 genes encoding polysaccharide-degrading enzymes from Aspergillus nidulans, fumigatus, Neurospora crassa expressed the as secreted proteins with C-terminal Myc 6x His tags. Most recombinant were active in enzyme assays, optima for pH temperature established. A subset was used to fragment polysaccharides irregular xylem 9 (irx9) mutant Arabidopsis. The revealed a decrease abundance xylan mutant,...
Plant roots secrete a complex polysaccharide mucilage that may provide significant source of carbon for microbes colonize the rhizosphere. High molecular weight was separated by high-pressure liquid chromatography gel filtration from low components pea root exudate. Purified generally similar in sugar and glycosidic linkage composition to cowpea, wheat, rice, maize, but appeared contain an unusually high amount material arabinogalactan protein. used as sole growth several rhizosphere...
Extensin, a major hydroxyproline (Hyp)-rich glycoprotein in walls of cultured cells dicotyledonous plants, is very difficult to solubilize. To learn about the nature insolubilization, we have tested ability variety selective hydrolytic methods, and combinations them, liberate extensin or fragments from suspension-culture cell walls. After complete deglycosylation cotton (Gossypium hirsutum L.) walls, trypsinization solubilized 80% Hyp. The sequences three abundant peptides were: (a)...
The extracellular polysaccharide produced by the bacterium Rhizobium japonicum has been implicated in recognition between symbionts which takes place association of R with soybean.The complete primary structure japonicwn strain 3Ilb 138 determined a combination conventional and unconventional methods.The polymer contains glucose, mannose, galacturonic acid molar ratio 2:1:1 varying proportion galactose 4-Omethylgalactose, sum these two being equivalent to amount mannose (Mort, A. J., Bauer,...
The chemical compositions of the capsular and extracellular polysaccharides two strains Rhizobium japonicum (311b 138 110) have been determined correlated as a function culture age with ability bacteria from which they were obtained to bind soybean seed lectin.Each contains approximately constant amounts mannosyl, glucosyl, galacturonosyl residues in molar ratio 1:2:1. In addition contain variable galactosyl 4-O-methyl residues. total galactose plus galactose, however, is equivalent amount...
Abstract Background Fungi are important players in the turnover of plant biomass because they produce a broad range degradative enzymes. Aspergillus nidulans, well-studied saprophyte and close homologue to industrially species such as A. niger oryzae , was selected for this study. Results nidulans grown on sorghum stover under solid-state culture conditions 1, 2, 3, 5, 7 14 days. Based analysis chitin content, grew be 4-5% total after 2 days then maintained steady state 4% next 12 A hyphal...
Plants possess an efficient nonself surveillance system triggering induced disease resistance mechanisms upon molecular recognition of microbial invaders. Successful pathogens have evolved strategies to evade or counteract these mechanisms, e.g., by the generation suppressors. Pectic fragments produced during host cell wall degradation can act as endogenous suppressors hypersensitive response in wheat leaves. We isolated and characterized ho-mogalacturonans from walls two cultivars...
The classification of actinomycetes in the genus Frankia Brunchorst has been based partly on morphology but primarily their ability to form N 2 -fixing nodules roots actinorhizal host plants. To identify nonsporulating and noninfective isolates, biochemical tests are needed. Sugars present whole-cell hydrolysates have proven useful taxonomy other genera actinomycetes. We, others, found a sugar all isolates examined thus far that is absent tested. After reduction with NaBD H 4 acetylation,...