A5034568574- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Neuroendocrine Tumor Research Advances
- Lung Cancer Research Studies
- Lung Cancer Treatments and Mutations
- RNA Research and Splicing
- Radiomics and Machine Learning in Medical Imaging
- Cytokine Signaling Pathways and Interactions
- Cancer-related gene regulation
- Neuroscience of respiration and sleep
- Pancreatic and Hepatic Oncology Research
- Cancer Research and Treatments
- Cancer Cells and Metastasis
- Cancer Mechanisms and Therapy
- Cancer, Hypoxia, and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Metalloenzymes and iron-sulfur proteins
- Colorectal Cancer Treatments and Studies
- Melanoma and MAPK Pathways
- Erythrocyte Function and Pathophysiology
- Parkinson's Disease Mechanisms and Treatments
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Pancreatic function and diabetes
- Neuroblastoma Research and Treatments
University of California, San Francisco
2020-2025
Henan University of Technology
2022-2025
National Taipei University
2024
National Taiwan University
2023-2024
UCSF Helen Diller Family Comprehensive Cancer Center
2022-2024
Chan Zuckerberg Initiative (United States)
2024
National Yang Ming Chiao Tung University
2010-2023
Institute of Biomedical Science
2023
National Chiayi University
2021-2022
Memorial Sloan Kettering Cancer Center
2019-2020
Receptor tyrosine kinase (RTK)-mediated activation of downstream effector pathways such as the RAS GTPase/MAP (MAPK) signaling cascade is thought to occur exclusively from lipid membrane compartments in mammalian cells. Here, we uncover a membraneless, protein granule-based subcellular structure that can organize RTK/RAS/MAPK cancer. Chimeric (fusion) oncoproteins involving certain RTKs including ALK and RET undergo de novo higher-order assembly into membraneless cytoplasmic granules...
Abstract Targeted therapy is effective in many tumor types including lung cancer, the leading cause of cancer mortality. Paradigm defining examples are targeted therapies directed against non-small cell (NSCLC) subtypes with oncogenic alterations EGFR, ALK and KRAS. The success limited by drug-tolerant persister cells (DTPs) which withstand adapt to treatment comprise residual disease state that typical during clinical therapies. Here, we integrate studies patient-derived immunocompetent...
Molecularly targeted cancer therapy has improved outcomes for patients with targetable oncoproteins, such as mutant EGFR in lung cancer. Yet, the long-term survival of these remains limited, because treatment responses are typically incomplete. One potential explanation lack complete and durable is that oncogene-driven cancers activating mutations often harbor additional co-occurring genetic alterations. This hypothesis untested most alterations co-occur EGFR. Here, we report functional...
microRNAs (miRNAs) are endogenous short RNAs that mediate vast networks of post-transcriptional gene regulation. Although computational searches and experimental profiling provide evidence for hundreds functional targets individual miRNAs, such data rarely clear insight into the phenotypic consequences manipulating miRNAs in vivo. We describe a genome-wide collection 165 Drosophila miRNA transgenes find majority induced specific developmental defects, including phenocopies mutants myriad...
Aim: Few studies have examined the prevalence of restless legs syndrome (RLS) in Asian populations, with existing data suggesting substantially lower rates RLS populations compared Caucasians. However, varying definitions as well problematic methodology make conclusions about difficult to interpret. The current study therefore examines Taiwanese adults. Methods: Subjects were 4011 residents over age 15 years. Data was collected using a computer‐assisted telephone interviewing (CATI) system...
Alveolar formation increases the surface area for gas-exchange and is key to physiological function of lung. epithelial cells, myofibroblasts endothelial cells undergo coordinated morphogenesis generate folds (secondary septa) form alveoli. A mechanistic understanding alveologenesis remains incomplete. We found that planar cell polarity (PCP) pathway required in alveolar mammals. Our studies uncovered a
Circadian pacemaker circuits consist of ensembles neurons, each expressing molecular oscillations, but how circuit-wide coordination multiple oscillators regulates rhythmic physiological and behavioral outputs remains an open question. To investigate the relationship between pattern oscillator phase throughout circadian circuit locomotor activity rhythms in Drosophila , we perturbed electrical pigment dispersing factor (PDF) levels lateral ventral neurons (LNv) assayed their combinatorial...
The deregulated nonoxidative pentose phosphate pathway (PPP) is known to promote oncogenesis, but the molecular mechanism remains unknown. Here, we report that human ribose-5-phosphate isomerase A (RPIA) plays a role in hepatocellular carcinoma (HCC). significant increase RPIA expression was detected both tumor biopsies of HCC patients and liver cancer tissue array. Importantly, clinicopathological analysis indicated mRNA levels were highly correlated with clinical stage, grade, size, types,...
Obstructive sleep apnea (OSA) is associated with cognitive impairment and neuronal injury. Long-term exposure to intermittent hypoxia (LTIH) in rodents, modeling the oxygenation patterns apnea, results NADPH oxidase 2 (Nox2) oxidative injury many populations. Brainstem motoneurons susceptible LTIH show uncompensated endoplasmic reticulum stress responses increased (CCAAT/enhancer binding protein homologous (CHOP). We hypothesized that CHOP underlies In this series of studies, we first...
Altered metabolism is one of the hallmarks cancers. Deregulation ribose-5-phosphate isomerase A (RPIA) in pentose phosphate pathway (PPP) known to promote tumorigenesis liver, lung, and breast tissues. Yet, molecular mechanism RPIA-mediated colorectal cancer (CRC) unknown. Our study demonstrates a noncanonical function RPIA CRC. Data from mRNAs 80 patients' CRC tissues paired nontumor protein levels, as well tissue array, indicate significantly elevated modulates cell proliferation...
Alzheimer's disease (AD) is a neurodegenerative disorder characterized by cognitive impairment, memory loss, and behavioral deficits. β-amyloid1–42 (Aβ1–42) aggregation significant cause of the pathogenesis in AD. Despite numerous types research, current treatment efficacy remains insufficient. Hence, novel therapeutic strategy required. Nitric oxide (NO) multifunctional gaseous molecule. NO displays neuroprotective role central nervous system inhibiting Aβ rescuing learning deficit through...
Abstract Background Enterotoxigenic Escherichia coli (ETEC) is one of the primary causes diarrhea in piglets, creating substantial economic losses swine farming industry worldwide. This study aimed to investigate impacts zinc caproate (ZnCA, C 12 H 22 O 4 Zn) on intestinal health, growth performance, inflammatory status, and Zn homeostasis weaned piglets challenged with ETEC K88. In total, 48 (Duroc × Landrace Yorkshire, 7.78 ± 0.19 kg, 28 d) were selected for a 21-d experiment. Each...
Abstract Somatic BRAF alterations occur in up to 5% of non-small cell lung cancer (NSCLC) cases and comprise three mechanistically distinct molecular subtypes: Class I (e.g., V600E) function as constitutively active monomers, II that signal RAS-independent mutant homodimers, III activate MAPK signaling via RAS-dependent BRAF/wild-type RAF heterodimers. While V600E (Class I) mutations are effectively targeted by approved therapies against these oncogenic there no established treatments for...
Abstract Molecularly targeted therapies have significantly improved clinical outcomes by directly addressing oncogenic driver mutations in genes such as EGFR. However, despite their initial success, long-term patient survival remains limited due to the emergence of drug tolerance and resistance. This study investigates molecular mechanisms underlying response non-small cell lung cancer (NSCLC). Using single-cell RNA sequencing oncogene-driven human NSCLCs at residual disease (RD) state...
Craniofacial structure is an important determinant of obstructive sleep apnoea (OSA) syndrome risk. Three-dimensional stereo-photogrammetry (3dMD) a novel technique which allows quantification the craniofacial profile. This study compares facial images OSA patients captured by 3dMD to three-dimensional computed tomography (3-D CT) and two-dimensional (2-D) digital photogrammetry. Measurements were correlated with indices severity.Thirty-eight diagnosed included, photogrammetry, 3-D CT...
α-1,2 mannosidases, key enzymes in N-glycosylation, are required for the formation of mature glycoproteins eukaryotes. Aberrant regulation mannosidases can result cancer, although underlying mechanisms unclear. Here, we report distinct roles mannosidase subtypes (MAN1A, MAN1B, ERMAN1, MAN1C) hepatocellular carcinoma (HCC). Clinicopathological analyses revealed that clinical stage, tumor size, α-fetoprotein level, and invasion status were positively correlated with expression levels MAN1A1,...
Dysregulation of the enzymes involved in pentose phosphate pathway (PPP) is known to promote tumorigenesis. Our recent study demonstrated that ribose-5-phosphate isomerase (RPIA), a key regulator PPP, regulates hepatoma cell proliferation and colony formation. studies zebrafish reveal RPIA-mediated hepatocarcinogenesis requires extracellular signal-regulated kinase (ERK) β-catenin signaling. To further investigate hepatocarcinogenesis, two independent lines transgenic expressing human RPIA...
In Drosophila, Dicer-1 binds Loquacious-PB (Loqs-PB) as its major co-factor. Previous analyses indicated that loqs mutants only partially impede miRNA processing, but the activity of minor isoforms or maternally deposited Loqs was not eliminated in these studies. We addressed this by generating a cell line from loqs-null embryos and found ∼40% miRNAs showed clear dependence. Genome-wide comparison hairpin structure dependence suggested substrates are influenced base-pairing status at dicing...
The narrowing pattern of the upper airway in obstructive sleep apnea patients may be different as compared with awake. Three types obstruction were observed these subjects during drug-induced sleep. suggests that strategies should selected management.To identify sites and evaluate dynamic movement syndrome (OSAS) while awake asleep.This study included 10 treated for OSAS between August 2003 June 2004. Overnight polysomnography was performed on all patients. Parameters including gender, age,...
Abstract Forty years ago, a high frequency of lethal giant larvae (lgl) alleles in wild populations Drosophila melanogaster was reported. This locus has been intensively studied for its roles epithelial polarity, asymmetric neural divisions, and restriction tissue proliferation. Here, we identify lgl the Bloomington second chromosome deficiency kit University California at Los Angeles Bruinfly FRT40A-lethal P collection. These unrecognized aberrations confound use these workhorse collections...