Context Rumen microbiota plays an important role in the evaluation of roughages. Gum astragalus hay is a non-traditional roughage ruminant feeding, and it curious how affects rumen comparison with lucerne (LH). Aim This study compares vitro true digestibility fermentation parameters (LH) gum astralagus (Astragalus glycyphyllos, AG), as well metagenomic profile bacterial community sheepruminal fluid following 48 h incubation either forage type. Methods The was carried out Daisy incubator. A...

The aim of this study was to determine the changes in mRNA transcription levels HSP70 and IGF genes related stress immunity Oncorhynchus mykiss obtained from fish farms, phenotypic antimicrobial properties bacteria isolated compare results according different diseases. Accordingly, six each farm, a total 30 were sampled. Bacterial identification, inoculations carried out tissue samples taken kidney symptomatic surfaces TSA. Primary cultures after incubation periods at 21 37oC. Gram staining,...

This study aimed to determine the effect of rosemary (Rosmarinus officinalis) essential oil (REO) in lamb fattening diets on blood, rumen parameters, performance and meat quality. Thirty Norduz male lambs weaned at 4 months age with average body weight 22.0 ± 4.41 kg were used. Lambs divided into three groups: no (control, R0), 250 mg/kg DM (R250) 500 (R500) added basal ration, fed for 70 d. The daily feed intake R500 (1.63 kg) was lower than other groups (1.70 (p = .01). Rosemary did not...

Abstract In this study, it is aimed to investigate the effects of Moringa oleifera and Sorbus domestica plant extracts on bacterial disease agents Yersinia ruckeri in aquaculture. Morphological biochemical properties 2 different Y. isolates were determined. Then, Real-Time PCR analysis gene sequencing identified. Phytochemicals (M. S. domestica) antibiotics (Oxytetracycline (OX) Enrofloxacin (ENR)) used together antibiogram test compared effect status antibiotics. Also, phytochemicals growth...

Abstract Bee venom (BV) is a rich source of secondary metabolites from honeybees ( Apis mellifera L.). It contains variety bioactive ingredients including peptides, proteins, enzymes, and volatile metabolites. This study investigated the antibacterial effects bee obtained honey bees L.) against bacterial fish pathogens, such as Lactococcus garvieae (Lg1, Lg2, Lg3), Vibrio anguillarum (Va1, Va2, Va3), Yersinia ruckeri (Yr1, Yr2, Yr3), Aeromonas hydrophila Ah1, Ah2, Ah3) expression levels on...

HIGHLIGHTS Hydrogen-rich water can be applied as non-toxic compound for the treatment of bacterial infectious diseases fish without any residual effects on or water. IL-1β and IL-6 gene expression levels decreased with increase hydrogen in HRW could reduce prevent death zebrafish infected L. garvieae (G5).

Unsuitable environmental conditions and low host resistance in the aquatic environment are most important factors fish diseases. Bacterial diseases can be treated with different preparations. The aim of this study is to determine disease symptoms, expression levels TNF IL genes some biochemical parameter changes Archocentrus centrarchus by using herbal extract for treatment caused Lactococcus garvieae bacteria. Gene was performed Real-Time PCR enzyme activities were determined...

Staphylococcus aureus is a Gram-positive, round-shaped bacterium that member of the Firmicutes. It often positive for catalase and nitrate reduction. Pathogenic strains promote infections by producing virulence factors such as potent protein toxins, expression cell-surface binds inactivates antibodies. Sepsis-related to methicillin-resistant S. (MRSA) has significant morbidity high mortality rates (15-30%). The methicillin resistance coded with mecA gene, while sensitivity nuc they are...

Van Basin has a rich geographical structure in terms of water resources and endemic fish species. In this study, Ligula intestinalis parasite, which is the host Barbus ercisianus living Nemrut Crater Lake, was recorded for first time. The parasite described molecularly as well morphologically. context, primer set designed molecular identification 28S rRNA gene region L. intestinalis. Real-Time PCR results with primers gave positive all samples. were confirmed by single peak (87) HRM analysis.

Abstract Injuries are an undesirable situation in fish kept aquariums. The use of antibiotics wound healing is not preferred due to the development antibiotic resistance bacteria and for economic reasons. This study was conducted demonstrate efficacy Juniperus root extract experimentally induced incised wounds yellowtail acei. Wound closure time, morphology, malondialdehyde content, glutathione s-transferase activity, changes gene expression levels, non-dispersibility aqueous environment,...

Aras Nehri coğrafik ve ekolojik anlamda çok önemli bir yere sahiptir. Nehrindeki Streptomyces çeşitliliğinin tespit edilmesi, izolasyonu, teşhisi karakterizasyonunun, mikrobiyolojik moleküler biyoloji genetik alanlarında bilim dünyasına katkı sağlayacağı düşünülmektedir. türleri dünyası için sahiptir, çünkü bu bakteri türlerinin ürettiği başta antibiyotikler olmak üzere birçok madde bulunmaktadır. Nehrinin geçtiği Erzurum Horasan, Kars Karakurt Iğdır Tuzluca bölgelerinden alınan numuneler...

Abstract Myxobolus cerebralis , a myxosporean parasite, is the etiological agent of whirling disease in salmonids, which responsible for high mortality and significant economic losses rainbow trout ( Oncorhynchus mykiss ) populations. Тhis investigation was conducted to establish blood biochemical changes caused by M. fill gaps from insufficient studies on parameters. Furthermore, aimed identify negative effect some key physiological parameters monitor its effects animal’s metabolism. The...

Abstract Injuries are an undesirable situation in fish kept aquariums. The use of antibiotics wound healing is not preferred due to the development antibiotic resistance bacteria and for economic reasons. This study was conducted demonstrate efficacy Juniperus root extract experimentally induced incised wounds yellowtail acei. Wound closure time, morphology, malondialdehyde content, glutathione s-transferase activity, changes gene expression levels, non-dispersibility aqueous environment,...

In this study, symptomatic fish samples were taken from rainbow trout farms. Isolation and identification of agents isolated made. DNA isolations different purified colonies carried out with the mericon bacterial kit. Real-Time PCR procedure was performed by using universal primers. Molecular identifications blasting nucleotides obtained sequence analysis amplicons. Spectrophotometric measurements at 412 nm wavelengths for AChE activity BChE liver brain tissues samples. The differences...

The aim of this study was to determine the zoonotic bacteria in carp farms Northern Iraq-Erbil region. Carp is main fish species cultured Erbil most common generally seen are Aeromonas hydrophila, Pseudomonas fluorescens and Streptococcus iniae. Samples were collected from 25 Six samples each farm. Head kidney intestine tissue sample. Then head pooled separately Total bacterial DNA had been extracted intestinal samples. pathogen Primers originally designed 16S RNA gene Zoonotic scanned all...

Objective: This report focuses on the diagnosis of rainbow trout mortality in Sarı Mehmet Dam Lake. Material and Methods: The ten (Oncorhynchus mykiss) (300 - 350 g) examined were infested with numerous copepod parasites which attached to body muscles especially caudal abdominal area. Results: According morphological results, this parasite was identified as L. cyprinacea. Histopathological examination lesions revealed inflammatory response dermis skeletal infectious granuloma. Conclusion:...

Bu çalışmada kültüre alınmış olan L. garvieae izolatları aynı besiyeri (TSA) ve miktarı (20ml) ile çevresel şartlarda (7,4 pH, 21oC, 24 saat) inkübe edilmiştir. İzolatların identifikasyonları Real-Time PCR 16S rRNA gen bölgesine spesifik primer assay teyit saatlik inkübasyon periyodunun ardından 10ml’lik TSB besiyerinde gelişen izolatlar 600 nm dalga boyunda 1.0 OD’ye eşitlenmiştir. Farklı gruplardan DNA izolasyonları; sıvı kaynatma, katı (Eşit OD) 2 farklı ticari kit kullanılarak manuel...