A5061177531- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Cell Adhesion Molecules Research
- Biotin and Related Studies
- Immune Cell Function and Interaction
- Receptor Mechanisms and Signaling
- Chemical Synthesis and Analysis
- Platelet Disorders and Treatments
- HER2/EGFR in Cancer Research
- Caveolin-1 and cellular processes
- Advanced Biosensing Techniques and Applications
- Click Chemistry and Applications
Harvard University
2019-2024
Boston VA Research Institute
2020-2024
University of California, San Francisco
2023
Signaling through the CD19-CD81 co-receptor complex, in combination with B cell receptor, is a critical determinant of development and activation. It unknown how CD81 engages CD19 to enable function. Here, we report 3.8-angstrom structure complex bound therapeutic antigen-binding fragment, determined by cryo-electron microscopy (cryo-EM). The includes both extracellular domains transmembrane helices revealing contact interface between ectodomains that drives formation. Upon binding CD19,...
CD81 and its binding partner CD19 are core subunits of the B cell co-receptor complex. While belongs to extensively studied Ig superfamily, a poorly understood family four-pass transmembrane proteins called tetraspanins. Tetraspanins play important physiological roles by controlling protein trafficking other processes. Here, we show that relies on ectodomain traffic surface. Moreover, anti-CD81 antibody 5A6, which binds selectively activated cells, recognizes conformational epitope is masked...
Antibodies are central mediators of the adaptive immune response, and they powerful research tools therapeutics. Antibody discovery requires substantial experimental effort, such as immunization campaigns or in vitro library screening. Predicting antibody-antigen binding a priori remains challenging. However, recent machine learning methods raise possibility silico antibody discovery, bypassing reducing initial bottlenecks. Here, we report virtual screen using AlphaFold-Multimer (AF-M) that...
Photo-proximity labeling proteomics (PLP) methods have recently shown that the cell surface receptors can dynamically form lateral interactome networks. Here, we present a paired set of PLP workflows simultaneously track neighborhood changes for oncogenic epidermal growth factor receptor (EGFR) with temporal resolution, both outside and inside cells. We achieved this by augmenting multiscale workflow call MultiMap, where three photo-probes different ranges were photo-activated one...
Abstract The B cell receptor (BCR) signals together with a multi-component co-receptor complex to initiate activation in response antigen binding. This process underlies nearly every aspect of proper function. Here, we take advantage peroxidase-catalyzed proximity labeling combined quantitative mass spectrometry track signaling dynamics from 10 seconds 2 hours after BCR stimulation. approach enables tracking 2,814 proximity-labeled proteins and 1,394 quantified phosphosites provides an...
SUMMARY CD81 and its binding partner CD19 are core subunits of the B cell co-receptor complex. While is a single-pass transmembrane protein belonging to extensively studied Ig superfamily, belongs conserved but poorly understood family four-pass proteins called tetraspanins. These functionally diverse play important roles in wide variety different organ systems by controlling trafficking other cellular processes. Here, we show that relies on ectodomain control surface. Moreover, anti-CD81...