Electrochemiluminescence (ECL) is a highly successful technique used in commercial immunoassays for clinical diagnosis. Developing an ECL-based multiplex immunoassay, with the potential to enable high-throughput detection of multiple biomarkers simultaneously, remains current research interest yet limited by narrow choice ECL luminophores. Herein we report synthesis, photophysics, electrochemistry, and several new ruthenium(II) iridium(III) complexes, three which are eventually as signal...

By exposing an electrode surface with a latent fingerprint to electrochemiluminescence (ECL)-generating luminophore, ECL is produced from the not covered by fingerprint, generating negative image (see picture, left). The can also be pre-stained luminophores, which generates and yields positive (right). ITO=indium tin oxide. Detailed facts of importance specialist readers are published as ”Supporting Information”. Such documents peer-reviewed, but copy-edited or typeset. They made available...

Abstract Cell‐matrix adhesions play essential roles in a variety of biological processes. Herein, we report label‐free method to map cell‐matrix single living cells on an electrode surface by electrochemiluminescence (ECL). An indium tin oxide modified with silica nanochannel membrane was used as the substrate electrode, at which ECL generation from freely diffusing luminophores provided distinct visual contrast between adhesion sites and noncontacted domains, thus selectively revealing...

Outstanding photoluminescence (PL) and electroluminescence properties of quantum dots (QDs) promise possibilities for them to meet challenging expectations electrochemiluminescence (ECL), which at present relies on inefficient spectral-irresolvable emitters based transition-metal complexes (such as Ru(bpy)32+). However, ECL is reported be extremely sensitive the surface traps QDs likely because spatially temporally separated electrochemical charge injections. Results here reveal that, by...

Abstract Cell junctions are protein structures located at specific cell membrane domains that determine key processes in multicellular development. Here we report spatially selective imaging of by electrochemiluminescence (ECL) microscopy. By regulating the concentrations luminophore and/or co‐reactant, thickness ECL layer can be controlled to match with spatial location different junctions. At a low concentration luminophore, generation is confined electrode surface, thus revealing only...

Developing highly sensitive multiplex immunoassays is urgently needed to guide medical research and improve clinical diagnosis. Here, we report the proximity electrochemiluminescence (ECL) generation enabled by gold microbeads (GMBs) for improving detection sensitivity multiplexing capacity of ECL (ECLIAs). As demonstrated microscopy finite element simulation, GMBs can function as spherical ultramicroelectrodes triggering reactions in solutions. Employing solid carriers bead-based ECLIA,...

Abstract Herein we report the study of electrochemiluminescence (ECL) generation by tris(2,2′‐bipyridyl)ruthenium (Ru(bpy) 3 2+ ) and five tertiary amine coreactants. The ECL distance lifetime coreactant radical cations were measured self‐interference spectroscopy. And reactivity coreactants was quantitatively evaluated in terms integrated intensity. By statistical analysis images single Ru(bpy) ‐labeled microbeads, propose that codetermine emission intensity thus sensitivity immunoassay....

Tetracoordinate boron-based fluorescent materials have shown extensively applications in chemistry, biology and science owing to their unique optoelectronic properties. However, constructing chiral boron-stereogenic fluorophores through practical universal strategies remains rare challenging. Herein, as a proof of concept, we report an enantioselective postfunctionalization boron dipyrromethene dyes (BODIPYs), acess BODIPYs moderate good yields with commendable enantioselectivity. Chiral...

A totem-pole boost bridgeless power-factor-correction rectifier with simple zero-current detection and full-range zero-voltage switching (ZVS) is proposed, which operates at the boundary of discontinuous-conduction mode continuous-conduction mode. Comparing dual rectifier, required number power components reduced by one third two current transducers can be eliminated. The achieved sampling diode through a single transducer. Besides, soft-transition method proposed to suppress spike...

The aggregation-induced emission of tetraphenylethene was explored for enhancing the visualization latent fingerprints on wet non-porous surfaces.

Originated from analyzing nonactive power loss, a novel optimization method and modulation solution for bidirectional isolated dual-active-bridge (DAB) dc-dc converters are proposed in order to achieve high efficiency wide operating range. A comprehensive loss model is developed, including both the components delivered back source load. This paper points out that when minimum achieved, zero-voltage soft switching can be naturally fulfilled. The optimal phase-shift pair obtained by keep low...

An interleaved totem-pole boost bridgeless rectifier with reduced reverse-recovery problems for power factor correction is proposed in this paper. The converter consists of two and intercoupled cells. cells operate phase-shift mode. Thus, the input current can be continuous low ripple. For individual cells, they alternatively discontinuous mode maximum duty ratio 50%, which allows shifting diode di/dt rate to achieve zero-current switching off. Zero-voltage achieved MOSFETs under line input....

Molecular electrocatalysis for oxygen reduction at a polarized water/1,2-dichloroethane (DCE) interface was studied, involving aqueous protons, ferrocene (Fc) in DCE and amphiphilic cobalt porphyrin catalysts adsorbed the interface. The catalyst, (2,8,13,17-tetraethyl-3,7,12,18-tetramethyl-5-p-amino-phenylporphyrin) cobalt(II) (CoAP), functions like conventional porphyrins, activating O2 via coordination by formation of superoxide structure. Furthermore, due to hydrophilic nature aminophenyl...

Membranes with the ability of molecular/ionic separation offer potential in many processes ranging from molecular purification/sensing, to nanofluidics and mimicking biological membranes. In this work, we report preparation a perforative free-standing ultrathin silica membrane consisting straight parallel nanochannels uniform size (∼2.3 nm) for precise fast separation. Due its small channel size, exhibits selectivity toward molecules based on charge, which can be tuned by ionic strength, pH...

Oxygen (O2) is involved in many life activities, and its vivo monitoring of vital importance. In electrochemistry with carbon fiber microelectrode (CFME) has been proven to be a suitable technique, but the surface fouling propensity poses great challenge current stability reliability. Herein we electro-grafted silica nanoporous membrane (SNM) consisting uniform, closely packed, vertically aligned nanochannels on CFME surface, which was capable protecting effectively from biofouling and,...

The rapid and sensitive detection of nitroaromatic compounds is great significance for human health, the environment, public security. present work reports on extraction electrochemical analysis trace compounds, such as explosives organophosphate pesticides (OPs), using indium tin oxide (ITO) electrodes modified with a highly ordered aligned binary assembly silica mesochannels micelles (BASMM). With pore diameter ca. 2-3 nm, (SMs) perpendicularly oriented to ITO electrode surface can provide...

Thermosensation, the ability to detect environmental temperature change, is one of most ancient and crucial processes for survival all living organisms. Mammals use temperature-sensitive transient receptor potential (thermoTRP) cation channels as thermometers convert change into electrical signals that are finally received action potentials by nerve endings. In this work, we report bionic thermosensation solid-state hybrid nanochannels based on principle thermally sensitive permselective ion...

Abstract Electrochemiluminescence (ECL) is a powerful transduction technique in biosensing and diagnostics, while mechanistic studies are still scarce. Herein we report the combined use of microtube electrode (MTE) microscopy to measure thickness ECL layer (TEL) decipher reaction mechanisms. For classical system involving tris(2,2′‐bipyridyl)ruthenium tri‐ n ‐propylamine, pattern generated at MTE tends change from ring spot upon increasing luminophore concentration, with TEL varying ca. 3.1...

Here we report an electrochemiluminescence (ECL) self-interference spectroscopy technique (designated as ECLIS) with spatial resolution in the normal direction of electrode surface. Self-interference principally originates from superposition ECL emitted directly by luminophores and that reflected surfaces, resulting a spectrum consisting orderly distributed peaks. On basis this theoretical analysis matrix propagation model, distance between surface can be probed vertical on nanometer scale....