A5039670201- Wastewater Treatment and Nitrogen Removal
- Microbial Community Ecology and Physiology
- Microbial Fuel Cells and Bioremediation
- Anaerobic Digestion and Biogas Production
- Genomics and Phylogenetic Studies
- Gut microbiota and health
- Methane Hydrates and Related Phenomena
- Chromium effects and bioremediation
- Water Treatment and Disinfection
- Electrochemical sensors and biosensors
- Constructed Wetlands for Wastewater Treatment
- Extraction and Separation Processes
- Membrane-based Ion Separation Techniques
- Tracheal and airway disorders
- Polar Research and Ecology
- Health, Environment, Cognitive Aging
- Cell Image Analysis Techniques
- Phosphorus and nutrient management
- CO2 Reduction Techniques and Catalysts
- Protist diversity and phylogeny
- Microbial Metabolic Engineering and Bioproduction
Universidad ORT Uruguay
2015-2023
Instituto de Investigaciones Biológicas Clemente Estable
2015-2019
Max Planck Institute for Terrestrial Microbiology
2010-2015
Universidad de la República de Uruguay
2006-2009
Universidad La República
2009
Universidad de Montevideo
2006
Centro Uruguayo de Imagenología Molecular
2002
Abstract Background The phylum Chloroflexi is highly abundant in a wide variety of wastewater treatment bioreactors. It has been suggested that they play relevant roles these ecosystems, particularly degrading carbon compounds and on structuring flocs or granules. Nevertheless, their function not yet well understood as most species have isolated axenic cultures. Here we used metagenomic approach to investigate diversity metabolic potential three environmentally different bioreactors:...
ABSTRACT By placing the anode of a sediment microbial fuel cell (SMFC) in rhizosphere rice plant, root-excreted rhizodeposits can be microbially oxidized with concomitant current generation. Here, various molecular techniques were used to characterize composition bacterial and archaeal communities on such anodes, as influenced by electrical circuitry, matrix, presence plants. Closed-circuit anodes potting soil enriched Desulfobulbus -like species, members family Geobacteraceae , yet...
Plant-based sediment microbial fuel cells (PMFCs) couple the oxidation of root exudates in living rice plants to current production. We analysed composition community on anodes from PMFC with natural field soil as substratum for by analysing 16S rRNA an indicator activity and diversity. Terminal restriction fragment length polymorphism (TRFLP) analysis indicated that active bacterial PMFCs differed strongly compared controls. Moreover, clones related Deltaproteobacteria Chloroflexi were...
The phylum Chloroflexi is frequently found in high abundance methanogenic reactors, but their role still unclear as most of them remain uncultured and understudied. Hence, a detailed analysis was performed samples from five up-flow anaerobic sludge blanket (UASB) full-scale reactors fed different industrial wastewaters.Quantitative PCR show that the abundant all UASB with higher operated for long period time, which presented granular biomass. Both terminal restriction fragment length...
Although microbial communities of anaerobic bioreactors have been extensively studied using DNA-based tools, there are still several knowledge gaps regarding the microbiology process, in particular integration all generated data is limited. One understudied core phylum within Chloroflexi, despite being one most abundant groups reactors. In order to address abundance, diversity and phylogeny this group full-scale methanogenic reactors globally distributed, a compilation 16S ribosomal RNA gene...
Ca. Accumulibacter was the predominant microorganism (relative FISH bio-abundance of 67 ± 5%) in a lab-scale sequential batch reactor that accomplished enhanced biological phosphorus removal (EBPR) while using glucose and acetate as carbon sources (1:1 COD-based ratio). Both organic compounds were completely anaerobically consumed. The reactor's performance terms P/C ratio, phosphorous release uptake, overall kinetic stoichiometric parameters on high end reported spectrum for EBPR systems...
Abstract The phylum Chloroflexota has been found to exhibit high abundance in the microbial communities from wastewater treatment plants (WWTPs) both aerobic and anaerobic systems. However, its metabolic role not fully explored due lack of cultured isolates. To address this gap, we use publicly available metagenome datasets activated sludge (AS) methanogenic (MET) full-scale reactors assembled genomes. Using strategy, 264 dereplicated, medium- high-quality metagenome-assembled genomes (MAGs)...
Rare members of environmental microbial communities are often overlooked and unexplored, primarily due to the lack techniques capable acquiring their genomes. Chloroflexi belong one most understudied phyla, even though many its ubiquitous in environment some play important roles biochemical cycles or biotechnological applications. We here used a targeted cell sorting approach, which enables selection specific taxa by fluorescent labelling is compatible with subsequent single genomics, enrich...
This study aims at elucidating the metabolic pathways involved in production of volatile fatty acids from CO2 and electricity. Two bioelectrochemical systems (BES) were fed with pure (cells A B). The cathode potential was first poised -574 mV vs. standard hydrogen electrode (SHE) then -756 SHE order to ensure required reducing power. Despite applying similar operation conditions both BES, they responded differently. mixture organic compounds (1.87 mM acetic acid, 2.30 formic 0.43 propionic...
Nitrogen cycle has been poorly investigated in Antarctic ecosystems. In particular, how extreme conditions of low temperature, dryness, and high radiation select the microorganisms involved is not yet understood. Denitrification an important step nitrogen which nitrate reduced stepwise to gases NO, N2O, N2. carried out by a wide group spread phylogenetic tree. The aim this work was isolate characterize denitrifying bacteria present different cold environments from Antarctica. Bacterial...
The microbial community in two acetate-fed denitrifying reactors, inoculated with methanogenic sludge, was monitored by 16S rDNA-based methods (SSCP and FISH). Both reactors converged to similar, stable communities. predominant organisms belonged the genera Thauera, Paracoccus Denitrobacter, detected both molecular culture-based methods.
The treatment of dairy wastewater in methanogenic reactors cause several problems due to their high lipid content. One strategy overcome these is the use commercial formulations. Here we studied effect adding a formulation, designed improve fat degradation, on both microbial community composition and reactor performance. Samples from two full-scale Up-flow Anaerobic Sludge Blanket (UASB) parallel arrangement were analysed. product was added one while other used as control. amendment...
Aerobic and methanogenic consortia were evaluated as inocula for laboratory scale denitrifying reactors, fed with a synthetic wastewater acetate the main electron donor. The microflora of reactors was by specific activity, enumeration isolation denitrifiers, which screened amplified ribosomal DNA restriction analysis. Reactor performance monitored COD nitrate removal efficiencies granule size. aerobic sludge failed to form granules, probably due development filamentous, nitrate-reducing...
Slaughterhouse wastewater is a complex effluent with an important content of organic nitrogen. After anaerobic treatment where most the matter removed, nitrogen, remains as ammonium and post-treatment necessary. Sequencing batch reactor (SBR) technology has been developed to completely remove nitrogen in one single combining aerobic anoxic stages. Under oxygen limited conditions only nitrite produced concomitant energy saving. The stability diversity microbial community from nitrifying...
Wastewater from dairy industries, characterized by its high COD content and relative COD/TKN ratio, requires post-treatment after anaerobic treatment to complete the removal of organic matter nutrients. Due simplicity, robustness low maintenance costs, sequencing batch reactors (SBR) result in an attractive system, especially case small industries order comply with emission standards. The goal this work was determine operational parameters, optimize performance, study stability microbial...