A5010433561- Cellular Mechanics and Interactions
- Melanoma and MAPK Pathways
- Cell Adhesion Molecules Research
- Cancer-related Molecular Pathways
- Microtubule and mitosis dynamics
- Protein Tyrosine Phosphatases
- Microfluidic and Bio-sensing Technologies
- Extracellular vesicles in disease
- Cancer Cells and Metastasis
- Graphene and Nanomaterials Applications
- 3D Printing in Biomedical Research
- Monoclonal and Polyclonal Antibodies Research
- Colorectal Cancer Treatments and Studies
- Receptor Mechanisms and Signaling
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Reproductive Biology and Fertility
- Ubiquitin and proteasome pathways
- Nanoplatforms for cancer theranostics
- Molecular Communication and Nanonetworks
- Cell Image Analysis Techniques
- Phagocytosis and Immune Regulation
- Cancer, Hypoxia, and Metabolism
- Nicotinic Acetylcholine Receptors Study
Memorial Sloan Kettering Cancer Center
2015-2025
Kettering University
2021-2025
New York Proton Center
2012-2025
New York Structural Biology Center
2023
Maastricht University Medical Centre
2013
Inserm
2013
Akdeniz University
2013
Herlev Hospital
2013
Copenhagen University Hospital
2013
Université de Montpellier
2013
Tunneling nanotubes are long, non-adherent F-actin-based cytoplasmic extensions which connect proximal or distant cells and facilitate intercellular transfer. The identification of has been limited to cell lines, their role in cancer remains unclear. We detected tunneling mesothelioma lines primary human cells. Using a low serum, hyperglycemic, acidic growth medium, we stimulated nanotube formation bidirectional transfer vesicles, proteins, mitochondria between Notably, developed malignant...
Cohesin complex members have recently been identified as putative tumor suppressors in hematologic and epithelial malignancies. The cohesin guides chromosome segregation; however, mutant leukemias do not show genomic instability. We hypothesized that reduced function alters chromatin structure disrupts cis-regulatory architecture of hematopoietic progenitors. investigated the consequences Smc3 deletion normal malignant hematopoiesis. Biallelic loss induced bone marrow aplasia with premature...
Tunneling nanotubes are actin-based cytoplasmic extensions that function as intercellular channels in a wide variety of cell types.There is renewed and keen interest the examination modes communication cells all types, especially field cancer biology. -which literature have also been referred to "membrane nanotubes," "'intercellular' or 'epithelial' bridges," "cytoplasmic extensions" - under active investigation for their role facilitating direct communication. These structures not, until...
Fibrillar carbon nanotubes simultaneously deliver two small interfering RNAs, which safely prevent acute kidney injury and prolong survival in mice.
Immune cells have intensely physical lifestyles characterized by structural plasticity and force exertion. To investigate whether specific immune functions require stereotyped mechanical outputs, we used super-resolution traction microscopy to compare the synapses formed cytotoxic T with contacts other cell subsets macrophages. were globally compressive, which was fundamentally different from pulling pinching associated macrophage phagocytosis. Spectral decomposition of exertion patterns...
Mutated forms of the RAS oncogene drive 30% all cancers, but they cannot be targeted therapeutically using currently available drugs. The molecular and cellular mechanisms that create a heterogenous tumor environment harboring both mutant wild-type have not been elucidated. In this study, we examined horizontal transfer KRAS (Kirsten Rat Sarcoma Virus) between colorectal cancer (CRC) cells via direct form cell-to-cell communication called tunneling nanotubes (TNTs). TNT formation was...
Trastuzumab deruxtecan (T-DXd) is a human epidermal growth factor receptor 2 (HER2)-targeting antibody drug conjugate that has remarkable activity in HER2-positive cancers. However, the degree of benefit T-DXd not uniform among solid tumors even with high levels HER2. Despite HER2 expression, HER2/T-DXd complex may always undergo internalization and payload release dependent on receptor's conformation context. We hypothesize (EGFR), dimerization partner HER2, can modulate trafficking through...
Immunofluorescent staining is an informative tool that widely used in basic research. Automation of immunostaining improves reproducibility and quality the results. Up to now, use automation immunofluorescent was mostly limited one marker. Here we present tyramide signal amplification based method multiple marker detection, including detection antibodies, raised same species, tissue sections cultured cells. This can be beneficial for both clinical
Abstract Single-wall carbon nanotubes present unique opportunities for drug delivery, but have not advanced into the clinic. Differential nanotube accretion and clearance from critical organs been observed, mechanism fully elucidated. The liver has a complex cellular composition that regulates range of metabolic functions coincidently accumulates most particulate drugs. Here we provide unexpected details hepatic processing covalently functionalized including receptor-mediated endocytosis,...
Abstract Changes in the elastic properties of living tissues during normal development and pathological processes are often due to modifications collagen component extracellular matrix at various length scales. Force volume AFM can precisely capture mechanical biological samples with force sensitivity spatial resolution. The integration data molecular composition contributes understanding interplay between tissue biochemistry, organization function. detection micrometer-size, heterogeneous...
Abstract BRAFV600E mutations occur in 46% of melanomas and drive high levels ERK activity ERK-dependent proliferation. However, is insufficient to melanoma genetically engineered mouse models, 82% human benign nevi harbor mutations. We found that inhibited mesenchymal migration by causing feedback inhibition RAC1 activity. pathway induced activation restored invasion. In cells with BRAFV600E, mutant or PTEN inactivation cell motility. Together, these lesions occurred 26% Thus, although...
The cholinergic interneurons (ChIs) of the nucleus accumbens (NAc) have a critical role in activity this region, specifically context major depressive disorder. To understand circuitry regulating behavior, we sought to determine areas that directly project these by utilizing monosynaptic cell-specific tracing technique. Mapping showed projections are exclusive NAc ChIs. if some altered depression mouse model, used mice do not express calcium-binding protein p11 ChIs (ChAT-p11 cKO) and...
<div>Abstract<p><i>BRAF</i><sup><i>V600E</i></sup> mutations occur in 46% of melanomas and drive high levels ERK activity ERK-dependent proliferation. However, BRAF<sup>V600E</sup> is insufficient to melanoma genetically engineered mouse models, 82% human benign nevi harbor <i>BRAF</i><sup><i>V600E</i></sup> mutations. We found that inhibited mesenchymal migration by causing feedback inhibition...
<p>Confocal Z-stack image of cells treated with DMSO</p>
<p>Figure S1,S2,S3 &S4</p>
<p>Figure S5 & S6</p>
<p>Confocal Z-stack image of cells treated with MEK inhibitor</p>
<p>Time-lapse video with and without ERK inhibition</p>
Upon spinal cord injury, axons attempting to regenerate need overcome the repulsive actions of myelin-associated inhibitors, including glycoprotein, Nogo-A, and oligodendrocyte myelin glycoprotein. These inhibitors bind signal through a neuronal receptor/co-receptor/transducer complex composed NgR1, Lingo-1, p75. Consequently, p75 is cleaved by alpha secretase followed gamma-secretase, triggering downstream signaling that inhibits axonal regrowth. ADAM10 ADAM17 are both known function as...
SUMMARY ANSWERThe absence of a functional p27 leads to dramatic increase in the number multi-oocyte follicles (MOFs) juvenile female mice; would promote individualization favoring development fertile eggs.