A5011049483- Microbial bioremediation and biosurfactants
- Microbial Community Ecology and Physiology
- Toxic Organic Pollutants Impact
- Wastewater Treatment and Nitrogen Removal
- Per- and polyfluoroalkyl substances research
- Microbial metabolism and enzyme function
- Microbial Fuel Cells and Bioremediation
- Environmental remediation with nanomaterials
- Water Treatment and Disinfection
- Atmospheric chemistry and aerosols
- Chemical Reactions and Isotopes
- Methane Hydrates and Related Phenomena
- Porphyrin Metabolism and Disorders
- Chemistry and Chemical Engineering
- Microbial Metabolic Engineering and Bioproduction
- Chemical Analysis and Environmental Impact
- Bioeconomy and Sustainability Development
- Effects and risks of endocrine disrupting chemicals
- Chromium effects and bioremediation
- Gut microbiota and health
- Atmospheric and Environmental Gas Dynamics
- Genomics and Phylogenetic Studies
- Tracheal and airway disorders
- Arsenic contamination and mitigation
- Geochemistry and Elemental Analysis
Lawrence Berkeley National Laboratory
2016-2025
University of California, Berkeley
2016-2025
State Key Laboratory of Pollution Control and Resource Reuse
2010-2018
Tongji University
2010-2018
Berkeley College
2016
National University of Singapore
2015
Chinese Research Academy of Environmental Sciences
2010
NASA Earth Science
2009
Technical University of Munich
2009
Brown and Caldwell (United States)
2004
Abstract The Deepwater Horizon (DWH) oil spill in the spring of 2010 resulted an input ∼4.1 million barrels to Gulf Mexico; >22% this is unaccounted for, with unknown environmental consequences. Here we investigated impact deposition on microbial communities surface sediments collected at 64 sites by targeted sequencing 16S rRNA genes, shotgun metagenomic 14 these samples and mineralization experiments using 14C-labeled model substrates. gene data indicated that most heavily...
Polybrominated diphenyl ethers (PBDEs) are a class of widely used flame retardants that have recently been detected in environmental samples, diverse biota, human blood serum, and breast milk at exponentially increasing concentrations. Currently, little is known about the fate these compounds, particular, microbial potential to degrade them. In this study, debromination deca-BDE an octa-BDE mixture demonstrated with anaerobic bacteria including Sulfurospirillum multivorans Dehalococcoides...
It is now well accepted that the gut microbiota contributes to our health. However, what determines composition still unclear. Whereas it might be expected intestinal niche would dominant in shaping microbiota, studies vertebrates have repeatedly demonstrated effects of external factors such as host diet and environmental microbial diversity. Hypothesizing genetic variation may interfere with discerning contributions factors, we turned Caenorhabditis elegans a new model, offering ability...
The aerobic biotransformation pathways of 4:2, 6:2, and 8:2 fluorotelomer thioether amido sulfonate (FtTAoS) were characterized by determining the fate compounds in soil medium microcosms amended with an aqueous film-forming foam (AFFF) solution. FtTAoS occurred live over approximately 40 days produced (FtS), 6:2 unsaturated carboxylic acid (FtUCA), 5:3 (FtCA), C4 to C8 perfluorinated acids (PFCAs). Two products corresponding singly doubly oxygenated forms also identified through high...
The trichloroethylene (TCE) transformation rate and capacity of a mixed methanotrophic culture at room temperature were measured to determine the effects time without methane (resting), use an alternative energy source (formate), aeration, toxicity TCE its products. initial specific resting cells was 0.6 mg per day, they had finite 0.036 cells. Formate addition resulted in increased rates (2.1 mg/mg day) elevated (0.073 cells). Significant declines conversion following exposure observed for...
The rate and capacity for chloroform (CF) trichloroethylene (TCE) transformation by a mixed methanotrophic culture of resting cells (no exogenous energy source) formate-fed were measured. As reported previously TCE, formate addition resulted in an increased CF (0.35 day-1 1.5 cells) (0.0065 mg per 0.015 cells), suggesting that depletion stores affects behavior. observed finite capacity, even with source, suggests toxicity was also factor. significantly lower than greater from transformation....
The debromination pathways of seven polybrominated diphenyl ethers (PBDEs) by three different cultures anaerobic dehalogenating bacteria were investigated using comprehensive two-dimensional gas chromatography (GC × GC). congeners analyzed the five major components industrially used octa-BDE mixture (octa-BDEs 196, 203, and 197, hepta-BDE 183, hexa-BDE 153) as well two most commonly detected PBDEs in environment, penta-BDE 99 tetra-BDE 47. Among evaluated this study a...
Current knowledge of the microbial communities within biological wastewater treatment reactors is incomplete due to limitations traditional culture-based techniques and despite emergence recently applied molecular techniques. Here we demonstrate application high-density microarrays targeting universal 16S rRNA genes evaluate community composition in five China United States. Results suggest a surprisingly consistent structure among all reactors. All investigated contained core bacterial...
An anaerobic microbial consortium (referred to as ANAS) that reductively dechlorinates trichloroethene (TCE) completely ethene with the transient production of cisdichloroethene (cDCE) and vinyl chloride was enriched from contaminated soil obtained Alameda Naval Air Station. ANAS uses lactate its electron donor has been functionally stable for over 2 years. Following a brief exposure oxygen, subculture (designated VCC) derived could dechlorinate TCE only donor. Three molecular methods were...
Bacteria belonging to the genus Dehalococcoides play a key role in complete detoxification of chloroethenes as these organisms are only microbes known be capable dechlorination beyond dichloroethenes vinyl chloride (VC) and ethene. However, strains usually grow slowly with doubling time 1 2 days have complex nutritional requirements. Here we describe growth ethenogenes 195 defined mineral salts medium, improved strain when medium was amended high concentrations vitamin B(12), strategy for...
1,4-Dioxane is a probable human carcinogen, and an important emerging water contaminant. In this study, the biodegradation of dioxane by 20 bacterial isolates was evaluated, 13 were found to be capable transforming dioxane. Dioxane served as growth substrate for Pseudonocardia dioxanivorans CB1190 benzenivorans B5, with yields 0.09 g protein dioxane-1 0.03 dioxane-1, respectively. Cometabolic transformation observed monooxygenase-expressing strains that induced methane, propane,...
Three fungal strains were found to be capable of bioleaching rare earth elements from monazite, a phosphate mineral, utilizing the monazite as source and releasing cations into solution. These organisms include one known solubilizing fungus, Aspergillus niger ATCC 1015, well two newly isolated fungi: an terreus strain ML3-1 Paecilomyces spp. WE3-F. Although also contains radioactive element Thorium, by these fungi preferentially solubilized over leaving Thorium in solid residual. Adjustments...
ABSTRACT Quantitative PCR (qPCR) was coupled with reverse transcription (RT) to analyze both gene copy numbers and transcripts of the 16S rRNA three reductive dehalogenase (RDase) genes ( tceA , vcrA bvcA ) as biomarkers “ Dehalococcoides ” spp. in groundwater a trichloroethene-dense nonaqueous-phase liquid site at Fort Lewis, WA, that sequentially subjected biostimulation bioaugmentation. cells carrying were indigenous site. The sum identified RDase closely correlated throughout...
Understanding microbial interactions in engineering bioprocesses is important to enhance and optimize performance outcomes requires dissection of the multi-layer complexities communities. However, unraveling as well substrates involved complex communities a challenging task. Here, we demonstrate an integrated approach metagenomics, metatranscriptomics, targeted metabolite analysis identify interspecies from potential cross-feeding model community-bisphenol A (BPA)-biodegrading community,...
Polybrominated diphenyl ethers (PBDEs) are flame retardants that have been used in consumer products and furniture for three decades. Currently, very little is known about their fate the environment specifically susceptibility to aerobic biotransformation. Here, we investigated ability of polychlorinated biphenyl (PCB) degrading bacteria Rhodococcus jostii RHA1 Burkholderia xenovorans LB400 transform mono- through hexa-BDEs at ppb levels. We also tested PBDE transforming abilities related...
Corrinoids are cobalt-containing molecules that function as enzyme cofactors in a wide variety of organisms but produced solely by subset prokaryotes. Specific corrinoids identified the structure their axial ligands. The lower ligand corrinoid can be benzimidazole, purine, or phenolic compound. Though it is known many obtain from environment, serve for any one organism largely unstudied. Here, we examine range corrinoid-dependent metabolism Dehalococcoides mccartyi strain 195. bacteria play...