A5008339868- Autophagy in Disease and Therapy
- Mesenchymal stem cell research
- Parkinson's Disease Mechanisms and Treatments
- Ubiquitin and proteasome pathways
- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Transgenic Plants and Applications
- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
- Nerve injury and regeneration
- Endoplasmic Reticulum Stress and Disease
- PARP inhibition in cancer therapy
- Toxin Mechanisms and Immunotoxins
- Foreign Body Medical Cases
Peking University People's Hospital
2025
Peking University
2025
Sichuan University
2009-2018
During proteasomal stress, cells can alleviate the accumulation of polyubiquitinated proteins by targeting them to perinuclear aggresomes for autophagic degradation, but mechanism underlying activation this compensatory pathway remains unclear. Here we report that PINK1-s, a short form Parkinson disease (PD)-related protein kinase PINK1 (PTEN induced putative 1), is major regulator aggresome formation. PINK1-s extremely unstable due its recognition N-end rule pathway, and tends accumulate in...
Ribosome-inactivating proteins (RIPs) are a family of enzymes that depurinate rRNA and inhibit protein biosynthesis. Here we report the purification, apoptosis-inducing activity, polyethylene glycol (PEG) modification RIP from bitter melon seeds. The has homogenous N-terminal sequence NAsp- Val-Ser-Phe-Arg. Moreover, displayed strong activity suppressed cancer cell growth. This might be attributed to activation caspases-3. To make it available for in vivo application, immunogenicity was...
Aggresome formation is a major strategy to enable cells cope with proteasomal stress. Misfolded proteins are assembled into micro-aggregates and transported the microtubule organizing center (MTOC) form perinuclear aggresomes before their degradation through autophagy. So far, multiple factors have been identified as activators of micro-aggregate formation, but much less known about regulatory mechanisms transport. Here, we report that stress leads activation p38 MAPK family members. Two...