A5089063131- DNA Repair Mechanisms
- Advanced Electron Microscopy Techniques and Applications
- Enzyme Structure and Function
- Photosynthetic Processes and Mechanisms
- Advanced Polymer Synthesis and Characterization
- Polymer Surface Interaction Studies
- Cellular transport and secretion
- DNA and Nucleic Acid Chemistry
- Electron and X-Ray Spectroscopy Techniques
- Microtubule and mitosis dynamics
- Advanced Fluorescence Microscopy Techniques
- Calcium signaling and nucleotide metabolism
- Photopolymerization techniques and applications
- Lipid Membrane Structure and Behavior
- Marine Biology and Environmental Chemistry
- Antimicrobial agents and applications
- Photonic and Optical Devices
- Digital Holography and Microscopy
- Pickering emulsions and particle stabilization
- Advanced X-ray Imaging Techniques
- Toxin Mechanisms and Immunotoxins
- Cell Adhesion Molecules Research
- Photonic Crystals and Applications
- Mesoporous Materials and Catalysis
- Cancer-related Molecular Pathways
Memorial Sloan Kettering Cancer Center
2019-2021
Howard Hughes Medical Institute
2019
Materials Science & Engineering
2017
Beijing University of Chemical Technology
2012-2017
Institute of Biophysics
2014-2017
Chinese Academy of Sciences
2014-2017
University of Chinese Academy of Sciences
2016
Electron tomography (ET) plays an important role in revealing biological structures, ranging from macromolecular to subcellular scale. Due limited tilt angles, ET reconstruction always suffers the ‘missing wedge’ artifacts, thus severely weakens further interpretation. In this work, we developed algorithm called Iterative Compressed-sensing Optimized Non-uniform fast Fourier transform (ICON) based on theory of compressed-sensing and assumption sparsity specimens. ICON can significantly...
Significance We report on X-ray and cryo-EM structural studies the assembly of human Shieldin complex composed SHLD2, SHLD3, REV7, as well its with bound TRIP13 toward understanding principles underlying TRIP13-mediated disassembly Shieldin. Our identify a conformational heterodimeric alignment open (O) closed (C) conformers REV7 when to fused SHLD2–SHLD3 construct. The AAA + ATPase captures N terminus C-REV7 (REV NT ) within central hexameric channel, rotatory motion associated sequential...
Electron tomography (ET) combining subsequent sub-volume averaging has been becoming a unique way to study the in situ 3D structures of macromolecular complexes. However, information missing electron due limited angular sampling is still bottleneck high-resolution application. Here, based on understanding smooth nature biological specimen, we present new iterative image reconstruction algorithm, FIRT (filtered technique) for by algebra technique (ART) and nonlinear diffusion (ND) filter...
Particles with a hard body and soft, clickable dimple- or bulge-patches are prepared by simple combined chain- step-growth dispersion polymerization.
Studies on coat protein I (COPI) have contributed to a basic understanding of how proteins generate vesicles initiate intracellular transport. The core component the COPI complex is coatomer, which multimeric that needs be recruited from cytosol membrane in order function bending and cargo sorting. Previous structural studies clathrin adaptors found recruitment induces large conformational change promoting their role Here, pursuing negative-stain electron microscopy coupled with...
Correlative cryo-fluorescence and cryo-electron microscopy (cryo-CLEM) system has been fast becoming a powerful technique with the advantage to allow fluorescent labeling direct visualization of close-to-physiologic ultrastructure in cells at same time, offering unique insights into specific cellular function. There have various engineered ways achieve cryo-CLEM including commercial FEI iCorr that integrates fluorescence microscope column transmission electron microscope. In this study, we...
ABSTRACT The Fanconi Anemia (FA) pathway is essential for the repair of DNA interstrand crosslinks (ICLs). activated when a replication fork stalls because an ICL or other stress. A central event in activation mono-ubiquitination FANCI-FANCD2 (ID) complex by FA Core complex, ubiquitin ligase nine subunits. Here we describe cryo-EM structures 1.1 MDa at 3.1 angstroms, except FANCA subunit 3.4, and containing Core, ID UBE2T conjugating enzyme 4.2 angstroms. has unusual stoichiometry with two...
ABSTRACT The FANCI-FANCD2 (ID) complex, mutated in the Fanconi Anemia (FA) cancer predisposition syndrome, is required for repair of replication forks stalled at DNA interstrand crosslinks (ICL) and related lesions 1 . FA pathway activated when two converge onto an ICL 2 , triggering mono-ubiquitination ID complex. essential by excision, translesion synthesis homologous recombination, but its function was hitherto unknown 1,3 Here, 3.48 Å cryo-EM structure mono-ubiquitinated (ID Ub ) bound...