A5015116226- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- interferon and immune responses
- SARS-CoV-2 detection and testing
- RNA regulation and disease
- Extracellular vesicles in disease
- Animal Virus Infections Studies
- Herpesvirus Infections and Treatments
- Virus-based gene therapy research
- Biomedical and Engineering Education
- Biosensors and Analytical Detection
- Respiratory viral infections research
- COVID-19 Impact on Reproduction
- Cytomegalovirus and herpesvirus research
- RNA Interference and Gene Delivery
University of Pennsylvania
2019-2023
Hospital of the University of Pennsylvania
2022-2023
Children's Hospital of Philadelphia
2022-2023
University of Minnesota Medical Center
2023
Penn Center for AIDS Research
2021-2022
Coronaviruses are adept at evading host antiviral pathways induced by viral double-stranded RNA, including interferon (IFN) signaling, oligoadenylate synthetase–ribonuclease L (OAS-RNase L), and protein kinase R (PKR). While dysregulated or inadequate IFN responses have been associated with severe coronavirus infection, the extent to which recently emerged SARS-CoV-2 activates antagonizes these is relatively unknown. We found that infects patient-derived nasal epithelial cells, present...
Middle East respiratory syndrome coronavirus (MERS-CoV) is the second novel zoonotic to emerge in 21st century and cause outbreaks of severe disease. More than 2,200 cases 800 deaths have been reported date, yet there are no licensed vaccines or treatments. Coronaviruses encode unique accessory proteins that not required for replication but most likely play roles immune antagonism and/or pathogenesis. Our study describes functions MERS-CoV NS4a NS4b during infection a human airway-derived...
Significance Middle East respiratory syndrome coronavirus (MERS-CoV) causes highly lethal disease. MERS-CoV encodes innate immune antagonists, including accessory proteins NS4a and NS4b, unique to the merbeco lineage of betacoronaviruses, nsp15 protein endoribonuclease (EndoU), conserved among all coronaviruses. While mutation each antagonist alone has little effect on immunity, infections with recombinant MERS-CoVs mutations EndoU in combination either or NS4b activate signaling pathways...
Coronaviruses are adept at evading host antiviral pathways induced by viral double-stranded RNA, including interferon (IFN) signaling, oligoadenylate synthetase-ribonuclease L (OAS-RNase L), and protein kinase R (PKR). While dysregulated or inadequate IFN responses have been associated with severe coronavirus infection, the extent to which recently emerged SARS-CoV-2 activates antagonizes these is relatively unknown. We found that infects patient-derived nasal epithelial cells, present...
The widespread coronavirus disease 2019 (COVID-19) is caused by infection with the novel SARS-CoV-2. Currently, we have limited understanding of which cells become infected SARS-CoV-2 in human tissues and where viral RNA localizes on subcellular level. Here, present a platform for preparing autopsy tissue visualizing using fluorescence situ hybridization (FISH) amplification chain reaction. We developed probe sets that target different regions (including ORF1a N), as well specifically...
Community-based health care clinics and hospital outreach services have the potential to expand coronavirus disease 2019 (COVID-19) diagnostics rural areas. However, reduced specimen stability during extended transport, absence of a cold chain centralized laboratories, biosafety concerns surrounding handling limited this expansion. In following study, we evaluated eNAT (Copan Italia, Brescia, Italy) as an alternative transport system address challenges associated with expanding COVID-19...
Herpes simplex virus type-1 (HSV-1) protein ICP27 is an essential immediate early (IE) that promotes the expression of viral (E) and late (L) genes via multiple mechanisms. Our understanding this complex regulatory has been greatly enhanced by characterization HSV-1 mutants bearing engineered alterations in gene. However, much analysis performed interferon-deficient Vero monkey cells. Here, we assessed replication a panel several other cell types. shows lacking ICP27's amino (N)-terminal...
Abstract The widespread Coronavirus Disease 2019 (COVID-19) is caused by infection with the novel coronavirus SARS-CoV-2. Currently, we have a limited toolset available for visualizing SARS-CoV-2 in cells and tissues, particularly tissues from patients who died COVID-19. Generally, single-molecule RNA FISH techniques shown mixed results formalin fixed paraffin embedded such as those preserved human autopsies. Here, present platform preparing autopsy tissue using amplification hybridization...
Middle East respiratory syndrome coronavirus (MERS-CoV) emerged into humans in 2012, causing highly lethal disease. The severity of disease may be part because MERS-CoV is adept at antagonizing early innate immune pathways - interferon (IFN) production and signaling, protein kinase R (PKR), oligoadenylate synthetase ribonuclease L (OAS/RNase L) generated response to viral double-stranded (ds)RNA during genome replication. This contrast SARS-CoV-2, which we recently reported activates PKR...
Rotavirus (RV) was a common healthcare-associated infection prior to the introduction of RV vaccine. Following widespread vaccination, rotavirus cases are rare. We describe an investigation cluster infections in pediatric hospital which uncommon genotype not typically circulating United States detected.
Abstract Background Rotavirus group A (RVA) was the most common cause of infectious gastroenteritis among young children before introduction rotavirus vaccine in United States 2006. Following widespread vaccination, U.S. hospital acquired (HA) cases are rare. We describe a cluster infections pediatric with genotype uncommon children. Methods Patient HA gastrointestinal (GI) illness were detected through hospital-wide microbiology surveillance, performed by Infection Prevention and Control...