A5049043665- Adipose Tissue and Metabolism
- Heart Rate Variability and Autonomic Control
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Regulation of Appetite and Obesity
- Genetic Syndromes and Imprinting
- Single-cell and spatial transcriptomics
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Biochemical Analysis and Sensing Techniques
- Tryptophan and brain disorders
- Cardiac Health and Mental Health
- Neuroinflammation and Neurodegeneration Mechanisms
- Animal Genetics and Reproduction
- Cell Image Analysis Techniques
- Genetics and Neurodevelopmental Disorders
- Toxin Mechanisms and Immunotoxins
- Receptor Mechanisms and Signaling
- Diet and metabolism studies
- Vagus Nerve Stimulation Research
- Adipokines, Inflammation, and Metabolic Diseases
Egas Moniz - Cooperativa de Ensino Superior Crl
2022
A4F-Algafuel (Portugal)
2022
Universidade Nova de Lisboa
2021
Instituto Gulbenkian de Ciência
2015-2020
Abstract Conditional expression of diphtheria toxin receptor (DTR) is widely used for tissue-specific ablation cells. However, (DT) crosses the blood–brain barrier, which limits its utility ablating peripheral cells using Cre drivers that are also expressed in central nervous system (CNS). Here we report development a brain-sparing DT, termed BRAINSPAReDT, genetic outside CNS. We prevent barrier passage DT through PEGylation, polarizes molecule and increases size. validate BRAINSPAReDT with...
Anti-obesity drugs in the amphetamine (AMPH) class act brain to reduce appetite and increase locomotion. They are also characterized by adverse cardiovascular effects with origin that, despite absence of any vivo evidence, is attributed a direct sympathomimetic action heart. Here, we show that cardiac side AMPH originate from can be circumvented PEGylation (PEGyAMPH) exclude its central action. PEGyAMPH does not enter facilitates SNS activity via theβ2-adrenoceptor, protecting mice against...
Evaluating the epigenetic landscape in stem cell compartment at single-cell level is essential to assess cells' heterogeneity and predict their fate. Here, using a genome-wide transcriptomics approach vivo, we evaluated allelic expression imbalance progeny of single hematopoietic cells (HSCs) as read-out marking. After 4 months extensive proliferation differentiation, found that X-chromosome inactivation (XCI) tightly maintained all single-HSC derived cells. In contrast, vast majority...
To read the full abstract: Cell Metab. 2020 Jun 2;31(6):1120-1135.e7. doi: 10.1016/j.cmet.2020.04.013.
ABSTRACT The persistence of patterns monoallelic expression is a controversial matter. We report genome-wide in vivo transcriptomics approach based on allelic imbalance to evaluate whether the transcriptional single murine hematopoietic stem cells (HSC) are still present respective differentiated clonal B-cell populations. For 14 genes, we show conclusive evidence for remarkable HSC-derived B allele-specific autosomal states already HSCs. In striking contrast frequency genes with differences...