A5054190726- DNA Repair Mechanisms
- Enzyme Structure and Function
- Lipid metabolism and biosynthesis
- Protein Degradation and Inhibitors
- Extracellular vesicles in disease
- Clostridium difficile and Clostridium perfringens research
- Antimicrobial Resistance in Staphylococcus
- DNA and Nucleic Acid Chemistry
- Viral gastroenteritis research and epidemiology
- Venous Thromboembolism Diagnosis and Management
- Toxin Mechanisms and Immunotoxins
- Liver physiology and pathology
- Advanced Electron Microscopy Techniques and Applications
- Pulmonary Hypertension Research and Treatments
- Zebrafish Biomedical Research Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced biosensing and bioanalysis techniques
- Hormonal Regulation and Hypertension
- Cancer-related Molecular Pathways
- MicroRNA in disease regulation
- Advanced Proteomics Techniques and Applications
- Infant Nutrition and Health
- Eicosanoids and Hypertension Pharmacology
- Photosynthetic Processes and Mechanisms
- Single-cell and spatial transcriptomics
Rockefeller University
2024-2025
Memorial Sloan Kettering Cancer Center
2019-2021
The Graduate Center, CUNY
2019-2020
City University of New York
2020
Howard Hughes Medical Institute
2019
CUNY Advanced Science Research Center
2019
Universidade Estadual de Maringá
2013
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2013
Essen University Hospital
2013
University of California, San Diego
2013
Targeting Clostridium difficile infection is challenging because treatment options are limited, and high recurrence rates common. One reason for this that hypervirulent C. strains often have a binary toxin termed the toxin, in addition to enterotoxins TsdA TsdB. The has an enzymatic component, CDTa, pore-forming or delivery subunit CDTb. CDTb was characterized here using combination of single-particle cryoelectron microscopy, X-ray crystallography, NMR, other biophysical methods. In absence...
ABSTRACT Lipid droplets are fat storage organelles composed of a protein envelope and lipid‐rich core. Regulation this underlies differential lipid droplet formation function. In melanoma, has been linked to tumor progression metastasis, but it is unknown whether proteins play role. To address this, we performed proteomic analysis the in melanoma. We found that were differentially enriched distinct melanoma states; from melanocytic undifferentiated. DHRS3, which converts all‐trans‐retinal...
Abstract Circulating extracellular vesicles and particles (EVPs) are being investigated as potential biomarkers for early cancer detection, prognosis, disease monitoring. However, the suboptimal purity of EVPs isolated from peripheral blood plasma has posed a challenge in‐depth analysis EVP proteome. Here, we compared effectiveness different methods isolating healthy donor plasma, including ultracentrifugation (UC)‐based protocols, phosphatidylserine‐Tim4 interaction‐based affinity capture...
ABSTRACT The Fanconi Anemia (FA) pathway is essential for the repair of DNA interstrand crosslinks (ICLs). activated when a replication fork stalls because an ICL or other stress. A central event in activation mono-ubiquitination FANCI-FANCD2 (ID) complex by FA Core complex, ubiquitin ligase nine subunits. Here we describe cryo-EM structures 1.1 MDa at 3.1 angstroms, except FANCA subunit 3.4, and containing Core, ID UBE2T conjugating enzyme 4.2 angstroms. has unusual stoichiometry with two...
ABSTRACT The FANCI-FANCD2 (ID) complex, mutated in the Fanconi Anemia (FA) cancer predisposition syndrome, is required for repair of replication forks stalled at DNA interstrand crosslinks (ICL) and related lesions 1 . FA pathway activated when two converge onto an ICL 2 , triggering mono-ubiquitination ID complex. essential by excision, translesion synthesis homologous recombination, but its function was hitherto unknown 1,3 Here, 3.48 Å cryo-EM structure mono-ubiquitinated (ID Ub ) bound...
Summary Lipid droplets are fat storage organelles composed of a protein envelope and lipid rich core. Regulation this underlies differential droplet formation function. In melanoma, has been linked to tumor progression metastasis, but it is unknown whether proteins play role. To address this, we performed proteomic analysis the in melanoma. We found that were differentially enriched distinct melanoma states; from melanocytic undifferentiated. DHRS3, which converts all-trans-retinal...
Physiological maturity impacts seed quality through various mechanisms including vigor, desiccation tolerance, dormancy induction, synthesis of raw materials (including storage proteins), and the reorganization metabolisms. Peanut development can be classified into seven classes with four incremental stages per class. Based on mesocarp color, final three are commonly referred to as “orange”, “brown”, “black”. In 2017, freshly harvested pods from one genotype runner market-type peanuts grown...
ABSTRACT Protein monoaminylation is a class of posttranslational modification (PTM) that contributes to transcription, physiology and behavior. While recent analyses have focused on histones as critical substrates monoaminylation, the broader repertoire monoaminylated proteins in brain remains unclear. Here, we report development/implementation chemical probe for bioorthogonal labeling, enrichment proteomics-based detection dopaminylated brain. We identified 1,557 – many synaptic including...
Abstract Targeting Clostridium difficile infection (CDI) is challenging because treatment options are limited, and high recurrence rates common. One reason for this that hypervirulent CDI often has a binary toxin termed the C. (CDT), in addition to enterotoxins TsdA TsdB. CDT an enzymatic component, CDTa, pore-forming or delivery subunit CDTb. CDTb was characterized here using combination of single particle cryoEM, X-ray crystallography, NMR, other biophysical methods. In absence two novel...