A5086825441- Adipose Tissue and Metabolism
- Muscle metabolism and nutrition
- Muscle Physiology and Disorders
- Mitochondrial Function and Pathology
- Metabolism, Diabetes, and Cancer
- Genetics, Aging, and Longevity in Model Organisms
- Genetics and Physical Performance
- Heat shock proteins research
- Tendon Structure and Treatment
- Healthcare Systems and Public Health
- ATP Synthase and ATPases Research
- Moyamoya disease diagnosis and treatment
- Hemodynamic Monitoring and Therapy
- FOXO transcription factor regulation
- Immune cells in cancer
- Radiation Dose and Imaging
- Metalloenzymes and iron-sulfur proteins
- Diet and metabolism studies
- Cardiac, Anesthesia and Surgical Outcomes
- Peroxisome Proliferator-Activated Receptors
- Cerebrovascular and genetic disorders
- Neurobiology and Insect Physiology Research
- Healthcare and Venom Research
- Nutrition and Health in Aging
- Blood Pressure and Hypertension Studies
Institute of Biomedical Problems
2015-2024
Skolkovo Institute of Science and Technology
2020
Research Center of Neurology
2018
Kirov State Medical Academy
2015
Ministry of Health of the Russian Federation
2015
Lomonosov Moscow State University
2014
Russian Academy of Sciences
2013
Evaluation of the influence primary and secondary aging on manifestation molecular cellular hallmarks is a challenging currently unresolved issue. Our study represents first demonstration distinct role chronic inflammation/physical inactivity - most important drivers aging, in regulation transcriptomic proteomic profiles human skeletal muscle. To achieve this purpose, young healthy people (n = 15), 8) older 37) patients with knee/hip osteoarthritis, model to effect long-term inflammation...
We investigated acute exercise-induced gene expression in skeletal muscle adapted to aerobic training. Vastus lateralis samples were taken ten endurance-trained males prior to, and just after, 4 h, 8 h after cycling sessions with different intensities, 70% 50% V˙O2max . High-throughput RNA sequencing was applied from two subjects evaluate differentially expressed genes intensive exercise (70% ), then the changes for selected validated by quantitative PCR (qPCR). To define genes, we compared...
Most studies examining the molecular mechanisms underlying adaptation of human skeletal muscles to aerobic exercise focused on response acute exercise. Here, we examined effect a 2-month training program baseline parameters in muscle. Ten untrained males performed one-legged knee extension for 1 h with same relative intensity before and after program. Biopsy samples were taken from vastus lateralis muscle at rest 2 month (baseline samples). Additionally, biopsy exercised leg 4 continuous...
ABSTRACT Introduction : We investigated the effect of resistance exercise intensity and exercise‐induced metabolic stress on activation anabolic signaling expression myogenic genes in skeletal muscle. Methods Ten strength‐trained athletes performed high‐intensity [HI, 74% 1‐repetition maximum (RM)], middle‐intensity (MI, 54% 1RM), or (54% 1RM) no‐relaxation (MIR). Kinase phosphorylation level gene muscle samples were evaluated before, 45 min, 5 h, 20 h after exercise. Results The lactate...
The goal of this study was to identify unknown transcription start sites the PPARGC1A ( PGC-1 α ) gene in human skeletal muscle and investigate promoter-specific regulation expression muscle. Ten amateur endurance-trained athletes performed high- low-intensity exercise sessions (70 min, 70% or 50% o 2max ). High-throughput RNA sequencing exon–exon junction mapping were applied analyse samples obtained at rest after exercise. activation regulators PGC-1α (AMPK, p38 MAPK, CaMKII, PKA CREB1)...
Promoter-specific expression of the PPARGC1A gene in untrained and trained human skeletal muscle was investigated. Ten males performed a one-legged knee extension exercise (for 60 min) with same relative intensity both before after 8 weeks cycling training. Samples from m. vastus lateralis each leg were taken exercise. Postexercise via canonical promoter increased by ~100% (P < 0.05) exercised nonexercised muscles, but did not change either training program. In muscle, alternative two orders...
In skeletal muscle of metabolically healthy subjects, a typical single meal normalized to body mass induces activation various transcription factors, expression numerous receptor tyrosine kinases associated with the insulin signaling cascade, and regulators. obese individuals without type 2 diabetes, this network is poorly regulated at transcriptional level, indicating dysregulation early gene response mixed meal.
Гипертрофический эффект силовых физических упражнений связывают с влиянием как механических, так и метаболических стимулов. При использовании в условиях ограниченного кровоснабжения работающих мышц, то есть условиях, когда метаболические сдвиги усиливаются, тренировочный может достигаться при гораздо меньших внешних отягощений (20% максимальной произвольной силы (МПС)). Были сопоставлены эффекты 8-недельной обычной высокоинтенсивной (8085% МПС) силовой тренировки низкой интенсивностью (50%...
The objective of the study is to evaluate diagnostic accuracy an original artificial intelligence (AI) algorithm for detecting MS in radiology department primary (outpatient) hospital. Materials and methods . Depersonalized results brain magnetic resonance imaging (MRI) studies performed period from August 22, 2019 September 26, 93 patients (42 men (mean age 47,5±15,9 years) 51 women 52,3±16,8 years)) were analyzed. All signed a voluntary informed consent form. Brain MRIwere carried out on...
Abstract Evaluation of the influence primary and secondary aging on manifestation molecular cellular hallmarks is a challenging currently unresolved issue. Our study represents first demonstration distinct role chronic inflammation/physical inactivity – most important drivers aging, in regulation transcriptomic proteomic profiles human skeletal muscle. To achieve this purpose, young healthy people (n=15), (n=8) older (n=37) patients with knee/hip osteoarthritis, model to effect long-term...
The functions of the Hsp70 genes were studied using a line D. melanogaster with knockout six these out thirteen. Namely, effect on negative geotaxis climbing (locomotor) speed and ability to adapt training (0.5-1.5 h/day, 7 days/week, 19 days) examined. Seven- 23-day-old - flies demonstrated comparable reduction (2-fold) in locomotor widespread changes leg skeletal muscle transcriptome (RNA-seq), compared w 1118 flies. To identify related decreased overlapped differentially expressed at both...
This study investigated the effect of knockout six Hsp70 genes (orthologues mammalian Hspa1a, Hspa1b, Hspa2, and Hspa8) on age-related changes in gene expression legs Drosophila melanogaster, which contain predominantly skeletal muscle bundles. For this, leg transcriptomic profile was examined males w^(1118) control strain Hsp70^(-) 7th, 23rd 47th days life. In flies, an decrease locomotion (climbing) speed (a marker functional state endurance) accompanied by a pronounced change muscles, is...
BACKGROUND. Skeletal muscles play a key role in the organism’s carbohydrate metabolism. Dysregulation of insulin-dependent glucose uptake skeletal muscle disrupts metabolism organism and can lead to development obesity type 2 diabetes. AIM. To identify expression markers (genes) human associated with disorders basal state after mixed meal normalized for body mass. MATERIALS AND METHODS . The study involved three groups 8 people: healthy volunteers, obese patients without Venous blood samples...
A decrease in muscle mass and its functionality (strength, performance insulin sensitivity) is one of the integral signs aging. One triggers aging an increase production mitochondrial reactive oxygen species. In our study, for first time, age-dependent changes species associated with a proportion mitochondria-associated hexokinase-2 human skeletal were studied. For this purpose, biopsy from m. vastus lateralis 10 young healthy volunteers 70 patients (26-85 years old) long-term primary...
Physical inactivity and aging cause significant impairments in the functionality mechanical properties of skeletal muscles, as well remodeling extracellular matrix (ECM). We aimed to study effect long-term age on biogenesis ECM muscle. For quantitative mass spectrometry-based proteomic analysis RNA sequencing, biopsy samples were taken from m. vastus lateralis 15 young healthy volunteers, 8 37 elderly patients with primary osteoarthritis knee/hip joint – which is a model for studying effects...
Purpose. To evaluate specific changes in MRI of the human brain, associated with cerebral autosomal dominant arteriopathy subcortical infarcts and leukoencephalopathy (CADASIL). Materials methods. We enrolled 24 patients genetically confirmed CADASIL (19–81 y.o.). The following sequences were performed for every subject: T1 MPR, T2, T2-FLAIR, DTI SWI. Brain tissue lesions assessed using STandards ReportIng Vascular on nEuroimaging (STRIVE). Results. In group observed (in % patients): recent...
Abstract The functions of the Hsp70 genes were studied using a line D. melanogaster with knockout six these out thirteen. Namely, effect on negative geotaxis climbing (locomotor) speed and ability to adapt training (0.5-1.5 h/day, 7 days/week, 19 days) examined. Seven- 23-day-old − flies demonstrated comparable reduction (2-fold) in locomotor widespread changes leg skeletal muscle transcriptome (RNA-seq), compared w 1118 flies. To identify related decreased overlapped differentially...