A5026841981- Immune Cell Function and Interaction
- Food Allergy and Anaphylaxis Research
- Drug-Induced Adverse Reactions
- Asthma and respiratory diseases
- Contact Dermatitis and Allergies
- Urticaria and Related Conditions
- Immunotherapy and Immune Responses
- Autoimmune Bullous Skin Diseases
- melanin and skin pigmentation
- Pharmaceutical studies and practices
- Dermatology and Skin Diseases
- Cytokine Signaling Pathways and Interactions
- T-cell and B-cell Immunology
- Occupational exposure and asthma
- IL-33, ST2, and ILC Pathways
- Allergic Rhinitis and Sensitization
- Sulfur Compounds in Biology
- Fibroblast Growth Factor Research
- Pharmacological Effects and Toxicity Studies
- NF-κB Signaling Pathways
- Regulation of Appetite and Obesity
- Skin Protection and Aging
- Multiple Sclerosis Research Studies
- Biochemical Analysis and Sensing Techniques
- T-cell and Retrovirus Studies
Southampton General Hospital
2006-2017
University of Southampton
2002-2017
University Hospital Southampton NHS Foundation Trust
2012
St James's University Hospital
1997-1999
The number of type 2 helper CD4+ T cells is increased in the airways persons with asthma. Whether majority these are class II major-histocompatibility-complex-restricted or among recently identified CD1d-restricted invariant natural killer a matter controversy. We studied frequency subjects mild moderately severe asthma to investigate possibility an association between airway and disease severity. also whether feature chronic obstructive pulmonary (COPD).We enumerated by flow cytometry use...
Previous reports have suggested that drug-specific lymphocyte proliferation assays (LPA) can be used retrospectively to confirm the culprit drug following delayed-type hypersensitivity reactions (DHR). However, only limited evidence supports their use in aiding acute clinical management. The aim of this study was compare LPA against combination cytokine for potential setting.A total 43 patients with DHR (19 during reaction, 20 after recovery, four and recovery) 14 control subjects without...
Abstract Studies in mice indicate that α-melanocyte-stimulating hormone (αMSH) is immunosuppressive, but it not known whether αMSH suppresses human immune responses to exogenous Ags. Human PBMCs, including monocytes, express the melanocortin 1 receptor (MC1R), and thought ability of alter monocyte-costimulatory molecule expression IL-10 release mediated by this receptor. However, MC1R gene polymorphic, certain variants compromise signaling via cAMP, resulting red hair fair skin. Here, we...
Abstract Contact allergy to environmental xenobiotics is a common and important problem, but it unclear why some chemicals are potent sensitizers others weak/nonsensitizers. We explored this by investigating similar chemicals, 2,4-dinitrochlorobenzene (DNCB) 2,4-dinitrothiocyanobenzene (DNTB), differ in their ability induce contact hypersensitivity (CHS). DNCB induced CHS humans, whereas at doses DNTB did not. However, following sensitization, elicited vivo stimulated DNCB-responsive T cells...
Background Contact sensitization by ingredients in personal products is an important clinical problem. It not clear how induced the generally low concentrations at which they occur but it might be result of repeated exposure. Objectives To compare strength contact a single exposure to 2,4‐dinitrochlorobenzene (DNCB) (60 μg cm−2) or three exposures subsensitizing dose (10 cm−2). Methods Two groups (n =10) healthy adult volunteers were randomized receive either patch DNCB 60 cm−2 once‐weekly...
Abstract Lung fibroblasts are key structural cells that reside in the submucosa where they contact with large numbers of CD4+ Th cells. During severe viral infection and chronic inflammation, is susceptible to bacterial invasion by lung microbiota such as nontypeable Haemophilus influenzae (NTHi). Given their proximity tissue, we hypothesized human play an important role modulating cell responses NTHi. We demonstrate express critical T Ag-presentation molecule HLA-DR within lung, this...
SUMMARY In this study we examined the cytokine production by T cells and TCRVβ subsets in peripheral blood (PB) synovial fluid (SF) from six RA patients PB 10 normal subjects, using three-colour flow cytometry. two subjects assessed cell clonality RT PCR TCRBV family-specific primers analysed CDR3 (complementarity determining region 3) length GeneScan analysis. A high percentage of IFN-γ- IL-2- producing was observed among both controls SF patients. contrast, IL-4 IL-5 small There no...
Abstract Background The signal transducer and activator of transcription‐4 ( STAT 4/Stat4) is a transcription factor known to convey signals from interleukin‐12, interleukin‐23, interferon‐alpha/beta the nucleus, resulting in activation dendritic cells, T‐helper cell differentiation production interferon‐gamma. Objective To demonstrate novel role for 4 mitosis. Results Phosphoserine pS er 4) increased cells undergoing mitosis distributed throughout cytoplasm during this stage cycle, whilst...
T cells are thought to play an important regulatory role in atopic asthma. We hypothesized that human blood and BAL cell subsets bearing various TCR-Vbeta genes might show selective differences their cytokine profile. Peripheral (PB) bronchoalveolar lavage (BAL) from seven asthmatic six non-atopic non-asthmatic subjects were stimulated with PMA ionomycin the presence of monensin analysed for expression production cytokines at single level. The percentage IFN-gamma- IL-2-producing was...
<h3>Introduction</h3> Chronic obstructive pulmonary disease (COPD) is a chronic inflammatory condition characterised by increased numbers of neutrophils, macrophages and CD8<sup>+</sup> T cells. However, recent evidence suggests that both CD4<sup>+</sup> cells can influence the pathogenesis disease. We aim to characterise cell subsets from airway parenchyma. <h3>Method</h3> Macroscopically normal lung parenchyma bronchial tissue was obtained 10 patients (average age=65.3±4.5 years,...